Full Text:   <3445>

CLC number: X53

On-line Access: 2024-08-27

Received: 2023-10-17

Revision Accepted: 2024-05-08

Crosschecked: 0000-00-00

Cited: 28

Clicked: 7507

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.5 P.311-318

http://doi.org/10.1631/jzus.2005.B0311


Accumulation and ultrastructural distribution of copper in Elsholtzia splendens


Author(s):  PENG Hong-yun, YANG Xiao-e, TIAN Sheng-ke

Affiliation(s):  Ministry of Education Key Lab of Environment, Remediation and Ecosystem Health, School of Natural Resources and Environmental Science, Zhejiang University, Hangzhou 310029, China

Corresponding email(s):   penghongyun@zju.edu.cn, xyang@zju.edu.cn

Key Words:  Cell wall, Chloroplast, Cu detoxification, Elsholtzia splendens, Ultrastructural distribution


PENG Hong-yun, YANG Xiao-e, TIAN Sheng-ke. Accumulation and ultrastructural distribution of copper in Elsholtzia splendens[J]. Journal of Zhejiang University Science B, 2005, 6(5): 311-318.

@article{title="Accumulation and ultrastructural distribution of copper in Elsholtzia splendens",
author="PENG Hong-yun, YANG Xiao-e, TIAN Sheng-ke",
journal="Journal of Zhejiang University Science B",
volume="6",
number="5",
pages="311-318",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B0311"
}

%0 Journal Article
%T Accumulation and ultrastructural distribution of copper in Elsholtzia splendens
%A PENG Hong-yun
%A YANG Xiao-e
%A TIAN Sheng-ke
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 5
%P 311-318
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0311

TY - JOUR
T1 - Accumulation and ultrastructural distribution of copper in Elsholtzia splendens
A1 - PENG Hong-yun
A1 - YANG Xiao-e
A1 - TIAN Sheng-ke
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 5
SP - 311
EP - 318
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0311


Abstract: 
Copper accumulation and intracellular distribution in Elsholtzia splendens, a native Chinese Cu-tolerant and accumulating plant species, was investigated by transmission electron microscope (TEM) and gradient centrifugation techniques. Copper concentrations in roots, stems and leaves of E. splendens increased with increasing Cu levels in solution. After exposure to 500 μmol/L Cu for 8 d, about 1000 mg/kg Cu were accumulated in the stem and 250 mg/kg Cu in the leaf of E. splendens. At 50 μmol/L Cu, no significant toxicity was observed in the chloroplast and mitochondrion within its leaf cells, but separation appeared at the cytoplasm and the cell wall within the root cells. At >250 μmol/L Cu, both root and leaf cell organelles in E. splendens were damaged heavily by excessive Cu in vivo. Copper subcellular localization in the plant leaf after 8 days’ exposure to 500 μmol/L Cu using gradient centrifugation techniques was found to be decreased in the order: chloroplast>cell wall>soluble fraction>other organelles. The plant root cell wall was found to be the site of highest Cu localization. Increase of Cu exposure time from 8 d to 16 d, increased slightly Cu concentration in cell wall fraction in roots and leaves, while that in the chloroplast fraction decreased in leaves of the plants grown in both 0.25 μmol/L and 500 μmol/L Cu. TEM confirmed that much more Cu localized in cell walls of E. splendens roots and leaves, but also more Cu localized in E. splendenschloroplast when the plant is exposed to Cu levels>250 μmol/L, as compared to those in the plant grown in 0.25 μmol/L Cu. Copper treatment at levels>250 μmol/L caused pronounced damage in the leaf chloroplast and root organelles. Copper localization in cell walls and chloroplasts could mainly account for the high detoxification of Cu in E. splendens.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1] Allan, D.L., Jarrell, W.M., 1989. Proton and copper adsorption to maize and soybean root cell wall. Plant Physiology, 89:823-832.

[2] Brooks, R.R., Shaw, S., Marfil, A.A., 1981. The chemical form and physiological function of nickel in some Iberian Alyssum species. Physiologial Plantarum, 51:167-170.

[3] Carroll, M., 1989. Organelles. The Guiford Press, London.

[4] Cathala, N., Salsac, L., 1975. Absorption of copper by roots of corn (Zea mays) and sunflower (Helianthus annuus). Plant and Soil, 42:65-83.

[5] De Vos, C.H.R., Schat, H., Vooijs, R., Ernst, W.H.O., 1989. Copper-induced damage to the permeability barrier in roots of Silene cucubalus. Journal of plant physiology, 135:165-169.

[6] De Vos, C.H.R., Schat, H., De Waal, M.A.M., Vooijs, R., Ernst, W.H.O., 1991. Increased resistance to copper-induced damage of the root cell plasmalemma in copper-tolerant Silene cucubalus. Physiologia Plantarum, 82:523-528.

[7] Ernst, W.H.O., Verkleij, J.A.C., Schat, H., 1992. Metal tolerance in plants. Acta Botanica Neerlandica, 41:229-248.

[8] Halliwell, B., Gutteridge, J.M., 1984. Oxygen toxicity, oxygen radicals, transition metals and disease. Biochemistry Journal, 219:1-14.

[9] Harrison, S.J., Lepp, N.W., Phipps, D.A., 1984. Uptake of copper by excised roots. II. Copper desorption from the free space. Z Pflanzenernähr Bodenkd, 94:27-34.

