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CLC number: R979.1

On-line Access: 2011-07-04

Received: 2010-06-09

Revision Accepted: 2010-12-03

Crosschecked: 2011-05-31

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Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.7 P.534-544


Ds-echinoside A, a new triterpene glycoside derived from sea cucumber, exhibits antimetastatic activity via the inhibition of NF-κB-dependent MMP-9 and VEGF expressions

Author(s):  Qin Zhao, Zhi-dong Liu, Yong Xue, Jing-feng Wang, Hui Li, Qing-juan Tang, Yu-ming Wang, Ping Dong, Chang-hu Xue

Affiliation(s):  College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China

Corresponding email(s):   jfwang@ouc.edu.cn, xuech@ouc.edu.cn

Key Words:  Triterpene glycoside, Ds-echinoside A (DSEA), Metastasis, Angiogenesis, Nuclear factor-κ, B (NF-κ, B), Matrix metalloproteinase-9 (MMP-9), Vascular endothelial growth factor (VEGF)

Qin Zhao, Zhi-dong Liu, Yong Xue, Jing-feng Wang, Hui Li, Qing-juan Tang, Yu-ming Wang, Ping Dong, Chang-hu Xue. Ds-echinoside A, a new triterpene glycoside derived from sea cucumber, exhibits antimetastatic activity via the inhibition of NF-κB-dependent MMP-9 and VEGF expressions[J]. Journal of Zhejiang University Science B, 2011, 12(7): 534-544.

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author="Qin Zhao, Zhi-dong Liu, Yong Xue, Jing-feng Wang, Hui Li, Qing-juan Tang, Yu-ming Wang, Ping Dong, Chang-hu Xue",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

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%T Ds-echinoside A, a new triterpene glycoside derived from sea cucumber, exhibits antimetastatic activity via the inhibition of NF-κB-dependent MMP-9 and VEGF expressions
%A Qin Zhao
%A Zhi-dong Liu
%A Yong Xue
%A Jing-feng Wang
%A Hui Li
%A Qing-juan Tang
%A Yu-ming Wang
%A Ping Dong
%A Chang-hu Xue
%J Journal of Zhejiang University SCIENCE B
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%P 534-544
%@ 1673-1581
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000217

T1 - Ds-echinoside A, a new triterpene glycoside derived from sea cucumber, exhibits antimetastatic activity via the inhibition of NF-κB-dependent MMP-9 and VEGF expressions
A1 - Qin Zhao
A1 - Zhi-dong Liu
A1 - Yong Xue
A1 - Jing-feng Wang
A1 - Hui Li
A1 - Qing-juan Tang
A1 - Yu-ming Wang
A1 - Ping Dong
A1 - Chang-hu Xue
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 7
SP - 534
EP - 544
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000217

ds-echinoside A (DSEA), a non-sulfated triterpene glycoside, was isolated from the sea cucumber Pearsonothuria graeffei. In vitro and in vivo investigations were conducted on the effects of DSEA on tumor cell adhesion, migration, invasion, and angiogenesis. In this study, we found that DSEA inhibited the proliferation of human hepatocellular liver carcinoma cells Hep G2, with a half-maximal inhibitory concentration (IC50) of 2.65 μmol/L, and suppressed Hep G2 cell adhesion, migration, and invasion in a dose-dependent manner. DSEA also reduced tube formation of human endothelial cells ECV-304 on matrigel in vitro and attenuated neovascularization in the chick embryo chorioallantoic membrane (CAM) assay in vivo. Immunocytochemical analysis revealed that DSEA significantly decreased the expression of matrix metalloproteinase-9 (MMP-9), which plays an important role in the degradation of basement membrane in tumor metastasis and angiogenesis. DSEA also increased the protein expression level of tissue inhibitor of metalloproteinase-1 (TIMP-1), an important regulator of MMP-9 activation. From the results of Western blotting, the expressions of nuclear factor-kappa b (NF-κ;b) and vascular endothelial growth factor (VEGF) were found to be remarkably reduced by DSEA. These findings suggest that DSEA exhibits a significant anti-metastatic activity through the specific inhibition of NF-κB-dependent MMP-9 and VEGF expressions.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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