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CLC number: Q96; Q51

On-line Access: 2011-02-04

Received: 2010-04-28

Revision Accepted: 2010-07-26

Crosschecked: 2011-01-07

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Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.2 P.93-102


Proteome changes in the plasma of Pieris rapae parasitized by the endoparasitoid wasp Pteromalus puparum

Author(s):  Jia-ying Zhu, Qi Fang, Gong-yin Ye, Cui Hu

Affiliation(s):  State Key Laboratory of Rice Biology and Key Laboratory of Molecular Biology of Crop Pathology and Insects of Ministry of Agriculture, Institute of Insect Sciences, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   chu@zju.edu.cn

Key Words:  Proteomics, Plasma, Parasitism, Gene cloning, Pteromalus puparum, Pieris rapae

Jia-ying Zhu, Qi Fang, Gong-yin Ye, Cui Hu. Proteome changes in the plasma of Pieris rapae parasitized by the endoparasitoid wasp Pteromalus puparum[J]. Journal of Zhejiang University Science B, 2011, 12(2): 93-102.

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author="Jia-ying Zhu, Qi Fang, Gong-yin Ye, Cui Hu",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Proteome changes in the plasma of Pieris rapae parasitized by the endoparasitoid wasp Pteromalus puparum
%A Jia-ying Zhu
%A Qi Fang
%A Gong-yin Ye
%A Cui Hu
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 2
%P 93-102
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000158

T1 - Proteome changes in the plasma of Pieris rapae parasitized by the endoparasitoid wasp Pteromalus puparum
A1 - Jia-ying Zhu
A1 - Qi Fang
A1 - Gong-yin Ye
A1 - Cui Hu
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 2
SP - 93
EP - 102
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000158

parasitism by the endoparasitoid wasp Pteromalus puparum causes alterations in the plasma proteins of Pieris rapae. Analysis of plasma proteins using a proteomic approach showed that seven proteins were differentially expressed in the host pupae after 24-h parasitism. They were masquerade-like serine proteinase homolog (MSPH), enolase (Eno), bilin-binding protein (BBP), imaginal disc growth factor (IDGF), ornithine decarboxylase (ODC), cellular retinoic acid binding protein (CRABP), and one unknown function protein. The full length cDNA sequences of MSPH, Eno, and BBP were successfully cloned using rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR). Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis indicated that the transcript levels of MSPH and BBP in the fat bodies of host pupae were inducible in response to the parasitism and their variations were consistent with translational changes of these genes after parasitism, while the transcript levels of Eno and IDGF were not affected by parasitism. This study will contribute to the better understanding of the molecular bases of parasitoid-induced host alterations associated with innate immune responses, detoxification, and energy metabolism.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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