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Received: 2009-11-03

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Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.3 P.221-226

http://doi.org/10.1631/jzus.B0900351


Proteomic analysis of differentially expressed proteins in mice with concanavalin A-induced hepatitis


Author(s):  Xu-fei Tan, Feng Chen, Shan-shan Wu, Yu Shi, Dong-cheng Liu, Zhi Chen

Affiliation(s):  State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China

Corresponding email(s):   zju.zhichen@gmail.com

Key Words:  Concanavalin A (Con A), Liver injury, Protein, Two-dimensional electrophoresis (2-DE)


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Xu-fei Tan, Feng Chen, Shan-shan Wu, Yu Shi, Dong-cheng Liu, Zhi Chen. Proteomic analysis of differentially expressed proteins in mice with concanavalin A-induced hepatitis[J]. Journal of Zhejiang University Science B, 2010, 11(3): 221-226.

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author="Xu-fei Tan, Feng Chen, Shan-shan Wu, Yu Shi, Dong-cheng Liu, Zhi Chen",
journal="Journal of Zhejiang University Science B",
volume="11",
number="3",
pages="221-226",
year="2010",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0900351"
}

%0 Journal Article
%T Proteomic analysis of differentially expressed proteins in mice with concanavalin A-induced hepatitis
%A Xu-fei Tan
%A Feng Chen
%A Shan-shan Wu
%A Yu Shi
%A Dong-cheng Liu
%A Zhi Chen
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 3
%P 221-226
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0900351

TY - JOUR
T1 - Proteomic analysis of differentially expressed proteins in mice with concanavalin A-induced hepatitis
A1 - Xu-fei Tan
A1 - Feng Chen
A1 - Shan-shan Wu
A1 - Yu Shi
A1 - Dong-cheng Liu
A1 - Zhi Chen
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 3
SP - 221
EP - 226
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0900351


Abstract: 
Objective: To find new protein biomarkers for the detection and evaluation of liver injury and to analyze the relationship between such proteins and disease progression in concanavalin A (Con A)-induced hepatitis. Methods: Twenty-five mice were randomly divided into five groups: an untreated group, a control group injected with phosphate buffered saline (PBS), and groups with Con A-induced hepatitis evaluated at 1, 3 and 6 h. Two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) were used to identify differences in protein expression among groups. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to verify the results. Results: In mice with Con A-induced hepatitis, expression levels of four proteins were increased: RIKEN, fructose bisphosphatase 1 (fbp1), ketohexokinase (khk), and Chain A of class pi glutathione S-transferase. Changes in fbp1 and khk were confirmed by qRT-PCR. Conclusion: Levels of two proteins, fbp1 and khk, are clearly up-regulated in mice with Con A-induced hepatitis.

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