[10] Hayens, R.J., 1980. Ion exchange properties of roots and ionic interactions within the root POPLsm: Their role in ion accumulation by plants. Botany Review, 46:75-99.

[11] Henriques, F.S., 1989. Effects of copper deficiency on the photosynthetic apparatus of sugar beet (Beta vulgaris L.). Journal of plant physiology, 135:453-458.

[12] Iwasaki, K., Sakurai, K., Takahashi, E., 1990. Copper binding by the root cell walls of Italian ryegrass and red clover. Soil Science and Plant Nutrition, 36(3):431-439.

[13] Kennedy, C.D., Gonsalves, F.A.N., 1987. The action of divalent zinc, cadmium, mercury, copper and lead on the trans-root potential and H+ efflux of excised roots. Journal of Experimental Botany, 38:800-817.

[14] Krämer, U., Pickering, I.J., Prince, R.C., Raskin, I., Salt, D.E., 2000. Subcellular localization and speciation of nickel in hyperaccumulator and non-accumulator Thlaspi species. Plant Physiology, 122:1343-1353.

[15] Krotz, R.M., Evangelou, B.P., Wagner, G., 1989. Relationships between Cadmium, Zinc, Cd-peptide, and organic acid in tobacco suspension cells. Plant Physiology, 91:780-787.

[16] Lastra, O., Chueca, A., González, C., Lachica, M., Gorge, J.L., 1987. El cobre como nutriente de la planta. Anales Edafol. Agrobiol, 46:1005-1020.

[17] Leita, L., De Nobili, M., Cesco, S., 1996. Analysis of intercellular cadmium forms in roots and leaves of bush bean. Journal of Plant Nutrition, 19(3&4):527-533.

[18] Lidon, F.C., Ramalho, J.C., Henriques, F.S., 1993. Copper inhibition of rice photosynthesis. Journal of Plant Physiology, 142:12-17.

[19] Macnair, M.R., 1993. The genetics of metal tolerance in vascular plants. New Phytologist, 124:541-559.

[20] Maksymiec, W., Baszynski, T., 1999. The role of Ca2+ ions in modulating changes induced in bean plants by an excess of Cu2+ ions. Chlorophyll fluorescence measurements, Physiologial Plantarum, 105:562-568.

[21] Maksymiec, W., Bednara, J., Baszynski, T., 1995. Responses of runner plants to excess copper as a function of plant growth stages: effects on morphology and structure of primary leaves and their chloroplast ultrastructure. Photosynthetica, 31:427-435.

[22] Mullen, M.D., Wolf, D.C., Beveridge, T.J., Bailey, G.W., 1992. Sorption of heavy metala by soil fungi Aspergillus niger and Mucor ruoxii. Soil Biology and Biochemistry, 24:129-135.

[23] Nishizono, H., Watanabe, T., Orii, T., Suzuki, S., 1989. Suppression of inhibitory effects of copper on enzymatic activities by copper-binding substances from Athyrium yokoscene assayed in vitro. Plant Cell Physiology, 30(4):565-569.

[24] Rae, T.D., Schmidt, P.J., Pufahl, R.A., Culotta, V.C., O’Halloran, T.V., 1999. Undetectable intracellular free copper: the requirement of a copper chaperone for superoxide dismutase. Science, 284:805-808.

[25] Robson, A.D., Reuter, D.J., 1981. Diagnosis of Copper Deficiency and Toxicity. In: Loneragan, J.F., Robson, A.D., Graham, R.D. (Eds.), Copper in Soils and Plants. Academic Press, London, p.287-312.

[26] Song, J., Zhao, F.J., Luo, Y.M., McGrath, S.P., Zhang, H., 2004. Copper uptake by Elsholtzia splendens and Silene vulgaris and assessment of copper phytoavailability in contaminated soils. Environmental Pollution, 128:307-315.

[27] Tang, S.R., Wilke, B.M., Huang, C.Y., 1999. The uptake of copper by plants dominantly growing on copper mining spoils along the Yangtze River, the People’s Republic of China. Plant and Soil, 209:225-232.

[28] Wagner, G.J., Krotz, R.M., 1989. Perspectives on Cd and Zn Accumulation, Accomodation and Tolerance in Plant Cells: The Role of Cd-binding Peptide versus Other Mechanisms. In: Alan, R. (Ed.), Molecular Biology and Chemistry (Metal ion homeostasis). Liss, Inc., p.325-336.

[29] Westerhoff, H.V., 1985. Organization in cell soup. Nature, 318:106-108.

[30] Wissenmeier, A.H., Klotz, F., Horst, W.J., 1987. Aluminum induced callose synthesis in roots of soybean (Glycine max L.). Journal of Plant Physiology, 129:487-492.

[31] Yang, X.E., Shi, W.Y., Fu, C.X., Yang, M.J., 1998. Copper-hyperaccumulators of Chinese Native Plants Characteristics and Possible Use for Phytoremediation. Sustainable Agriculture for Food, Energy and Industry, James & James (Science Publishers) Ltd.

[32] Yang, M.J., Yang, X.E., Roemheld, V., 2002. Growth and nutrient composition of Elsholtzia splendens nakai under copper toxicity. Journal of Plant Nutrition, 25(7):1359-1375.

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2024 Journal of Zhejiang University-SCIENCE