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On-line Access: 2011-06-07

Received: 2010-09-13

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Crosschecked: 2011-05-09

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Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.6 P.419-427


Differences in dinucleotide frequencies of thermophilic genes encoding water soluble and membrane proteins

Author(s):  Hiroshi Nakashima, Yuka Kuroda

Affiliation(s):  Department of Clinical Laboratory Science, Graduate Course of Medical Science and Technology, School of Health Sciences, Kanazawa University, 5-11-80 Kodatsuno, Kanazawa 920-0942, Japan

Corresponding email(s):   naka@kenroku.kanazawa-u.ac.jp

Key Words:  Water soluble and membrane proteins, Purine/pyrimidine dimers, Thermophilic and mesophilic species, Dinucleotide frequencies

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Hiroshi Nakashima, Yuka Kuroda. Differences in dinucleotide frequencies of thermophilic genes encoding water soluble and membrane proteins[J]. Journal of Zhejiang University Science B, 2011, 12(6): 419-427.

@article{title="Differences in dinucleotide frequencies of thermophilic genes encoding water soluble and membrane proteins",
author="Hiroshi Nakashima, Yuka Kuroda",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Differences in dinucleotide frequencies of thermophilic genes encoding water soluble and membrane proteins
%A Hiroshi Nakashima
%A Yuka Kuroda
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 6
%P 419-427
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000331

T1 - Differences in dinucleotide frequencies of thermophilic genes encoding water soluble and membrane proteins
A1 - Hiroshi Nakashima
A1 - Yuka Kuroda
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 6
SP - 419
EP - 427
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000331

The occurrence frequencies of the dinucleotides of genes of three thermophilic and three mesophilic species from both archaea and eubacteria were investigated in this study. The genes encoding water soluble proteins were rich in the dinucleotides of purine dimers, whereas the genes encoding membrane proteins were rich in pyrimidine dimers. The dinucleotides of purine dimers are the counterparts of pyrimidine dimers in a double-stranded DNA. The purine/pyrimidine dimers were favored in the thermophiles but not in the mesophiles, based on comparisons of observed and expected frequencies. This finding is in agreement with our previous study which showed that purine/pyrimidine dimers are positive factors that increase the thermal stability of DNA. The dinucleotides AA, AG, and GA are components of the codons of charged residues of Glu, Asp, Lys, and Arg, and the dinucleotides TT, CT, and TC are components of the codons of hydrophobic residues of Leu, Ile, and Phe. This is consistent with the suitabilities of the different amino acid residues for water soluble and membrane proteins. Our analysis provides a picture of how thermophilic species produce water soluble and membrane proteins with distinctive characters: the genes encoding water soluble proteins use DNA sequences rich in purine dimers, and the genes encoding membrane proteins use DNA sequences rich in pyrimidine dimers on the opposite strand.

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