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On-line Access: 2010-09-08

Received: 2010-02-22

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Crosschecked: 2010-08-09

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Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.9 P.719-727


Effect of interrupted endogenous BMP/Smad signaling on growth and steroidogenesis of porcine granulosa cells

Author(s):  Wei Wang, Li Wang, Xin-xiu Li, Xia Chen, Hai-yan Zhang, Yu He, Jing-jing Wang, Yong-yan Zhao, Bao-le Zhang, Yin-xue Xu

Affiliation(s):  College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

Corresponding email(s):   xuyinxue@njau.edu.cn

Key Words:  Porcine granulosa cells, Bone morphogenetic protein (BMP)/Smad, Smad4, RNA interference (RNAi), Growth, Steroidogenesis

Wei Wang, Li Wang, Xin-xiu Li, Xia Chen, Hai-yan Zhang, Yu He, Jing-jing Wang, Yong-yan Zhao, Bao-le Zhang, Yin-xue Xu. Effect of interrupted endogenous BMP/Smad signaling on growth and steroidogenesis of porcine granulosa cells[J]. Journal of Zhejiang University Science B, 2010, 11(9): 719-727.

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author="Wei Wang, Li Wang, Xin-xiu Li, Xia Chen, Hai-yan Zhang, Yu He, Jing-jing Wang, Yong-yan Zhao, Bao-le Zhang, Yin-xue Xu",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Effect of interrupted endogenous BMP/Smad signaling on growth and steroidogenesis of porcine granulosa cells
%A Wei Wang
%A Li Wang
%A Xin-xiu Li
%A Xia Chen
%A Hai-yan Zhang
%A Yu He
%A Jing-jing Wang
%A Yong-yan Zhao
%A Bao-le Zhang
%A Yin-xue Xu
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 9
%P 719-727
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000079

T1 - Effect of interrupted endogenous BMP/Smad signaling on growth and steroidogenesis of porcine granulosa cells
A1 - Wei Wang
A1 - Li Wang
A1 - Xin-xiu Li
A1 - Xia Chen
A1 - Hai-yan Zhang
A1 - Yu He
A1 - Jing-jing Wang
A1 - Yong-yan Zhao
A1 - Bao-le Zhang
A1 - Yin-xue Xu
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 9
SP - 719
EP - 727
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000079

Bone morphogenetic proteins (BMPs) play a critical role in the growth and steroidogenesis of granulosa cells (GCs). BMP signals act through membrane-bound heteromeric serine/threonine kinase receptors. Upon ligand binding, BMPs activate intracellular Smad proteins and regulate growth and apoptosis in various cell types. The objective of this study was to demonstrate the effects of BMP/Smad signal on growth and steroidogenesis of porcine GCs. A strategy of RNA interference (RNAi)-mediated ‘gene silencing’ of smad4, a core molecule mediating the intracellular BMP/Smad signal transduction pathways, was used to interrupt endogenous BMP/Smad signaling. Results indicate that smad4-small interfering RNA (siRNA) caused specific inhibition of smad4 mRNA and protein expression after transfection. Interrupted endogenous BMP/Smad signaling significantly inhibited growth, and induced apoptosis of porcine GCs, while decreasing estradiol production. In addition, interrupted BMP/Smad signaling significantly (P<0.05) changed the expression of Cyclin D2, CDK4, Bcl-2, and Cyp19a1. These findings provide new insights into how BMP/Smad signaling regulates the growth and steroidogenesis of porcine GCs.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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