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CLC number: Q949.96

On-line Access: 2012-06-29

Received: 2011-01-17

Revision Accepted: 2011-11-30

Crosschecked: 2012-09-23

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Journal of Zhejiang University SCIENCE B 2012 Vol.13 No.10 P.783-790


Acute and subacute oral toxicity of Litsea elliptica Blume essential oil in rats

Author(s):  Siti Balkis Budin, Seri Masran Siti Nor Ain, Baharuddin Omar, Izatus Shima Taib, Othman Hidayatulfathi

Affiliation(s):  Biomedical Science Programme, School of Diagnostic and Applied Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, 50300 Jalan Raja Muda Abdul Aziz, Kuala Lumpur, Malaysia; more

Corresponding email(s):   hida@medic.ukm.my

Key Words:  Litsea elliptica, Acute toxicity, Subacute toxicity, Median lethal dose (LD50), Natural insecticide, Non-observed-adversed-effect level (NOAEL)

Siti Balkis Budin, Seri Masran Siti Nor Ain, Baharuddin Omar, Izatus Shima Taib, Othman Hidayatulfathi. Acute and subacute oral toxicity of Litsea elliptica Blume essential oil in rats[J]. Journal of Zhejiang University Science B, 2012, 13(10): 783-790.

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author="Siti Balkis Budin, Seri Masran Siti Nor Ain, Baharuddin Omar, Izatus Shima Taib, Othman Hidayatulfathi",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Acute and subacute oral toxicity of Litsea elliptica Blume essential oil in rats
%A Siti Balkis Budin
%A Seri Masran Siti Nor Ain
%A Baharuddin Omar
%A Izatus Shima Taib
%A Othman Hidayatulfathi
%J Journal of Zhejiang University SCIENCE B
%V 13
%N 10
%P 783-790
%@ 1673-1581
%D 2012
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1100021

T1 - Acute and subacute oral toxicity of Litsea elliptica Blume essential oil in rats
A1 - Siti Balkis Budin
A1 - Seri Masran Siti Nor Ain
A1 - Baharuddin Omar
A1 - Izatus Shima Taib
A1 - Othman Hidayatulfathi
J0 - Journal of Zhejiang University Science B
VL - 13
IS - 10
SP - 783
EP - 790
%@ 1673-1581
Y1 - 2012
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1100021

Litsea elliptica Blume has been traditionally used to treat headache, fever, and stomach ulcer, and has also been used as an insect repellent. The acute and subacute toxicities of L. elliptica essential oil were evaluated orally by gavage in female Sprague-Dawley rats. For the acute toxicity study, L. elliptica essential oil was administered in doses from 500 to 4000 mg/kg (single dose), and in the subacute toxicity test, the following doses were used: 125, 250, and 500 mg/kg, for 28 consecutive days. In the acute toxicity study, L. elliptica essential oil caused dose-dependent adverse behaviours and mortality. The median lethal dose value was 3488.86 mg/kg and the acute non-observed-adversed-effect level value was found to be 500 mg/kg. The subacute toxicity study of L. elliptica essential oil did not reveal alterations in body weight, and food and water consumptions. The haematological and biochemical analyses did not show significant differences between control and treated groups in most of the parameters examined, except for the hemoglobin, mean cell hemoglobin concentration, mean cell volume, mean cell hemoglobin, serum albumin, and serum sodium. However, these differences were still within the normal range. No abnormalities or histopathological changes were observed in the liver, pancreatic islet of Langerhans, and renal glomerulous and tubular cells of all treated groups. In conclusion, L. elliptica essential oil can be classified in the U group, which is defined as a group unlikely to present an acute hazard according to World Health Organization (WHO) classification.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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