Full Text:   <2368>

CLC number: Q78

On-line Access: 

Received: 2008-07-05

Revision Accepted: 2008-10-11

Crosschecked: 2008-10-28

Cited: 3

Clicked: 5137

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.12 P.944-952


Functional characterization of a potassium transporter gene NrHAK1 in Nicotiana rustica

Author(s):  Zhao-kui GUO, Qian YANG, Xiu-qing WAN, Pei-qiang YAN

Affiliation(s):  Department of Life Sciences and Engineering, Harbin Institute of Technology, Harbin 150001, China; more

Corresponding email(s):   yangq@hit.edu.cn

Key Words:  Functional characterization, K+ transporter, Nicotiana rustica

Zhao-kui GUO, Qian YANG, Xiu-qing WAN, Pei-qiang YAN. Functional characterization of a potassium transporter gene NrHAK1 in Nicotiana rustica[J]. Journal of Zhejiang University Science B, 2008, 9(12): 944-952.

@article{title="Functional characterization of a potassium transporter gene NrHAK1 in Nicotiana rustica",
author="Zhao-kui GUO, Qian YANG, Xiu-qing WAN, Pei-qiang YAN",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Functional characterization of a potassium transporter gene NrHAK1 in Nicotiana rustica
%A Zhao-kui GUO
%A Qian YANG
%A Xiu-qing WAN
%A Pei-qiang YAN
%J Journal of Zhejiang University SCIENCE B
%V 9
%N 12
%P 944-952
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820209

T1 - Functional characterization of a potassium transporter gene NrHAK1 in Nicotiana rustica
A1 - Zhao-kui GUO
A1 - Qian YANG
A1 - Xiu-qing WAN
A1 - Pei-qiang YAN
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 12
SP - 944
EP - 952
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820209

The purpose of this study is to investigate the function of a novel potassium transporter gene (NrHAK1) isolated from Nicotiana rustica roots using yeast complement and real-time PCR technique. The complementary DNA (cDNA) of NrHAK1, 2 488 bp long, contains an open reading frame (ORF) of 2 334 bp encoding a protein of 777 amino acids (87.6 kDa) with 12 predicted transmembrane domains. The NrHAK1 protein shows a high sequence similarity to those of high-affinity potassium transporters in Mesembryanthemum, Phytolacca acinosa, Arabidopsis thaliana, and so on. We found that the NrHAK1 gene could complement the yeast-mutant defect in K+ uptake. Among several tissues surveyed, the expression level of NrHAK1 was most abundant in the root tip and was up-regulated when exposed to potassium starvation. Moreover, the transcript accumulation was significantly reduced by adding 5 mmol/L NH4+ to the solution. These results suggest that NrHAK1 plays an important role in potassium absorption in N. rustica.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1] Ahn, S.J., Shin, R., Schachtman, D., 2004. Expression of KT/KUP genes in Arabidopsis and the role of root hairs in K+ uptake. Plant Physiol., 134(3):1135-1145.

[2] Banuelos, M.A., Garciadeblas, B., Cubero, B., Rodriguez-Navarro, A., 2002. Inventory and functional characterization of the HAK potassium transporters of rice. Plant Physiol., 130(2):784-795.

[3] Epstein, E., Rains, D.W., Elzam, O.E., 1963. Resolution of dual mechanisms of potassium absorption by barley roots. Proc. Natl. Acad. Sci., 49(5):684-692.

[4] Fernando, M., Kulpa, J., Siddiqi, Y.M., Glass, A.D.M., 1990. Potassium-dependent changes in the expression of membrane-associated proteins in barley roots. Plant Physiol., 92(4):1128-1132.

[5] Fu, H.H., Luan, S., 1998. AtKUP1: a dual-affinity K+ transporter from Arabidopsis. The Plant Cell, 10(1):63-73.

[6] Fulgenzi, F.R., Peralta, M.L., Mangano, S., Danna,C.H., Vallejo, A.J., Puigdomenech, P., Santa-María, G.E., 2008. The ionic environment controls the contribution of the barley HvHAK1 transporter to potassium acquisition. Plant Physiol., 147(1):252-262.

[7] Gierth, M., Maser, P., Schroeder, J.I., 2005. The potassium transporter AtHAK5 functions in K+ deprivation-induced high-affinity K+ uptake and AKT1 K+ channel contribution to K+ uptake kinetics in Arabidopsis roots. Plant Physiol., 137(3):1105-1114.

[8] Gietz, D.R., Schiestl, R.H., Willems, A.R., Woods, R.A., 1995. Studies on the transformation of intact yeast cells by the LiAc/SS-DNA/PEG procedure. Yeast, 11(4):355-360.

[9] Glass, A.D.M., Dunlop, J., 1978. The influence of potassium content on the kinetics of potassium influx into excised ryegrass and barley roots. Planta, 141(1):117-119.

[10] Hirsch, R.E., Lewis, B.D., Spalding, E.P., Sussman, M.R., 1998. A role for the AKT1 potassium channel in plant nutrition. Science, 280(5365):918-921.

[11] Kim, E.J., Kwak, J.M., Uozumi, N., Schroeder, J.I., 1998. AtKUP1: an Arabidopsis gene encoding high affinity potassium transport activity. The Plant Cell, 10(1):51-62.

