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On-line Access: 2023-02-05

Received: 2022-07-01

Revision Accepted: 2022-09-04

Crosschecked: 2023-02-09

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Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Jingquan DONG

https://orcid.org/0000-0001-9696-9681

Zibo DONG

https://orcid.org/0000-0003-1944-2645

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Journal of Zhejiang University SCIENCE B 2023 Vol.24 No.2 P.185-190

http://doi.org/10.1631/jzus.B2200340


Liensinine attenuates inflammation and oxidative stress in spleen tissue in an LPS-induced mouse sepsis model


Author(s):  Hanyu WANG, Yuanhao YANG, Xiao ZHANG, Yan WANG, Hui FAN, Jinfeng SHI, Xuelian TAN, Baoshi XU, Jingchao QIANG, Enzhuang PAN, Mingyi CHU, Zibo DONG, Jingquan DONG

Affiliation(s):  Jiangsu Key Laboratory of Marine Bioresources and Environment, Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, College of Pharmacy, Jiangsu Ocean University, Lianyungang 222005, China; more

Corresponding email(s):   dongzibo999@163.com, 2018000029@jou.edu.cn

Key Words:  Sepsis, Inflammatory, Oxidative stress, Spleen, Liensinine


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Hanyu WANG, Yuanhao YANG, Xiao ZHANG, Yan WANG, Hui FAN, Jinfeng SHI, Xuelian TAN, Baoshi XU, Jingchao QIANG, Enzhuang PAN, Mingyi CHU, Zibo DONG, Jingquan DONG. Liensinine attenuates inflammation and oxidative stress in spleen tissue in an LPS-induced mouse sepsis model[J]. Journal of Zhejiang University Science B, 2023, 24(2): 185-190.

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author="Hanyu WANG, Yuanhao YANG, Xiao ZHANG, Yan WANG, Hui FAN, Jinfeng SHI, Xuelian TAN, Baoshi XU, Jingchao QIANG, Enzhuang PAN, Mingyi CHU, Zibo DONG, Jingquan DONG",
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publisher="Zhejiang University Press & Springer",
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Abstract: 
sepsis is a complex syndrome caused by multiple pathogens and involves multiple organ failure, particularly spleen dysfunction. In 2017, the worldwide incidence was 48.9 million sepsis cases and 11 million sepsis-related deaths were reported (Rudd et al., 2020). Inflammation, oxidative stress, and apoptosis are the most common pathologies seen in sepsis. liensinine (LIE) is a bisbenzylisoquinoline-type alkaloid extracted from the seed embryo of Nelumbo nucifera. Lotus seed hearts have high content of LIE which mainly has antihypertensive and antiarrhythmic pharmacological effects. It can exert anti-carcinogenic activity by regulating cell, inflammation, and apoptosis signaling pathways (Manogaran et al., 2019). However, its protective effect from sepsis-induced spleen damage is unknown. In this research, we established a mouse sepsis model induced by lipopolysaccharide (LPS) and investigated the protective effects of LIE on sepsis spleen injury in terms of inflammatory response, oxidative stress, and apoptosis.

莲心碱可减轻LPS诱导的小鼠脓毒症模型中脾脏组织炎症和氧化应激反应

王晗毓1, 杨沅浩2, 张潇1, 王彦2, 樊慧1, 石锦峰1, 谭雪莲1, 徐宝石1, 强静超1, 潘恩壮1,储铭仪1, 董自波1, 董井泉1
1江苏省海洋生物资源与环境重点实验室, 江苏省海洋生物产业技术协同创新中心, 江苏省海洋药物化合物筛选重点实验室, 江苏海洋大学药学院, 中国连云港市, 222005
2连云港市第二人民医院风湿免疫科, 中国连云港市, 222000
概要:本研究基于脂多糖(LPS)诱导的小鼠脓毒症模型,探讨莲心碱(LIE)对脓毒症中脾损伤的潜在保护作用。脓毒症常伴有炎症、氧化应激和细胞凋亡,并将导致身体器官功能障碍,对脾脏损伤尤甚。将30只小鼠随机分为5组(n=6):对照组、LPS (10 mg/kg)、LIE(10 mg/kg)+LPS、LIE (20 mg/kg)+LPS和LIE (40 mg/kg)+LPS。脾脏损伤通过苏木精-伊红染色(H&E)确定;采用定量聚合酶链反应(qPCR)检测脾脏组织中肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)、IL-10、IL-1β和一氧化氮合酶(iNOS)的转录水平。同时对包括丙二醛(MDA)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)在内的氧化应激指标进行测定;采用TUNEL法检测细胞凋亡水平。结果显示,LIE可减轻脾脏组织病理学损伤并抑制细胞凋亡,显著降低促炎因子TNF-α、IL-6、IL-1β和iNOS的转录水平,并增加抗炎因子IL-10的表达。此外,LIE预处理后可降低MDA脂质过氧化指标,增强CAT、SOD和GSH-Px的抗氧化活性。综上所述,LIE具有潜在的抗氧化和抗炎能力,可减轻脾脏在脓毒症中的损伤。

关键词:脓毒症; 炎症反应; 氧化应激; 脾脏; 莲心碱

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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