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On-line Access: 2022-06-16

Received: 2022-04-24

Revision Accepted: 2022-08-18

Crosschecked: 2022-09-16

Cited: 0

Clicked: 231

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Hongjuan WANG

https://orcid.org/0000-0002-5019-8305

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Journal of Zhejiang University SCIENCE B 2022 Vol.23 No.9 P.778-783

http://doi.org/10.1631/jzus.B2200233


Nanobubbles loaded with carbon quantum dots for ultrasonic fluorescence dual detection


Author(s):  Yankun ZHANG, Bingtao TANG, Yu XIN, Qiong WU, Lan LIU, Yunxia WANG, Kongxi ZHU, Guimei LIN, Hongjuan WANG

Affiliation(s):  Department of Gastroenterology, the Second Hospital of Shandong University, Jinan 250033, China; more

Corresponding email(s):   wanghongjuan2017@126.com

Key Words:  Liposome, Nanobubble, Ultrasound imaging, Carbon quantum dots


Yankun ZHANG, Bingtao TANG, Yu XIN, Qiong WU, Lan LIU, Yunxia WANG, Kongxi ZHU, Guimei LIN, Hongjuan WANG. Nanobubbles loaded with carbon quantum dots for ultrasonic fluorescence dual detection[J]. Journal of Zhejiang University Science B, 2022, 23(9): 778-783.

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author="Yankun ZHANG, Bingtao TANG, Yu XIN, Qiong WU, Lan LIU, Yunxia WANG, Kongxi ZHU, Guimei LIN, Hongjuan WANG",
journal="Journal of Zhejiang University Science B",
volume="23",
number="9",
pages="778-783",
year="2022",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2200233"
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%A Lan LIU
%A Yunxia WANG
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A1 - Yu XIN
A1 - Qiong WU
A1 - Lan LIU
A1 - Yunxia WANG
A1 - Kongxi ZHU
A1 - Guimei LIN
A1 - Hongjuan WANG
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DOI - 10.1631/jzus.B2200233


Abstract: 
To increase the efficiency and accuracy of clinical tumor detection, we explored multiple imaging by preparing carbon quantum dot (CQD)-loaded nanobubbles for ultrasonic fluorescence dual detection. In this experiment, we prepared 1,2-dioleoyl3-trimethylammonium-propane chloride (DOTAP) cationic liposomes using the film dispersion method and chose perfluoropentane as the core gas material of the nanobubbles. The nanobubbles were coupled with the negatively charged CQDs through the charge effect to prepare the testing agent for two-way diagnosis with ultrasound contrast and fluorescence detection. The formulation and preparation of the loaded CQD liposome nanobubbles were screened. In vivo experiments showed that nanobubbles can be enriched to the tumor site within 5 min, which enables clearer ultrasound imaging and is conducive to tumor detection. We expect CQD-loaded liposome (Lip-CQD) nanobubbles to become a new ultrasonic contrast agent for clinical applications that can provide a basis for early tumor diagnosis and thus earlier treatment.

负载碳量子点的纳米泡用于超声荧光双重检测

张艳坤2,唐炳涛3,辛玉1,吴琼1,刘岚1,王云霞1,朱孔锡1,林贵梅2,王红娟1
1山东大学第二医院消化科,中国济南市,250033
2山东大学药学院,中国济南市,250012
3齐鲁工业大学(山东省科学院)机械工程学院,中国济南市,250353
概要:为提高临床肿瘤检测效率和准确性,我们通过制备负载碳量子点(CQDs)的纳米泡进行超声荧光双重检测,探索多重成像。在本实验中,我们采用薄膜分散法制备了阳离子脂质体,并使用全氟戊烷作为纳米气泡的核心气体材料。通过电荷效应将纳米气泡与带负电荷的碳量子点耦合,制备出利用超声对比和荧光检测进行双向诊断的检测剂,并对负载碳量子点脂质体纳米气泡的配方和制备进行了筛选。当全氟戊烷用量为5 µL,胆固醇与(2,3-二油酰基-丙基)-三甲基氯化铵(DOTAP)的质量比为1:1,在超声功率为20%,时间为1 s的条件下,纳米泡的平均粒径为(224.6±2.4)nm,平均zeta电位为(46.2±1.3)mV。体内实验表明,纳米气泡可以在5分钟内富集到肿瘤部位,使超声成像更加清晰,有利于肿瘤的检测。载有CQDs的脂质体纳米泡有望成为一种新的临床应用的超声造影剂,可以为肿瘤诊断提供依据,并为肿瘤治疗提供机会。

关键词:脂质体;纳米气泡;超声成像;碳量子点

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Reference

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