CLC number: X131.3
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
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FANG Zhao-hua, WEN Yue-zhong, LIU Wei-ping. Influence of kaolinite on chiral hydrolysis of methyl dichlorprop enantiomers[J]. Journal of Zhejiang University Science B, 2005, 6(10): 1028-1032.
@article{title="Influence of kaolinite on chiral hydrolysis of methyl dichlorprop enantiomers",
author="FANG Zhao-hua, WEN Yue-zhong, LIU Wei-ping",
journal="Journal of Zhejiang University Science B",
volume="6",
number="10",
pages="1028-1032",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B1028"
}
%0 Journal Article
%T Influence of kaolinite on chiral hydrolysis of methyl dichlorprop enantiomers
%A FANG Zhao-hua
%A WEN Yue-zhong
%A LIU Wei-ping
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 10
%P 1028-1032
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B1028
TY - JOUR
T1 - Influence of kaolinite on chiral hydrolysis of methyl dichlorprop enantiomers
A1 - FANG Zhao-hua
A1 - WEN Yue-zhong
A1 - LIU Wei-ping
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 10
SP - 1028
EP - 1032
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B1028
Abstract: The effect of kaolinite on the enzymatic chiral hydrolysis of methyl dichlorprop enantiomers ((R,S)-methyl-2-(2,4-dichlorophenoxy) propanoic acid, 2,4-DPM) was investigated using chiral gas chromatography. Compared with the control without kaolinite, the enantiomeric ratio (ER) increased from 1.35 to 8.33 and the residual ratio of 2,4-DPM decreased from 60.89% to 41.55% in the presence of kaolinite. kaolinite likely had emotion influence on lipase activity and its enantioselectivity. Moreover, the amount of kaolinite added was also found to be a sensitive factor affecting the enantioselective hydrolysis of 2,4-DPM. Fourier transform infrared (FTIR) spectroscopy studies of the interaction of lipase with kaolinite provided insight into the molecular structure of the complex and offered explanation of the effects of kaolinite on enzymatic hydrolysis of 2,4-DPM. Spectra showed that the effect of kaolinite on the hydrolysis of 2,4-DPM was affected by adsorption of lipase on kaolinite and changes of adsorbed lipase conformation, which led to the modified enantioselectivity.
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