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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.4 P.313-318

http://doi.org/10.1631/jzus.B0710637


Resistin does not down-regulate the transcription of insulin receptor promoter


Author(s):  Xiao-zhi QIAO, Xian-feng WANG, Zhe-rong XU, Yun-mei YANG

Affiliation(s):  Department of Very Important Person, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China; more

Corresponding email(s):   qiao.xiaozhi@163.com, gbbf2000@sina.com

Key Words:  Resistin, Insulin receptor promoter, Luciferase reporter gene


Xiao-zhi QIAO, Xian-feng WANG, Zhe-rong XU, Yun-mei YANG. Resistin does not down-regulate the transcription of insulin receptor promoter[J]. Journal of Zhejiang University Science B, 2008, 9(4): 313-318.

@article{title="Resistin does not down-regulate the transcription of insulin receptor promoter",
author="Xiao-zhi QIAO, Xian-feng WANG, Zhe-rong XU, Yun-mei YANG",
journal="Journal of Zhejiang University Science B",
volume="9",
number="4",
pages="313-318",
year="2008",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0710637"
}

%0 Journal Article
%T Resistin does not down-regulate the transcription of insulin receptor promoter
%A Xiao-zhi QIAO
%A Xian-feng WANG
%A Zhe-rong XU
%A Yun-mei YANG
%J Journal of Zhejiang University SCIENCE B
%V 9
%N 4
%P 313-318
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0710637

TY - JOUR
T1 - Resistin does not down-regulate the transcription of insulin receptor promoter
A1 - Xiao-zhi QIAO
A1 - Xian-feng WANG
A1 - Zhe-rong XU
A1 - Yun-mei YANG
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 4
SP - 313
EP - 318
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0710637


Abstract: 
Objective: To detect the effect of resistin on the transcription of insulin receptor promoter. Methods: luciferase reporter gene was fused downstream of human insulin receptor promoter and the enzymatic activity of luciferase was determined in the presence or absence of resistin. The resistin expressed with plasmid was stained with antibody against Myc tag which was in frame fused with resistin coding sequence, and then imaged with confocal microscopy. Results: The treatment of pIRP-LUC transfected cells with recombinant resistin did not result in significant difference in the enzymatic activity of luciferase compared to the untreated cells. Cell staining showed that green fluorescence could be observed in the cytoplasm, but not in the nucleus. Conclusion: The results suggest that the endogenous resistin may functionally locate in the cytoplasm, but does not enter the nucleus and not down-regulate the transcription of insulin receptor promoter.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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