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Received: 2005-10-29

Revision Accepted: 2006-02-20

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Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.4 P.320-323


Inactivation of infectious bursal disease virus by binary ethylenimine and formalin

Author(s):  Habib M., Hussain I., Fang W.H., Rajput Z.I., Yang Z.Z., Irshad H.

Affiliation(s):  Department of Veterinary Microbiology, University of Agriculture, Faisalabad 38040, Pakistan; more

Corresponding email(s):   mudasserhabib@yahoo.com

Key Words:  Infectious bursal disease virus (IBDV), Binary ethylenimine (BEI), Inactivation

Habib M., Hussain I., Fang W.H., Rajput Z.I., Yang Z.Z., Irshad H.. Inactivation of infectious bursal disease virus by binary ethylenimine and formalin[J]. Journal of Zhejiang University Science B, 2006, 7(4): 320-323.

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journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

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%T Inactivation of infectious bursal disease virus by binary ethylenimine and formalin
%A Habib M.
%A Hussain I.
%A Fang W.H.
%A Rajput Z.I.
%A Yang Z.Z.
%A Irshad H.
%J Journal of Zhejiang University SCIENCE B
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%D 2006
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0320

T1 - Inactivation of infectious bursal disease virus by binary ethylenimine and formalin
A1 - Habib M.
A1 - Hussain I.
A1 - Fang W.H.
A1 - Rajput Z.I.
A1 - Yang Z.Z.
A1 - Irshad H.
J0 - Journal of Zhejiang University Science B
VL - 7
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SP - 320
EP - 323
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2006.B0320

In this experiment conducted to study the inactivation dynamics of infectious bursal disease virus (IBDV) by binary ethylenimine (BEI) in comparison with formalin, IBDV was isolated from the bursa of infected chickens and its confirmation was done by agar gel precipitation test. Viral suspensions were subjected to inactivation with BEI and formalin for pre-set time intervals. BEI was employed at concentrations of 0.001 and 0.002 mol/L while formalin was used at 0.1% and 0.2%. Sampling was done at 6, 12, 24, 36 and 48 h of incubation and samples were tested for their inactivation status in 9-day-old embryonated eggs and 3-week-old broiler chickens. IBDV was completely inactivated by 0.001 and 0.002 mol/L BEI after 36 h of incubation at 37 °C, whereas formalin at 0.1% and 0.2% concentrations inactivated IBDV in 24 h.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1] Allan, W.H., Faragher, J.T., Cullen, G.A., 1972. Immunosuppression by the infectious bursal agent in chickens immunised against Newcastle disease. Vet. Rec., 90(18):511-512.

[2] Bahnemann, H.G., 1990. Inactivation of viral antigens for vaccine preparation with particular reference to the application of binary ethylenimine. Vaccine, 8(4):299-303.

[3] Barnes, H.J., Wheeler, J., Reed, D., 1982. Serological evidence of infectious bursal disease infection in Iowa turkeys. Avian Dis., 26:560-565.

[4] Blackburn, N.K., Besselaar, T.G., 1991. A study of the effect of chemical inactivants on the epitopes of Rift Valley fever virus glycoproteins using monoclonal antibodies. J. Virol. Methods, 33(3):367-374.

[5] Box, P., 1989. High maternal antibodies help chickens beat virulent virus. World Poultry, 53:17-19.

[6] Brown, F., 1995. Vaccination today and tomorrow. Biosci. Rep., 15(6):493-502.

[7] Buonavoglia, C., Iovane, G., Trani, L.D., Falcone, F., Orefice, L., Di Trani, L., 1988a. Evaluation of the immunogenicity in pigs guineapigs and chickens of an inactivated porcine parvovirus vaccine. Acta Medica Veterinaria, 34:445-452.

[8] Buonavoglia, C., Fioretti, A., Tollis, M., Menna, F., Papparella, V., 1988b. A preliminary vaccine potency trial of a Newcastle disease virus inactivated with binary ethylenimine. Vet. Res. Comm., 12(2-3):195-197.

