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CLC number: R722.13+1

On-line Access: 2024-08-27

Received: 2023-10-17

Revision Accepted: 2024-05-08

Crosschecked: 0000-00-00

Cited: 9

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Citations:  Bibtex RefMan EndNote GB/T7714

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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.1 P.53-56

http://doi.org/10.1631/jzus.2005.B0053


The binding of MBL to common bacteria in infectious diseases of children


Author(s):  SHANG Shi-qiang, CHEN Guo-xian, SHEN Jie, YU Xiao-hong, WANG Ke-yi

Affiliation(s):  Affiliated Children’ more

Corresponding email(s):   Shangsq33@mail.hz.zj.cn

Key Words:  Mannan-binding lectin (MBL), Children, Innate immunity, Bacteria



Abstract: 
Objective: To purify mannan-binding lectin (MBL) from human serum and detect its binding ability to several kinds of bacteria common in infectious diseases of children. Methods: MBL was purified from human serum by affinity chromatography on mannan-Sepharose 4B column. Its binding ability to eight species, 97 strains of bacteria was detected by enzyme-linked lectin assay (ELLA). Results: MBL has different binding ability to bacteria and shows strong binding ability to Klebsiella ornithinolytica and Escherichia coli, but shows relatively lower binding ability to Staphylococcus haemolyticus, Enterobacter cloacae and Staphylococcus epidermidis. To different isolates of Klebsiella pneumoniae, Haemophilus influenzae and Staphylococcus aureus, MBL shows quite different binding ability. Conclusions: MBL has different binding ability to different bacteria, and has relatively stronger binding ability to Gram-negative bacteria. Its binding ability to different isolates of certain kinds of bacteria is quite different.

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