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Received: 2005-10-29

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Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.4 P.320-323


Inactivation of infectious bursal disease virus by binary ethylenimine and formalin

Author(s):  Habib M., Hussain I., Fang W.H., Rajput Z.I., Yang Z.Z., Irshad H.

Affiliation(s):  Department of Veterinary Microbiology, University of Agriculture, Faisalabad 38040, Pakistan; more

Corresponding email(s):   mudasserhabib@yahoo.com

Key Words:  Infectious bursal disease virus (IBDV), Binary ethylenimine (BEI), Inactivation

Habib M., Hussain I., Fang W.H., Rajput Z.I., Yang Z.Z., Irshad H.. Inactivation of infectious bursal disease virus by binary ethylenimine and formalin[J]. Journal of Zhejiang University Science B, 2006, 7(4): 320-323.

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publisher="Zhejiang University Press & Springer",

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%T Inactivation of infectious bursal disease virus by binary ethylenimine and formalin
%A Habib M.
%A Hussain I.
%A Fang W.H.
%A Rajput Z.I.
%A Yang Z.Z.
%A Irshad H.
%J Journal of Zhejiang University SCIENCE B
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%D 2006
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0320

T1 - Inactivation of infectious bursal disease virus by binary ethylenimine and formalin
A1 - Habib M.
A1 - Hussain I.
A1 - Fang W.H.
A1 - Rajput Z.I.
A1 - Yang Z.Z.
A1 - Irshad H.
J0 - Journal of Zhejiang University Science B
VL - 7
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SP - 320
EP - 323
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2006.B0320

In this experiment conducted to study the inactivation dynamics of infectious bursal disease virus (IBDV) by binary ethylenimine (BEI) in comparison with formalin, IBDV was isolated from the bursa of infected chickens and its confirmation was done by agar gel precipitation test. Viral suspensions were subjected to inactivation with BEI and formalin for pre-set time intervals. BEI was employed at concentrations of 0.001 and 0.002 mol/L while formalin was used at 0.1% and 0.2%. Sampling was done at 6, 12, 24, 36 and 48 h of incubation and samples were tested for their inactivation status in 9-day-old embryonated eggs and 3-week-old broiler chickens. IBDV was completely inactivated by 0.001 and 0.002 mol/L BEI after 36 h of incubation at 37 °C, whereas formalin at 0.1% and 0.2% concentrations inactivated IBDV in 24 h.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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