CLC number: Q966
On-line Access: 2010-03-29
Received: 2009-10-30
Revision Accepted: 2010-02-01
Crosschecked: 2010-03-05
Cited: 9
Clicked: 6252
Hao Yin, Xin-qin Shi, Bo Sun, Jing-jing Ye, Zu-an Duan, Xiao-ling Zhou, Wei-zheng Cui, Xiao-feng Wu. Accumulation of 1-deoxynojirimycin in silkworm, Bombyx mori L.[J]. Journal of Zhejiang University Science B, 2010, 11(4): 286-291.
@article{title="Accumulation of 1-deoxynojirimycin in silkworm, Bombyx mori L.",
author="Hao Yin, Xin-qin Shi, Bo Sun, Jing-jing Ye, Zu-an Duan, Xiao-ling Zhou, Wei-zheng Cui, Xiao-feng Wu",
journal="Journal of Zhejiang University Science B",
volume="11",
number="4",
pages="286-291",
year="2010",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0900344"
}
%0 Journal Article
%T Accumulation of 1-deoxynojirimycin in silkworm, Bombyx mori L.
%A Hao Yin
%A Xin-qin Shi
%A Bo Sun
%A Jing-jing Ye
%A Zu-an Duan
%A Xiao-ling Zhou
%A Wei-zheng Cui
%A Xiao-feng Wu
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 4
%P 286-291
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0900344
TY - JOUR
T1 - Accumulation of 1-deoxynojirimycin in silkworm, Bombyx mori L.
A1 - Hao Yin
A1 - Xin-qin Shi
A1 - Bo Sun
A1 - Jing-jing Ye
A1 - Zu-an Duan
A1 - Xiao-ling Zhou
A1 - Wei-zheng Cui
A1 - Xiao-feng Wu
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 4
SP - 286
EP - 291
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0900344
Abstract: 1-deoxynojirimycin (1-DNJ) contents in the silkworm, Bombyx mori, at different developmental stages and tissues were investigated by using reverse-phase high-performance liquid chromatography. The 1-DNJ contents of silkworm larvae change significantly with their developmental stages. The male larvae showed higher accumulation efficiency of 1-DNJ than the females and also a significant variation was observed among the silkworm strains. The present results show that tissue distribution of 1-DNJ was significantly higher in blood, digestive juice, and alimentary canal, but no 1-DNJ was observed in the silkgland. Moreover, 1-DNJ was not found in silkworms fed with artificial diet that does not contain mulberry leaf powder. This proves that silkworms obtain 1-DNJ from mulberry leaves; they could not synthesize 1-DNJ by themselves. The accumulation and excretion of 1-DNJ change periodically during the larval stage. There was no 1-DNJ in the newly-hatched larvae and 1-DNJ was mainly accumulated during the early and middle stages of every instar, while excreted at later stages of larval development. Further, it is possible to extract 1-DNJ from the larval feces and it is optimal to develop the 1-DNJ related products for diabetic auxiliary therapy.
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