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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.4 P.291-298

http://doi.org/10.1631/jzus.B0710470


Using oligonucleotide suspension arrays for laboratory identification of bacteria responsible for bacteremia


Author(s):  Xiao-li HOU, Han-liang JIANG, Qing-yi CAO, Li-ying ZHAO, Barbara J. CHANG, Zhi CHEN

Affiliation(s):  Institute of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China; more

Corresponding email(s):   chenzhi@zju.edu.cn

Key Words:  Oligonucleotide array, Bacteremia, 23S rRNA, Multiplexed detection


Xiao-li HOU, Han-liang JIANG, Qing-yi CAO, Li-ying ZHAO, Barbara J. CHANG, Zhi CHEN. Using oligonucleotide suspension arrays for laboratory identification of bacteria responsible for bacteremia[J]. Journal of Zhejiang University Science B, 2008, 9(4): 291-298.

@article{title="Using oligonucleotide suspension arrays for laboratory identification of bacteria responsible for bacteremia",
author="Xiao-li HOU, Han-liang JIANG, Qing-yi CAO, Li-ying ZHAO, Barbara J. CHANG, Zhi CHEN",
journal="Journal of Zhejiang University Science B",
volume="9",
number="4",
pages="291-298",
year="2008",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0710470"
}

%0 Journal Article
%T Using oligonucleotide suspension arrays for laboratory identification of bacteria responsible for bacteremia
%A Xiao-li HOU
%A Han-liang JIANG
%A Qing-yi CAO
%A Li-ying ZHAO
%A Barbara J. CHANG
%A Zhi CHEN
%J Journal of Zhejiang University SCIENCE B
%V 9
%N 4
%P 291-298
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0710470

TY - JOUR
T1 - Using oligonucleotide suspension arrays for laboratory identification of bacteria responsible for bacteremia
A1 - Xiao-li HOU
A1 - Han-liang JIANG
A1 - Qing-yi CAO
A1 - Li-ying ZHAO
A1 - Barbara J. CHANG
A1 - Zhi CHEN
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 4
SP - 291
EP - 298
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0710470


Abstract: 
The aim of this study was to develop and validate an oligonucleotide suspension array for rapid identification of 15 bacterial species responsible for bacteremia, particularly prevalent in Chinese hospitals. The multiplexed array, based on the QIAGEN LiquiChip Workstation, included 15 oligonucleotide probes which were covalently bound to different bead sets. PCR amplicons of a variable region of the bacterial 23S rRNA genes were hybridized to the bead-bound probes. Thirty-eight strains belonging to 15 species were correctly identified on the basis of their corresponding species-specific hybridization profiles. The results show that the suspension array, in a single assay, can differentiate isolates over a wide range of strains and species, and suggest the potential utility of suspension array system to clinical laboratory diagnosis.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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