[9] Dilovski, M., Tekerlekov, P., 1983. Comparative testing of the quality of foot-and-mouth disease vaccines prepared with different virus inactivators. Vet. Med. Nauki., 20(5-6):41-45.

[10] Helmboldt, C.F., Garner, E., 1964. Experimentally induced gumboro disease (IBA). Avian Dis., 8:561-575.

[11] Hitchner, S.B., 1970. Infectivity of infectious bursal disease virus for embryonating eggs. Poult. Sci., 49(2):511-516.

[12] ICTV (International Committee on Taxonomy of Virus), 2004. Infectious Bursal Disease Virus. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/

[13] Ismail, N.M., Saif, Y.M., Moorhead, P.D., 1988. Lack of pathogenicity of five serotype 2 infectious bursal disease viruses in chickens. Avian Dis., 32(4):757-759.

[14] Jackwood, D.J., Saif, Y.M., Hughes, J.H., 1982. Characteristics and serologic studies of two serotypes of infectious bursal disease virus in turkeys. Avian Dis., 26(4):871-882.

[15] Kibenge, F.S., Dhillon, A.S., Russell, R.G., 1988. Biochemistry and immunology of infectious bursal disease virus. Journal of General Virology, 69(Pt 8):1757-1775.

[16] King, D.J., 1991. Evaluation of different methods of inactivation of Newcastle disease virus and avian influenza virus in egg fluids and serum. Avian Dis., 35(3):505-514.

[17] Larghi, O.P., Nebel, A.E., 1980. Rabies virus inactivation by binary ethylenimine: new method for inactivated vaccine production. Journal of Clinical Microbiology, 11(2):120-122.

[18] Lukert, P.D., Saif, Y.M., 1991. Infectious Bursal Disease. In: Calnek, B.W., Barnes, H.J., Beard, C.W., Reid, W.M., Yoder, H.W.Jr, (Eds.), Diseases of Poultry. Iowa State University Press, Ames, p.648-663.

[19] Özel, M., Gelderblom, H., 1985. Capsid symmetry of viruses of the proposed birnavirus group. Arch. Virol., 84(3-4):149-161.

[20] Pyke, A.T., Phillips, D.A., Chuan, T.F., Smith, G.A., 2004. Sucrose density gradient centrifugation and cross-flow filtration methods for the production of arbovirus antigens inactivated by binary ethylenimine. BMC Microbiol., 4(1):3.

[21] Reddy, Y.K., Koteeswaren, A., Dorairajan, N., 1977. Pattern of infectious bursal disease in commercial white Leghorn chicken. Indian Vet. J., 74:1019-1021.

[22] Reed, L.J., Muench, H., 1938. A simple method of estimating fifty percent endpoints. Amer. J. Hyg., 27:493-497.

[23] Rosenberger, J.K., Cloud, S.S., Gelb, J., Odor, E., Dohms, J.E., 1985. Sentinel Bird Survey of Delmarva Broiler Flocks. Proc. 20th Natl. Meet. Poult. Health Condemn. Ocean City, MD, p.94-101.

[24] Soliman, I.M.A., El-Kabany, M.M.A., Kalad, M.A., Abdel-Baky, M.H., 1996. Immune response in horses vaccinated with a combined freeze dried vaccine and tetanus toxoid. Assiut. Vet. Med. J., 35(70):209-221.

[25] Stott, J.L., Osburn, B.I., Barber, T.L., 1979. The current status of research on an experimental inactivated bluetongue virus vaccine. Proc. Annu. Meet U S Anim. Health Assoc., (83):55-62.

[26] Sulochana, S., Lalithakunjamma, C.R., 1991. Infectious bursal disease in Kerala. Cheiron, 20:180-183.

[27] Zierenberg, K., Raue, R., Nieper, H., Islam, M.R., Eterradossi, N., Toquin, D., Müller, H., 2004. Generation of serotype 1/serotype 2 reassortant viruses of the infectious bursal disease virus and their investigation in vitro and in vivo. Virus Res., 105(1):23-34.

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