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Received: 2008-07-22

Revision Accepted: 2008-11-19

Crosschecked: 2008-11-19

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Journal of Zhejiang University SCIENCE B 2009 Vol.10 No.1 P.22-28


RNA interference against interleukin-5 attenuates airway inflammation and hyperresponsiveness in an asthma model

Author(s):  Shao-xing CHEN, Feng-ying HUANG, Guang-hong TAN, Cai-chun WANG, Yong-hao HUANG, Hua WANG, Song-lin ZHOU, Fan CHEN, Ying-ying LIN, Jun-bao LIU

Affiliation(s):  Hainan Provincial Key Laboratory of Tropical Medicine, Hainan Medical College, Haikou 571101, China; more

Corresponding email(s):   scihai@126.com

Key Words:  Interleukin-5 (IL-5), Small interfering RNA (siRNA), Airway hyperresponsiveness (AHR), Allergy, Lentivirus

Shao-xing CHEN, Feng-ying HUANG, Guang-hong TAN, Cai-chun WANG, Yong-hao HUANG, Hua WANG, Song-lin ZHOU, Fan CHEN, Ying-ying LIN, Jun-bao LIU. RNA interference against interleukin-5 attenuates airway inflammation and hyperresponsiveness in an asthma model[J]. Journal of Zhejiang University Science B, 2009, 10(1): 22-28.

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author="Shao-xing CHEN, Feng-ying HUANG, Guang-hong TAN, Cai-chun WANG, Yong-hao HUANG, Hua WANG, Song-lin ZHOU, Fan CHEN, Ying-ying LIN, Jun-bao LIU",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T RNA interference against interleukin-5 attenuates airway inflammation and hyperresponsiveness in an asthma model
%A Shao-xing CHEN
%A Feng-ying HUANG
%A Guang-hong TAN
%A Cai-chun WANG
%A Yong-hao HUANG
%A Song-lin ZHOU
%A Ying-ying LIN
%A Jun-bao LIU
%J Journal of Zhejiang University SCIENCE B
%V 10
%N 1
%P 22-28
%@ 1673-1581
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820226

T1 - RNA interference against interleukin-5 attenuates airway inflammation and hyperresponsiveness in an asthma model
A1 - Shao-xing CHEN
A1 - Feng-ying HUANG
A1 - Guang-hong TAN
A1 - Cai-chun WANG
A1 - Yong-hao HUANG
A1 - Hua WANG
A1 - Song-lin ZHOU
A1 - Fan CHEN
A1 - Ying-ying LIN
A1 - Jun-bao LIU
J0 - Journal of Zhejiang University Science B
VL - 10
IS - 1
SP - 22
EP - 28
%@ 1673-1581
Y1 - 2009
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820226

interleukin-5 (IL-5) accompanies the development of airway inflammation and hyperresponsiveness through the activation of eosinophils. Therefore, interference of IL-5 expression in lung tissue seems to be an accepted approach in asthma therapy. In this study, we designed a small interfering RNA (siRNA) to inhibit the expression of IL-5. The siRNAs against IL-5 were constructed in a lentivirus expressing system, and 1.5×106 IFU (inclusion-forming unit) lentiviruses were administered intratracheally to ovalbumin (OVA)-sensitized murine asthmatic models. Our results show that lentivirus-delivered siRNA against IL-5 efficiently inhibited the IL-5 messenger ribonucleic acid (mRNA) expression and significantly attenuated the inflammation in lung tissue. Significant decrease of eosinophils and inflammatory cells were found in peripheral blood, bronchoalveolar lavage fluid (BALF), and lung tissue. In addition, significant inhibition of airway hyperresponsiveness (AHR) was found in the mice treated with siRNA against IL-5. These observations demonstrate that siRNA delivered by means of the lentivirus system is possibly an efficacious therapeutic approach for asthma.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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