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CLC number: R739.41

On-line Access: 2015-09-05

Received: 2015-03-18

Revision Accepted: 2015-07-06

Crosschecked: 2015-08-18

Cited: 4

Clicked: 4426

Citations:  Bibtex RefMan EndNote GB/T7714


Xiao-min Hua


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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.9 P.757-762


DNA methylation level of promoter region of activating transcription factor 5 in glioma

Author(s):  Xiao-min Hua, Juan Wang, Dong-meng Qian, Jing-yi Song, Hao Chen, Xiu-li Zhu, Rui Zhou, Yu-dan Zhao, Xiu-zhi Zhou, Ling Li, Li Zhang, Xu-xia Song, Bin Wang

Affiliation(s):  1Department of Microbiology, Qingdao University Medical College, Qingdao 266071, China; more

Corresponding email(s):   wangbin532@126.com

Key Words:  DNA methylation, Activating transcription factor, Promoter region, Epigenetic, Glioma

Xiao-min Hua, Juan Wang, Dong-meng Qian, Jing-yi Song, Hao Chen, Xiu-li Zhu, Rui Zhou, Yu-dan Zhao, Xiu-zhi Zhou, Ling Li, Li Zhang, Xu-xia Song, Bin Wang. DNA methylation level of promoter region of activating transcription factor 5 in glioma[J]. Journal of Zhejiang University Science B, 2015, 16(9): 757-762.

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author="Xiao-min Hua, Juan Wang, Dong-meng Qian, Jing-yi Song, Hao Chen, Xiu-li Zhu, Rui Zhou, Yu-dan Zhao, Xiu-zhi Zhou, Ling Li, Li Zhang, Xu-xia Song, Bin Wang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T DNA methylation level of promoter region of activating transcription factor 5 in glioma
%A Xiao-min Hua
%A Juan Wang
%A Dong-meng Qian
%A Jing-yi Song
%A Hao Chen
%A Xiu-li Zhu
%A Rui Zhou
%A Yu-dan Zhao
%A Xiu-zhi Zhou
%A Ling Li
%A Li Zhang
%A Xu-xia Song
%A Bin Wang
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 9
%P 757-762
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500067

T1 - DNA methylation level of promoter region of activating transcription factor 5 in glioma
A1 - Xiao-min Hua
A1 - Juan Wang
A1 - Dong-meng Qian
A1 - Jing-yi Song
A1 - Hao Chen
A1 - Xiu-li Zhu
A1 - Rui Zhou
A1 - Yu-dan Zhao
A1 - Xiu-zhi Zhou
A1 - Ling Li
A1 - Li Zhang
A1 - Xu-xia Song
A1 - Bin Wang
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 9
SP - 757
EP - 762
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500067

Transcription factors, which represent an important class of proteins that play key roles in controlling cellular proliferation and cell cycle modulation, are attractive targets for cancer therapy. Previous researches have shown that the expression level of activating transcription factor 5 (ATF5) was frequently increased in glioma and its acetylation level was related to glioma. The purposes of this study were to explore the methylation level of ATF5 in clinical glioma tissues and to explore the effect of ATF5 methylation on the expression of ATF5 in glioma. Methylation of the promoter region of ATF5 was assayed by bisulfite-specific polymerase chain reaction (PCR) sequencing analysis in 35 cases of glioma and 5 normal tissues. Quantitative real-time PCR (qRT-PCR) was also performed to detect ATF5 mRNA expression in 35 cases of glioma and 5 normal tissues. Clinical data were collected from the patients and analyzed. The percentages of methylation of the ATF5 gene in the promoter region in healthy control, patients with well-differentiated glioma, and those with poorly differentiated glioma were 87.78%, 73.89%, and 47.70%, respectively. Analysis of the methylation status of the promoter region of the ATF5 gene showed a gradually decreased methylation level in poorly differentiated glioma, well-differentiated glioma, and normal tissues (P<0.05). There was also a significant difference between well-differentiated glioma and poorly differentiated glioma (P<0.05). ATF5 mRNA expression in glioma was significantly higher than that in the normal tissues (P<0.05). This study provides the first evidence that the methylation level of ATF5 decreased, and its mRNA expression was evidently up-regulated in glioma.


方法:收集35临床胶质瘤组织及5例急性脑外伤组织作为对照,应用亚硫酸盐测序技术检测ATF5的甲基化水平,并结合临床病理资料进行分析;实时荧光定量聚合酶链式反应(qRT-PCR)检测所有标本中ATF5 mRNA 的表达水平变化。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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