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Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.5 P.366-377


A microarray analysis of early activated pathways in concanavalin A-induced hepatitis

Author(s):  Qing-yi Cao, Feng Chen, Jie Li, Shan-shan Wu, Jing Wang, Zhi Chen

Affiliation(s):  State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China

Corresponding email(s):   zju.zhichen@gmail.com

Key Words:  Concanavalin A, Fulminant hepatitis, Microarray, Expression profile, Pathway analysis

Qing-yi Cao, Feng Chen, Jie Li, Shan-shan Wu, Jing Wang, Zhi Chen. A microarray analysis of early activated pathways in concanavalin A-induced hepatitis[J]. Journal of Zhejiang University Science B, 2010, 11(5): 366-377.

@article{title="A microarray analysis of early activated pathways in concanavalin A-induced hepatitis",
author="Qing-yi Cao, Feng Chen, Jie Li, Shan-shan Wu, Jing Wang, Zhi Chen",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T A microarray analysis of early activated pathways in concanavalin A-induced hepatitis
%A Qing-yi Cao
%A Feng Chen
%A Jie Li
%A Shan-shan Wu
%A Jing Wang
%A Zhi Chen
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 5
%P 366-377
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000020

T1 - A microarray analysis of early activated pathways in concanavalin A-induced hepatitis
A1 - Qing-yi Cao
A1 - Feng Chen
A1 - Jie Li
A1 - Shan-shan Wu
A1 - Jing Wang
A1 - Zhi Chen
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 5
SP - 366
EP - 377
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000020

Objective: To explore the mechanisms of fulminant hepatitis (FH) in the early stages, and to determine the critical pathways in its initiation and progression. Methods: Twelve BALB/c mice were divided into four groups: one group left as negative control and sacrificed immediately after injection of phosphate-buffered saline (PBS), and another three groups with concanavalin A (Con A) administration sacrificed at 1, 3, and 6 h after injection. Affymetrix GeneChip® Mouse 430 2.0 Array was employed to evaluate the expression profile of each of the 12 samples. Further analysis was done on the microarray data to extract the genes that were differentially expressed. Enrichment analysis was carried out to determine relevant pathways within which regulated genes were significantly enriched. Results: A total of 393, 8354 and 11 344 differentially expressed genes were found, respectively, at three time points. During 0–1 h and 1–3 h, most of the pathways enriched with regulated genes were related to immune response and inflammation, among which Toll-like receptor (TLR) signaling and mitogen-activated protein kinase (MAPK) signaling appeared during both phases, while cytokine-cytokine receptor interaction, apoptosis, T cell receptor signaling, and natural killer (NK) cell-mediated cytotoxicity pathways emerged during the second phase. Pathways found to be significant during 3–6 h were mostly related to metabolic processes. Conclusion: The TLR signaling pathway dominates the early responses of Con A-induced FH in mice. It stimulates the production of type I cytokines, therefore recruiting and activating T/NK cells. Activated T/NK cells exert their cytotoxicity on hepatocytes through inducing death receptor-intermediated apoptosis, resulting in liver injury.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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