CLC number: R587
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 0000-00-00
Cited: 1
Clicked: 6317
Xiao-zhi QIAO, Xian-feng WANG, Zhe-rong XU, Yun-mei YANG. Resistin does not down-regulate the transcription of insulin receptor promoter[J]. Journal of Zhejiang University Science B, 2008, 9(4): 313-318.
@article{title="Resistin does not down-regulate the transcription of insulin receptor promoter",
author="Xiao-zhi QIAO, Xian-feng WANG, Zhe-rong XU, Yun-mei YANG",
journal="Journal of Zhejiang University Science B",
volume="9",
number="4",
pages="313-318",
year="2008",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0710637"
}
%0 Journal Article
%T Resistin does not down-regulate the transcription of insulin receptor promoter
%A Xiao-zhi QIAO
%A Xian-feng WANG
%A Zhe-rong XU
%A Yun-mei YANG
%J Journal of Zhejiang University SCIENCE B
%V 9
%N 4
%P 313-318
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0710637
TY - JOUR
T1 - Resistin does not down-regulate the transcription of insulin receptor promoter
A1 - Xiao-zhi QIAO
A1 - Xian-feng WANG
A1 - Zhe-rong XU
A1 - Yun-mei YANG
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 4
SP - 313
EP - 318
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0710637
Abstract: Objective: To detect the effect of resistin on the transcription of insulin receptor promoter. Methods: luciferase reporter gene was fused downstream of human insulin receptor promoter and the enzymatic activity of luciferase was determined in the presence or absence of resistin. The resistin expressed with plasmid was stained with antibody against Myc tag which was in frame fused with resistin coding sequence, and then imaged with confocal microscopy. Results: The treatment of pIRP-LUC transfected cells with recombinant resistin did not result in significant difference in the enzymatic activity of luciferase compared to the untreated cells. Cell staining showed that green fluorescence could be observed in the cytoplasm, but not in the nucleus. Conclusion: The results suggest that the endogenous resistin may functionally locate in the cytoplasm, but does not enter the nucleus and not down-regulate the transcription of insulin receptor promoter.
[1] Adeghate, E., Schattner, P., Dunn, E., 2006. An update on the etiology and epidemiology of diabetes mellitus. Ann. N. Y. Acad. Sci., 1084(1):1-29.
[2] Al-Daghri, N., Chetty, R., McTernan, P.G., Al-Rubean, K., Al-Attas, O., Jones, A.F., Kumar, S., 2005. Serum resistin is associated with C-reactive protein and LDL cholesterol in type 2 diabetes and coronary artery disease in a Saudi population. Cardiovasc. Diabetol., 4(1):10.
[3] Al-Sari, I.E., Al-Quobaili, F.A., Kabalan, Y.M., 2007. Serum resistin levels in Syrian obese patients with diabetes mellitus type II. Saudi. Med. J., 28(12):1890-1894.
[4] Banerjee, R.R., Rangwala, S.M., Shapiro, J.S., Rich, A.S., Rhoades, B., Qi, Y., Wang, J., Rajala, M.W., Pocai, A., Scherer, P.E., et al., 2004. Regulation of fasted blood glucose by resistin. Science, 303(5661):1195-1198.
[5] Brown, J.E., Onyango, D.J., Dunmore, S.J., 2007. Resistin down-regulates insulin receptor expression, and modulates cell viability in rodent pancreatic beta-cells. FEBS Lett., 581(17):3273-3276.
[6] Fan, H.Q., Gu, N., Liu, F., Fei, L., Pan, X.Q., Guo, M., Chen, R.H., Guo, X.R., 2007. Prolonged exposure to resistin inhibits glucose uptake in rat skeletal muscles. Acta Pharmacol. Sin., 28(3):410-416.
[7] Fehmann, H.C., Heyn, J., 2002. Plasma resistin levels in patients with type 1 and type 2 diabetes mellitus and in healthy controls. Horm. Metab. Res., 34(11-12):671-673.
[8] Gerber, M., Boettner, A., Seidel, B., Lammert, A., Bar, J., Schuster, E., Thiery, J., Kiess, W., Kratzsch, J., 2005. Serum resistin levels of obese and lean children and adolescents: biochemical analysis and clinical relevance. J. Clin. Endocrinol. Metab., 90(8):4503-4509.
[9] Holcomb, I.N., Kabakoff, R.C., Chan, B., Baker, T.W., Gurney, A., Henzel, W., Nelson, C., Lowman, H.B., Wright, B.D., Skelton, N.J., et al., 2000. FIZZ1, a novel cysteine-rich secreted protein associated with pulmonary inflammation, defines a new gene family. EMBO J., 19(15):4046-4055.
