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Received: 2004-06-25

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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.6 P.502-507

http://doi.org/10.1631/jzus.2005.B0502


Characteristics of fruit ripening in tomato mutant epi


Author(s):  WANG Zhong-feng, YING Tie-jin, BAO Bi-li, HUANG Xiao-dan

Affiliation(s):  School of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   tjying@hzcnc.com

Key Words:  Epinastic (epi) mutant, Ethylene overproduction, Ethylene signaling, Fruit ripening


WANG Zhong-feng, YING Tie-jin, BAO Bi-li, HUANG Xiao-dan. Characteristics of fruit ripening in tomato mutant epi[J]. Journal of Zhejiang University Science B, 2005, 6(6): 502-507.

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author="WANG Zhong-feng, YING Tie-jin, BAO Bi-li, HUANG Xiao-dan",
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Abstract: 
The characteristics of fruit ripening and expression of ripening-related genes were investigated in epi, an ethylene overproduction mutant of tomato (Lycopersicon esculentum Mill.). The epi produces apparently more ethylene than its wild type VFN8 at every stage of vegetative and fruit growth and ripening; compared to VFN8, the epi fruit showed higher CO2 evolution, faster descending of chlorophyll, slightly quicker increase of carotenoid and lycopene, and faster reduction in pericarp firmness during maturation and ripening; and the mRNAs of three ripening-related genes including E8, pTOM5 and pTOM6 were at higher levels in epi. The ripening-related characteristics changing of the fruit are consistent with the increase of ethylene production and ripening-related genes expression. These results suggest that epi mutation possibly did not affect the ethylene perception and signaling during fruit ripening, and that the modified characteristics of fruit ripening possibly resulted from the ethylene overproduction and increased expression of ripening-related genes.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1] Barry, C.S., Fox, E.A., Yen, H.C., Lee, S.H., Ying, T.J., Grierson, D., Giovannoni, J.J., 2001. Analysis of the ethylene response in the epinastic mutant of tomato. Plant Physiol., 127:58-66.

[2] Bird, C.R., Ray, J.A., Fletcher, J.D., Boniwell, J.M., Bird, A.S., Teulieres, C., Blain, I., Bramley, P.M., Schuch, W., 1991. Using antisense RNA to study gene function: inhibition of carotenoid biosynthesis in transgenic tomatoes. Bio. Technology, 9:635-639.

[3] Davies, B.H., 1976. Carotenoids. In: Goodwin, T.W. (Ed.), Chemistry and Biochemistry of Plant Pigments. Academic Press London, New York, San Francisco, 2:38-165.

[4] Deikman, J., Kline, R., Fischer, R.L., 1992. Organization of ripening and ethylene regulatory regions in a fruit-specific promoter from tomato (Lycopersicon esculentum). Plant Physiol., 100:2013-2017.

[5] Fujino, D.W., Burger, D.W., Yang, S.F., Bradford, K.J., 1988. Characterization of an ethylene overproducing mutant of tomato (Lycopersicon esculentum Mill. cultivar VFN8). Plant Physiol., 88:774-779.

[6] Fujino, D.W., Burger, D.W., Bradford, K.J., 1989. Ineffectiveness of ethylene biosynthetic and action inhibitors in phenotypically reverting the Epenastic mutant of tomato (Lycopersicon esculentum Mill.). J Plant Growth Regul., 8:53-61.

[7] Grierson, D., Maunders, M.J., Slater, A., Ray, J., Bird, C.R., Schuch, W., Holdsworth, G.A., Knapp, J.E., 1986. Gene expression during tomato ripening. Philos. Trans. R. Soc. Lond-Biol. Sci., 314:399-410.

[8] Guo, H., Ecker, J.R., 2004. The ethylene signaling pathway: New insights. Current Opinion in Plant Biology, 7:40-49.

[9] Jackman, R.L., Marangoni, A.G., Stanley, D.W., 1990. Measurement of tomato fruit firmness. HortiScience, 25:781-783.

[10] Kieber, J.J., Rothenberg, M., Roman, G., Feldmann, K.A., Ecker, J.R., 1993. CTR1, a negative regulator of the ethylene response pathway in Arabidopsis, encodes a member of the raf family of protein kinases. Cell, 72:427-441.

[11] Kirk, J.T.O., 1968. Studies on the dependence of chlorophyll synthesis on protein synthesis in Euglena gracilis together with a nomognam for determination of chlorophyll concentration. Planta, 78:200-207.

[12] Lashbrook, C.C., Gonzalez-Bosch, C., Bennett, A.B., 1994. Two divergent endo-b-1,4-glucanase genes exhibit overlapping expression in ripening fruit and abscising flowers. Plant Cell, 6:1485-1493.

[13] Lee, K.Y., Baden, C., Howie, W.J., Bedbrook, J., Dunsmuir, P., 1997. Post-transcriptional gene silencing of ACC synthase in tomato results from cytoplasmic RNA degradation. Plant J., 12:1127-1137.

[14] Lincoln, J.M., Cordes, S., Read, E., Fischer, R.L., 1987. Regulation of gene expression by ethylene during Lycopersicon esculentum (tomato) fruit development. Proc. Natl. Acad. Sci. USA, 84:2793-2797.

[15] Moctezuma, E., Smith, D.L., Gross, K.C., 2003. Effect of ethylene on mRNA abundance of three β-galactosidase genes in wild type and mutant tomato fruit. Postharvest Biology and Technology, 28:207-217.

[16] Tieman, D.M., Taylor, M.G., Ciardi, J.A., Klee, H.J., 2000. The tomato ethylene receptors NR and LeETR4 are negative regulators of the ethylene response and exhibit functional compensation within a multigene family. Proc Natl Acad Sci USA, 97:5663-5668.

[17] Tomes, M.L., 1963. Temperature inhibition of carotene synthesis in tomato. Bot. Gaz., 124:180-185.

[18] Ursin, V.M., 1987. Morphogenetic and Physiological Analysis of Two Developmental Mutant of Tomato (Lycopersicon Esculentum Mill.), Epinastics and Iageotropic. PhD dissertation, University of Califorlia, Davis.

[19] Ursin, V.M., Bradford, K.J., 1989. Auxin and ethylene regulation of petiole epinasty in two developmental mutants of tomato, diageotropic and epinastic. Plant Physiol., 90:1341-1346.

[20] Wang, Z.F., Ying, T.J., 2004. Phenotypes analysis of ethylene response in tomato mutant Epinastics. J. Plant Physiol. and Mol. Biol., 30:27-33 (in Chinese).

[21] Ying, T.J., Zeng, G.W., 1999. Differential expression of ACC synthase and ACC oxidase gene families in furit of Epinastics, a tomato mutant overproducing ethylene. J. Zhejiang University (Agricultural and life Sciences), 25:458-490 (in Chinese).

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barbara<babby_25_69@hotmail.com>

2010-09-30 09:31:05

muuy bueno el articulo

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