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CLC number: R541

On-line Access: 2010-04-28

Received: 2009-11-16

Revision Accepted: 2010-03-15

Crosschecked: 2010-04-13

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Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.5 P.350-356

http://doi.org/10.1631/jzus.B0900369


Overexpression of the steroidogenic acute regulatory protein increases the expression of ATP-binding cassette transporters in microvascular endothelial cells (bEnd.3)


Author(s):  Yan-xia Ning, Shun-lin Ren, Feng-di Zhao, Lian-hua Yin

Affiliation(s):  Department of Physiology and Pathophysiology, Shanghai Medical College, Fudan University, Shanghai 200032, China, Department of Medicine, Virginia Commonwealth University, Richmond, Virginia 23298, USA, Veterans Affairs Medical Center, Richmond, Virginia 23249, USA

Corresponding email(s):   ningyx@fudan.edu.cn, lhyin@shmu.edu.cn

Key Words:  Steroidogenic acute regulatory protein (StAR), Endothelial cells, Cholesterol, Adenosine triphosphate (ATP)-binding cassette transporter A1 (ABCA1), ATP-binding cassette transporter G1 (ABCG1), bEnd.3


Yan-xia Ning, Shun-lin Ren, Feng-di Zhao, Lian-hua Yin. Overexpression of the steroidogenic acute regulatory protein increases the expression of ATP-binding cassette transporters in microvascular endothelial cells (bEnd.3)[J]. Journal of Zhejiang University Science B, 2010, 11(5): 350-356.

@article{title="Overexpression of the steroidogenic acute regulatory protein increases the expression of ATP-binding cassette transporters in microvascular endothelial cells (bEnd.3)",
author="Yan-xia Ning, Shun-lin Ren, Feng-di Zhao, Lian-hua Yin",
journal="Journal of Zhejiang University Science B",
volume="11",
number="5",
pages="350-356",
year="2010",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0900369"
}

%0 Journal Article
%T Overexpression of the steroidogenic acute regulatory protein increases the expression of ATP-binding cassette transporters in microvascular endothelial cells (bEnd.3)
%A Yan-xia Ning
%A Shun-lin Ren
%A Feng-di Zhao
%A Lian-hua Yin
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 5
%P 350-356
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0900369

TY - JOUR
T1 - Overexpression of the steroidogenic acute regulatory protein increases the expression of ATP-binding cassette transporters in microvascular endothelial cells (bEnd.3)
A1 - Yan-xia Ning
A1 - Shun-lin Ren
A1 - Feng-di Zhao
A1 - Lian-hua Yin
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 5
SP - 350
EP - 356
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0900369


Abstract: 
Objective: To determine the effect of steroidogenic acute regulatory protein (StAR) overexpression on the levels of adenosine triphosphate (ATP)-binding cassette transporter A1 (ABCA1) and ATP-binding cassette transporter G1 (ABCG1) in an endothelial cell line (bEnd.3). Methods: The StAR gene was induced in bEnd.3 cells with adenovirus infection. The infection efficiency was detected by fluorescence activated cell sorter (FACS) and fluorescence microscopy. The expressions of StAR gene and protein levels were detected by real-time polymerase chain reaction (PCR) and Western blot. The gene and protein levels of ABCA1 and ABCG1 were detected by real-time PCR and Western blot after StAR overexpression. Results: The result shows that StAR was successfully overexpressed in bEnd.3 cells by adenovirus infection. The mRNA and protein expressions of ABCA1 and ABCG1 were greatly increased by StAR overexpression in bEnd.3 cells. Conclusion: Overexpression of StAR increases ABCA1 and ABCG1 expressions in endothelial cells.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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