CLC number: R446.11+9
On-line Access:
Received: 2003-06-10
Revision Accepted: 2003-08-07
Crosschecked: 0000-00-00
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YANG Yun-mei, CAO Hong-cui, XU Zhe-rong, CHEN Xiao-ming. Creation of reversed phase high-performance liquid chromatographic technique to assay platelet-activating factor[J]. Journal of Zhejiang University Science A, 2004, 5(6): 738-742.
@article{title="Creation of reversed phase high-performance liquid chromatographic technique to assay platelet-activating factor",
author="YANG Yun-mei, CAO Hong-cui, XU Zhe-rong, CHEN Xiao-ming",
journal="Journal of Zhejiang University Science A",
volume="5",
number="6",
pages="738-742",
year="2004",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2004.0738"
}
%0 Journal Article
%T Creation of reversed phase high-performance liquid chromatographic technique to assay platelet-activating factor
%A YANG Yun-mei
%A CAO Hong-cui
%A XU Zhe-rong
%A CHEN Xiao-ming
%J Journal of Zhejiang University SCIENCE A
%V 5
%N 6
%P 738-742
%@ 1869-1951
%D 2004
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2004.0738
TY - JOUR
T1 - Creation of reversed phase high-performance liquid chromatographic technique to assay platelet-activating factor
A1 - YANG Yun-mei
A1 - CAO Hong-cui
A1 - XU Zhe-rong
A1 - CHEN Xiao-ming
J0 - Journal of Zhejiang University Science A
VL - 5
IS - 6
SP - 738
EP - 742
%@ 1869-1951
Y1 - 2004
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2004.0738
Abstract: Objective: To establish a new assay for platelet-activating factor (PAF), to compare it with bio-assay; and to discuss its significance in some elderly people diseases such as cerebral infarction and coronary heart disease. Methods: To measure PAF levels in 100 controls, 23 elderly patients with cerebral infarction and 65 cases with coronary heart disease by reversed phase high-performance liquid chromatographic technique (rHPLC). Results: rHPLC is more convenient, sensitive, specific, and less confusing, compared with bio-assay. The level of plasma PAF in patients with cerebral infarction was higher than that in the controls (P<0.01), and in patients with coronary heart disease. Conclusion: Detection of PAF with rHPLC is more reliable and more accurate. The new assay has important significance in PAF research.
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