[10] Kershaw, E.E., Flier, J.S., 2004. Adipose tissue as an endocrine organ. J. Clin. Endocrinol. Metab., 89(6):2548-2556.
[11] Kim, K.H., Lee, K., Moon, Y.S., Sul, H.S., 2001. A cysteine-rich adipose tissue-specific secretory factor inhibits adipocyte differentiation. J. Biol. Chem., 276(14):11252-11256.
[12] Liu, F., Guo, X.R., Gong, H.X., Ni, Y.H., Fei, L., Pan, X.Q., Guo, M., Chen, R.H., 2006. A resistin binding peptide selected by phage display inhibits 3T3-L1 preadipocyte differentiation. Chin. Med. J. (Engl.), 119(6):496-503.
[13] Liu, F., Yang, T., Wang, B., Zhang, M., Gu, N., Qiu, J., 2008. Resistin induces insulin resistance, but does not affect glucose output in rat-derived hepatocytes. Acta Pharmacol. Sin., 29(1):98-104.
[14] Minn, A.H., Patterson, N.B., Pack, S., Hoffmann, S.C., Gavrilova, O., Vinson, C., Harlan, D.M., Shalev, A., 2003. Resistin is expressed in pancreatic islets. Biochem. Biophys. Res. Commun., 310(2):641-645.
[15] Muse, E.D., Obici, S., Bhanot, S., Monia, B.P., McKay, R.A., Rajala, M.W., Scherer, P.E., Rossetti, L., 2004. Role of resistin in diet-induced hepatic insulin resistance. J. Clin. Invest., 114(2):232-239.
[16] Proietto, J., 2005. Mechanisms of insulin resistance caused by nutrient toxicity. Hepatol. Res., 33(2):87-91.
[17] Qiao, X.Z., Wang, X.F., Zhao, D.J., Xu, Z.R., Yu, L.D., Chen, Z., Yang, Y.M., 2007a. Assembly of full-length human resistin gene in vitro and construction of its eukaryotic expression vector. J. Zhejiang Univ. (Med. Ed.), 36(6):588-591 (in Chinese).
[18] Qiao, X.Z., Yang, Y.M., Xu, Z.R., Yang, L.A., 2007b. Relationship between resistin level in serum and acute coronary syndrome or stable angina pectoris J. Zhejiang Univ. Sci. B, 8(12):875-880.
[19] Rajala, M.W., Obici, S., Scherer, P.E., Rossetti, L., 2003. Adipose-derived resistin and gut-derived resistin-like molecule—beta selectively impair insulin action on glucose production. J. Clin. Invest., 111(2):225-230.
[20] Steppan, C.M., Bailey, S.T., Bhat, S., Brown, E.J., Banerjee, R.R., Wright, C.M., Patel, H.R., Ahima, R.S., Lazar, M.A., 2001a. The hormone resistin links obesity to diabetes. Nature, 409(6818):307-312.
[21] Steppan, C.M., Brown, E.J., Wright, C.M., Bhat, S., Banerjee, R.R., Dai, C.Y., Enders, G.H., Silberg, D.G., Wen, X., Wu, G.D., et al., 2001b. A family of tissue-specific resistin-like molecules. Proc. Natl. Acad. Sci. USA, 98(2):502-506.
[22] Tokuyama, Y., Osawa, H., Ishizuka, T., Onuma, H., Matsui, K., Egashira, T., Makino, H., Kanatsuka, A., 2007. Serum resistin level is associated with insulin sensitivity in Japanese patients with type 2 diabetes mellitus. Metabolism, 56(5):693-698.
[23] Ukkola, O., 2002. Resistin—a mediator of obesity-associated insulin resistance or an innocent bystander? Eur. J. Endocrinol., 147(5):571-574.
[24] Yagmur, E., Trautwein, C., Gressner, A.M., Tacke, F., 2006. Resistin serum levels are associated with insulin resistance, disease severity, clinical complications, and prognosis in patients with chronic liver diseases. Am. J. Gastroenterol., 101(6):1244-1252.
[25] Zhou, L., Li, Y., Xia, T., Feng, S., Chen, X., Yang, Z., 2006. Resistin overexpression impaired glucose tolerance in hepatocytes. Eur. Cytokine Netw., 17(3):189-195.
Open peer comments: Debate/Discuss/Question/Opinion
<1>