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CLC number: Q253

On-line Access: 2011-11-04

Received: 2011-01-18

Revision Accepted: 2011-07-27

Crosschecked: 2011-10-12

Cited: 7

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Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.11 P.875-883


Effect of epidermal growth factor on follicle-stimulating hormone-induced proliferation of granulosa cells from chicken prehierarchical follicles

Author(s):  Jin-xing Lin, Yu-dong Jia, Cai-qiao Zhang

Affiliation(s):  Key Laboratory of Animal Epidemic Etiology & Immunological Prevention of the Ministry of Agriculture, Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China

Corresponding email(s):   cqzhang@zju.edu.cn

Key Words:  Epidermal growth factor, Follicle-stimulating hormone, Granulosa cell, Proliferation, Chicken

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Jin-xing Lin, Yu-dong Jia, Cai-qiao Zhang. Effect of epidermal growth factor on follicle-stimulating hormone-induced proliferation of granulosa cells from chicken prehierarchical follicles[J]. Journal of Zhejiang University Science B, 2011, 12(11): 875-883.

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author="Jin-xing Lin, Yu-dong Jia, Cai-qiao Zhang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Effect of epidermal growth factor on follicle-stimulating hormone-induced proliferation of granulosa cells from chicken prehierarchical follicles
%A Jin-xing Lin
%A Yu-dong Jia
%A Cai-qiao Zhang
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 11
%P 875-883
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1100023

T1 - Effect of epidermal growth factor on follicle-stimulating hormone-induced proliferation of granulosa cells from chicken prehierarchical follicles
A1 - Jin-xing Lin
A1 - Yu-dong Jia
A1 - Cai-qiao Zhang
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 11
SP - 875
EP - 883
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1100023

The development of ovarian follicular cells is controlled by multiple circulating and local hormones and factors, including follicle-stimulating hormone (FSH) and epidermal growth factor (EGF). In this study, the stage-specific effect of EGF on FSH-induced proliferation of granulosa cells was evaluated in the ovarian follicles of egg-laying chickens. Results showed that EGF and its receptor (EGFR) mRNAs displayed a high expression in granulosa cells from the prehierarchical follicles, including the large white follicle (LWF) and small yellow follicle (SYF), and thereafter the expression decreased markedly to the stage of the largest preovulatory follicle. SYF represents a turning point of EGF/EGFR mRNA expression during follicle selection. Subsequently the granulosa cells from SYF were cultured to reveal the mediation of EGF in FSH action. Cell proliferation was remarkably increased by treatment with either EGF or FSH (0.1–100 ng/ml). This result was confirmed by elevated proliferating cell nuclear antigen (PCNA) expression and decreased cell apoptosis. Furthermore, EGF-induced cell proliferation was accompanied by increased mRNA expressions of EGFR, FSH receptor, and the cell cycle-regulating genes (cyclins D1 and E1, cyclin-dependent kinases 2 and 6) as well as decreased expression of luteinizing hormone receptor mRNA. However, the EGF or FSH-elicited effect was reversed by simultaneous treatment with an EGFR inhibitor AG1478. In conclusion, EGF and EGFR expressions manifested stage-specific changes during follicular development and EGF mediated FSH-induced cell proliferation and retarded cell differentiation in the prehierarchical follicles. These expressions thus stimulated follicular growth before selection in the egg-laying chicken.

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