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Received: 2007-01-22

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Journal of Zhejiang University SCIENCE B 2007 Vol.8 No.5 P.302-306


Determination of fluorinated quinolone antibacterials by ion chromatography with fluorescence detection

Author(s):  ZHANG Yan-zhen, ZHANG Zheng-yi, ZHOU Yan-chun, LIU Li, ZHU Yan

Affiliation(s):  Department of Stomatology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China; more

Corresponding email(s):   zyz85@hotmail.com, zhuyan@zju.edu.cn

Key Words:  Fluorinated quinolone, Ion chromatography, Fluorescence detection

ZHANG Yan-zhen, ZHANG Zheng-yi, ZHOU Yan-chun, LIU Li, ZHU Yan. Determination of fluorinated quinolone antibacterials by ion chromatography with fluorescence detection[J]. Journal of Zhejiang University Science B, 2007, 8(5): 302-306.

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author="ZHANG Yan-zhen, ZHANG Zheng-yi, ZHOU Yan-chun, LIU Li, ZHU Yan",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Determination of fluorinated quinolone antibacterials by ion chromatography with fluorescence detection
%A ZHANG Yan-zhen
%A ZHANG Zheng-yi
%A ZHOU Yan-chun
%A ZHU Yan
%J Journal of Zhejiang University SCIENCE B
%V 8
%N 5
%P 302-306
%@ 1673-1581
%D 2007
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.B0302

T1 - Determination of fluorinated quinolone antibacterials by ion chromatography with fluorescence detection
A1 - ZHANG Yan-zhen
A1 - ZHANG Zheng-yi
A1 - ZHOU Yan-chun
A1 - LIU Li
A1 - ZHU Yan
J0 - Journal of Zhejiang University Science B
VL - 8
IS - 5
SP - 302
EP - 306
%@ 1673-1581
Y1 - 2007
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2007.B0302

For preparing fluorinated quinolone antibiotic medicine locally used in stomatology, simultaneous determination of norfloxacin, ciprofloxacin, and enoxacin was carried out by multiphase ion chromatography with fluorescence detection. Quinolone antibiotics were separated by Dionex OmniPac PAX-500 column with an eluent of 15 mmol/L H2SO4 and 35% methanol (v/v) at a flow-rate of 1.0 ml/min and detected with fluorescence with excitation and emission wave lengths of 347 nm and 420 nm respectively. The detection limits (S/N=3) of norfloxacin, ciprofloxacin and enoxacin were 50, 105 and 80 ng/ml respectively. The relative standard deviations of retention time, peak area and peak height were less than 1.1% and good linear relationship resulted. The developed method was applied to pharmaceutical formulations and biological fluids.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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