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On-line Access: 2022-07-19

Received: 2021-11-29

Revision Accepted: 2022-02-09

Crosschecked: 2022-07-19

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Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Tao HU

https://orcid.org/0000-0002-4868-7150

Xiao LI

https://orcid.org/0000-0002-0133-0197

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Journal of Zhejiang University SCIENCE A 2022 Vol.23 No.7 P.579-586

http://doi.org/10.1631/jzus.A2100584


Free-standing MXene/chitosan/Cu2O electrode: an enzyme-free and efficient biosensor for simultaneous determination of glucose and cholesterol


Author(s):  Tao HU, Man ZHANG, Hui DONG, Tong LI, Xiao-bei ZANG, Xiao LI, Zhong-hua NI

Affiliation(s):  School of Mechanical Engineering, Southeast University, Nanjing 211189, China; more

Corresponding email(s):   lx2016@seu.edu.cn

Key Words: 


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Tao HU, Man ZHANG, Hui DONG, Tong LI, Xiao-bei ZANG, Xiao LI, Zhong-hua NI. Free-standing MXene/chitosan/Cu2O electrode: an enzyme-free and efficient biosensor for simultaneous determination of glucose and cholesterol[J]. Journal of Zhejiang University Science A, 2022, 23(7): 579-586.

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publisher="Zhejiang University Press & Springer",
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Abstract: 
The incidence of "three highs", referring to hyperglycemia, hypertension, and hyperlipidemia, has been increasing rapidly all over the world (Dey and Raj, 2013; Gao et al., 2019). These illnesses may be attributed to the perturbations of blood metabolites which are small molecules within biofluids (Patil et al., 2018). Among them, the presence of cholesterol and glucose in blood at abnormal levels highly increases the risk of cardiac and brain vascular diseases, and diabetes (Li et al., 2019). Furthermore, it has been confirmed that high glucose concentration is one of the main causes of atherosclerosis, by causing the accumulation of cholesterol in macrophages (Henry et al., 2002). Therefore, for early diagnosis of these diseases, the development of a low-cost, simple, and efficient strategy for simultaneous, accurate, and rapid detection of multiple metabolites is greatly needed for on-site disease monitoring in-home healthcare.

自支撑MXene/壳聚糖/Cu2O电极:一种高效的同时测定葡萄糖和胆固醇的无酶生物传感器

作者:胡涛1,2,张曼1,2,董慧1,2,李彤1,2,臧晓蓓3,李晓1,2,倪中华1,2
机构:1东南大学,机械工程学院,中国南京,2111892;2东南大学,江苏省微纳生物医学仪器设计与制造重点实验室,中国南京,2111893;3中国石油大学(华东),材料科学与工程学院,中国青岛,266580
目的:研制低成本、高精度的多种代谢物同时检测的生物传感器对医疗诊断具有重要意义。在本工作中,我们提出了一种基于MXene/壳聚糖(CTS)/Cu2O纳米复合材料的独立无酶电极,用于同时高精度测定葡萄糖和胆固醇。
创新点:1.利用MXene、CTS和Cu2O的协同作用,通过电位分离,实现对葡萄糖和胆固醇的无酶同时检测;2.优化检测范围,可用于检测人体血液样本。
方法:1.通过MXene、CTS和Cu2O纳米材料的协同作用形成有效的界面结,促进反应过程中的电荷转移,进而提高与待测物质的接触面积;2.分析电化学反应过程,构建电流信号与待测物浓度之间的关系,得到传感器的性能参数。
结论:1. MXene/CTS薄膜具有较高的比表面积,为离子的扩散提供了更多的通道,同时Cu2O纳米粒子可以提供丰富的金属活性边缘,促进了电荷转移,提高了反应活性。2. Cu2O纳米颗粒在胆固醇检测中发挥了重要作用,而同时作为导电基底和葡萄糖氧化剂的MXene,通过协同作用实现了在不同电位上同时无酶检测葡萄糖和胆固醇。3.在优化的电位范围内(?0.80-0.40 V),该传感器对葡萄糖和胆固醇具有良好的线性响应,灵敏度分别为60.295和215.71μA?L/(mmol?cm2),而检出限分别为52.4和49.8 μmol/L。4.通过对人血清样品的实时分析,验证了其良好的抗干扰能力和回收率(98.04%-102.94%),所以该传感器具有一定的临床应用前景。

关键词:无酶电极;多物质同时检测;胆固醇;葡萄糖;MXene

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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