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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.12 P.1141-1147

http://doi.org/10.1631/jzus.2005.B1141


Study on the expression and mutation of human telomeric repeat binding factor (hTRF1) in 10 malignant hematopoietic cell lines


Author(s):  SUN Jie, HUANG He, ZHU Yuan-yuan, LAN Jian-ping, LI Jing-yuan, LAI Xiao-yu, YU Jian

Affiliation(s):  Department of Hematology, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China

Corresponding email(s):   hehuangyu@126.com

Key Words:  Human telomeric repeat binding factor (hTRF1), Expression, Mutation, Genome, Processed pseudogene


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SUN Jie, HUANG He, ZHU Yuan-yuan, LAN Jian-ping, LI Jing-yuan, LAI Xiao-yu, YU Jian. Study on the expression and mutation of human telomeric repeat binding factor (hTRF1) in 10 malignant hematopoietic cell lines[J]. Journal of Zhejiang University Science B, 2005, 6(12): 1141-1147.

@article{title="Study on the expression and mutation of human telomeric repeat binding factor (hTRF1) in 10 malignant hematopoietic cell lines",
author="SUN Jie, HUANG He, ZHU Yuan-yuan, LAN Jian-ping, LI Jing-yuan, LAI Xiao-yu, YU Jian",
journal="Journal of Zhejiang University Science B",
volume="6",
number="12",
pages="1141-1147",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B1141"
}

%0 Journal Article
%T Study on the expression and mutation of human telomeric repeat binding factor (hTRF1) in 10 malignant hematopoietic cell lines
%A SUN Jie
%A HUANG He
%A ZHU Yuan-yuan
%A LAN Jian-ping
%A LI Jing-yuan
%A LAI Xiao-yu
%A YU Jian
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 12
%P 1141-1147
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B1141

TY - JOUR
T1 - Study on the expression and mutation of human telomeric repeat binding factor (hTRF1) in 10 malignant hematopoietic cell lines
A1 - SUN Jie
A1 - HUANG He
A1 - ZHU Yuan-yuan
A1 - LAN Jian-ping
A1 - LI Jing-yuan
A1 - LAI Xiao-yu
A1 - YU Jian
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 12
SP - 1141
EP - 1147
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B1141


Abstract: 
Objective: Detecting the expression and mutation of human telomeric repeat binding factor (hTRF1) in 10 malignant hematopoietic cell line cells on the base of determining its genomic structure and its four pseudogenes to clarify if hTRF1 mutation is one of the factors of the activation of telomerase. Methods: hTRF1cDNA sequences were obtained from GenBank, its genome structure and pseudogenes were forecasted by BLAST and other biology information programs and then testified by sequencing. Real-time RT-PCR was used to detect the expression of hTRF1mRNA in 10 cell line cells, including myelogenous leukemia cell lines K562, HL-60, U-937, NB4, THP-1, HEL and Dami; lymphoblastic leukemia cell lines 6T-CEM, Jurkat and Raji. Telomerase activities of cells were detected by using telomeric repeat amplification (TRAP)-ELISA protocol. PCR and sequencing were used to detect mutation of each exon of hTRF1 in 10 cell line cells. Results: hTRF1 gene, mapped to 8q13, was divided into 10 exons and spans 38.6 kb. Four processed pseudogenes of hTRF1 located on chromosome 13, 18, 21 and X respectively, was named as ΨhTRF1-13, ΨhTRF1-18, ΨhTRF1-21 and ΨhTRF1-X respectively. All cell line cells showed positive telomerase activity. The expression of hTRF1 was significantly lower in malignant hematopoietic cell lines cells (0.0338, 0.0108~0.0749) than in normal mononuclear cells (0.0493, 0.0369~0.128) (P=0.004). But no significant mutation was found in all exons of hTRF1 in 10 cell line cells. Four variants were found in part of intron 1, 2 and 8 of hTRF1. Their infection on gene function is unknown and needs further studies. Conclusion: hTRF1 mutation is probably not one of the main factors for telomerase activation in malignant hematopoietic disease.

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