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Received: 2008-05-21

Revision Accepted: 2008-08-05

Crosschecked: 2008-11-26

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Journal of Zhejiang University SCIENCE B 2009 Vol.10 No.1 P.14-21


Chlorella vulgaris triggers apoptosis in hepatocarcinogenesis-induced rats

Author(s):  Emey Suhana MOHD AZAMAI, Suhaniza SULAIMAN, Shafina Hanim MOHD HABIB, Mee Lee LOOI, Srijit DAS, Nor Aini ABDUL HAMID, Wan Zurinah WAN NGAH, Yasmin Anum MOHD YUSOF

Affiliation(s):  Department of Biochemistry; more

Corresponding email(s):   anum@medic.ukm.my

Key Words:  Chlorella vulgaris (CV), Apoptosis, Bcl-2, Caspase 8, Liver cancer

Emey Suhana MOHD AZAMAI, Suhaniza SULAIMAN, Shafina Hanim MOHD HABIB, Mee Lee LOOI, Srijit DAS, Nor Aini ABDUL HAMID, Wan Zurinah WAN NGAH, Yasmin Anum MOHD YUSOF. Chlorella vulgaris triggers apoptosis in hepatocarcinogenesis-induced rats[J]. Journal of Zhejiang University Science B, 2009, 10(1): 14-21.

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author="Emey Suhana MOHD AZAMAI, Suhaniza SULAIMAN, Shafina Hanim MOHD HABIB, Mee Lee LOOI, Srijit DAS, Nor Aini ABDUL HAMID, Wan Zurinah WAN NGAH, Yasmin Anum MOHD YUSOF",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

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%T Chlorella vulgaris triggers apoptosis in hepatocarcinogenesis-induced rats
%A Emey Suhana MOHD AZAMAI
%A Suhaniza SULAIMAN
%A Shafina Hanim MOHD HABIB
%A Mee Lee LOOI
%A Srijit DAS
%A Wan Zurinah WAN NGAH
%A Yasmin Anum MOHD YUSOF
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820168

T1 - Chlorella vulgaris triggers apoptosis in hepatocarcinogenesis-induced rats
A1 - Emey Suhana MOHD AZAMAI
A1 - Suhaniza SULAIMAN
A1 - Shafina Hanim MOHD HABIB
A1 - Mee Lee LOOI
A1 - Srijit DAS
A1 - Wan Zurinah WAN NGAH
A1 - Yasmin Anum MOHD YUSOF
J0 - Journal of Zhejiang University Science B
VL - 10
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SP - 14
EP - 21
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Y1 - 2009
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B0820168

Chlorella vulgaris (CV) has been reported to have antioxidant and anticancer properties. We evaluated the effect of CV on apoptotic regulator protein expression in liver cancer-induced rats. Male Wistar rats (200~250 g) were divided into eight groups: control group (normal diet), CDE group (choline deficient diet supplemented with ethionine in drinking water to induce hepatocarcinogenesis), CV groups with three different doses of CV (50, 150, and 300 mg/kg body weight), and CDE groups treated with different doses of CV (50, 150, and 300 mg/kg body weight). Rats were sacrificed at various weeks and liver tissues were embedded in paraffin blocks for immunohistochemistry studies. CV, at increasing doses, decreased the expression of anti-apoptotic protein, bcl-2, but increased the expression of pro-apoptotic protein, caspase 8, in CDE rats, which was correlated with decreased hepatoctyes proliferation and increased apoptosis as determined by bromodeoxy-uridine (BrdU) labeling and terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling (TUNEL) assay, respectively. Our study shows that CV has definite chemopreventive effect by inducing apoptosis via decreasing the expression of bcl-2 and increasing the expression of caspase 8 in hepatocarcinogenesis-induced rats.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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