[12] Langer, K., Ache, P., Geiger, D., 2002. Poplar potassium transporters capable of controlling K+ homeostasis and K+-dependent xylogenesis. The Plant Journal, 32(6):997-1009.

[13] Maathuis, F.J., Filatov, V., Herzyk, P., Krijger, G., Axelsen, K.B., Chen, S., Green, B.J., Yi, L., Madagan, K.L., Sanchez-Fernandez, R., et al., 2003. Transcriptome analysis of root transporters reveals participation of multiple gene families in the response to cation stress. The Plant Journal, 35(6):675-692.

[14] Martínez-Cordero, M.A., Martínez, V., Rubio, F., 2004. Cloning and functional characterization of the high-affinity K+ transporter HAK1 of pepper. Plant Molecular Biology, 56(3):413-421.

[15] Martínez-Cordero, M.A., Martinez, V., Rubio, F., 2005. High-affinity K+ uptake in pepper plants. Journal of Experimental Botany, 56(416):1553-1562.

[16] Nieves-Cordones, M., Martínez-Cordero, A.M., Martínez, V., Rubio, F., 2007. An NH4+-sensitive component dominates high-affinity K+ uptake in tomato plants. Plant Science, 172(2):273-280.

[17] Pilot, G., Gaymard, F., Mouline, K., 2003. Regulated expression of Arabidopsis shaker K+ channel genes involved in K+ uptake and distribution in the plant. Plant Molecular Biology, 51(5):773-787.

[18] Qi, Z., Hampton, C.R., Shin, R., Barkla, B.J., White, P.J., Schachtman, D.P., 2008. The high affinity K+ transporter AtHAK5 plays a physiological role in planta at very low K+ concentrations and provides a caesium uptake pathway in Arabidopsis. Journal of Experimental Botany, 59(3):595-607.

[19] Quintero, F.J., Blatt, M.R., 1997. A new family of K+ transporters from Arabidopsis that are conserved across phyla. FEBS Letters, 415(2):206-211.

[20] Rigas, S., Debrosses, G., Haralampidis, K., Vicente-Agullo, F., Feldmann, K.A., Grabov, A., Dolan, L., Hatzopoulos, P., 2001. TRH1 encodes a potassium transporter required for tip growth in Arabidopsis root hairs. The Plant Cell, 13(1):139-151.

[21] Rodríguez-Navarro, A., 2000. Potassium transport in fungi and plants. Biochim Biophys Acta, 1469(1):1-30.

[22] Rodríuez-Navarro, A., Rubio, F., 2006. High-affinity potassium and sodium transport systems in plants. Journal of Experimental Botany, 57(5):1149-1160.

[23] Rubio, F., Santa-María, G.E., Rodriguez-Navarro, A., 2000. Cloning of Arabidopsis and barley cDNAs encoding HAK potassium transporters in root and shoot cells. Physiologia Plantarum, 109(1):34-43.

[24] Rufty, T.W., Jackson, W.A., Raper, D.C., 1982. Inhibition of nitrate assimilation in roots in the presence of ammonium: the moderating influence of potassium. Journal of Experimental Botany, 33(6):1122-1137.

[25] Santa-María, G.E., Rubio, F., Dubcovsky, J., Rodríguez-Navarro, A., 1997. The HAK1 gene of barley belongs to a large gene family and encodes a high-affinity potassium transporter. The Plant Cell, 9(12):2281-2289.

[26] Santa-María, G.E., Danna, C.H., Czibener, C., 2000. High-affinity potassium transport in barley roots: ammonium-sensitive and -insensitive pathways. Plant Physiol., 123(1):297-306.

[27] Schleyer, M., Bakker, E.P., 1993. Nucleotide sequence and 3′-end deletion studies indicate that the K+-uptake protein kup from Escherichia coli is composed of a hydrophobic core linked to a large and partially essential hydrophilic C terminus. J. Bacteriol., 175(21):6925-6931.

[28] Senn, M.E., Rubio, F., Banuelos, M.A., Rodriguez-Navarro, A., 2001. Comparative functional features of plant potassium HvHAK1 and HvHAK2 transporters. Journal of Biological Chemistry, 276(48):44563-44569.

[29] Su, H., Golldack, D., Zhao, C., Bohnert, H.J., 2002. The expression of HAK-type K+ transporters is regulated in response to salinity stress in common ice plant. Plant Physiol., 129(4):1482-1493.

[30] Wang, Y.H., Garvin, D.F., Kochian, L.V., 2002. Rapid induction of regulatory and transporter genes in response to phosphorus, potassium, and iron deficiencies in tomato roots. Evidence for cross talk and root/rhizosphere-mediated signals. Plant Physiol., 130(3):1361-1370.

[31] Wang, Z., Zhang, X., Fedida, D., 2000. Regulation of transient Na+ conductance by intra- and extracellular K+ in the human delayed rectifier K+ channel Kv1.5. The Journal of Physiology, 523(3):575-591.

Open peer comments: Debate/Discuss/Question/Opinion


Please provide your name, email address and a comment

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2022 Journal of Zhejiang University-SCIENCE