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CLC number: R562.2

On-line Access: 2019-09-06

Received: 2019-04-11

Revision Accepted: 2019-05-23

Crosschecked: 2019-08-08

Cited: 0

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Citations:  Bibtex RefMan EndNote GB/T7714


Xue-Jun Sun


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Journal of Zhejiang University SCIENCE B 2019 Vol.20 No.10 P.828-837


Protective effects of methane-rich saline on mice with allergic asthma by inhibiting inflammatory response, oxidative stress and apoptosis

Author(s):  Ning Zhang, Hong-Tao Lu, Rong-Jia Zhang, Xue-Jun Sun

Affiliation(s):  Department of Naval Aeromedicine, Naval Medical University, Shanghai 200433, China

Corresponding email(s):   sunxjk@hotmail.com

Key Words:  Asthma, Methane-rich saline, Antioxidation, Anti-inflammation, Anti-apoptotic

Ning Zhang, Hong-Tao Lu, Rong-Jia Zhang, Xue-Jun Sun. Protective effects of methane-rich saline on mice with allergic asthma by inhibiting inflammatory response, oxidative stress and apoptosis[J]. Journal of Zhejiang University Science B, 2019, 20(10): 828-837.

@article{title="Protective effects of methane-rich saline on mice with allergic asthma by inhibiting inflammatory response, oxidative stress and apoptosis",
author="Ning Zhang, Hong-Tao Lu, Rong-Jia Zhang, Xue-Jun Sun",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Protective effects of methane-rich saline on mice with allergic asthma by inhibiting inflammatory response, oxidative stress and apoptosis
%A Ning Zhang
%A Hong-Tao Lu
%A Rong-Jia Zhang
%A Xue-Jun Sun
%J Journal of Zhejiang University SCIENCE B
%V 20
%N 10
%P 828-837
%@ 1673-1581
%D 2019
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1900195

T1 - Protective effects of methane-rich saline on mice with allergic asthma by inhibiting inflammatory response, oxidative stress and apoptosis
A1 - Ning Zhang
A1 - Hong-Tao Lu
A1 - Rong-Jia Zhang
A1 - Xue-Jun Sun
J0 - Journal of Zhejiang University Science B
VL - 20
IS - 10
SP - 828
EP - 837
%@ 1673-1581
Y1 - 2019
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1900195

Background: asthma is a common cause of breathing difficulty in children and adults, and is characterized by chronic airway inflammation that is poorly controlled by available treatments. This results in severe disability and applies a huge burden to the public health system. Methane has been demonstrated to function as a therapeutic agent in many diseases. The aim of the present study was to explore the effect of methane-rich saline (MRS) on the pathophysiology of a mouse model of asthma and its underlying mechanism. Methods: A murine model of ovalbumin (OVA)-induced allergic asthma was applied in this study. Mice were divided into three groups: a control group, an OVA group, and OVA-induced asthmatic mice treated with MRS as the third group. Lung resistance index (RI) and dynamic compliance (Cdyn) were measured to determine airway hyper-responsiveness (AHR). Haematoxylin and eosin (H&E) staining was performed and scored to show histopathological changes. Cell counts of bronchoalveolar lavage fluid (BALF) were recorded. Cytokines interleukin (IL)-4, IL-5, IL-13, tumor necrosis factor α (TNF-α), and C-X-C motif chemokine ligand 15 (CXCL15) from BALF and serum were measured by enzyme-linked immunosorbent assay (ELISA). The oxidative stress indexes, including malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), myeloperoxidase (MPO), and 8-hydroxydeoxyguanosine (8-OHdG), were determined using commercial kits. Apoptosis was evaluated by western blot, quantitative real-time polymerase chain reaction (qRT-PCR), and biochemical examination. Results: MRS administration reversed the OVA-induced AHR, attenuated the pathological inflammatory infiltration, and decreased the cytokines IL-4, IL-5, IL-13, TNF-α, and CXCL15 in serum and BALF. Moreover, following MRS administration, the oxidative stress was alleviated as indicated by decreased MDA, MPO, and 8-OHdG, and elevated SOD and GSH. In addition, MRS exhibited an anti-apoptotic effect in this model, protecting epithelial cells from damage. Conclusions: Methane improves pulmonary function and decreases infiltrative inflammatory cells in the allergic asthmatic mouse model. This may be associated with its anti-inflammatory, antioxidative, and anti-apoptotic properties.




Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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