Full Text:   <1406>

Summary:  <1321>

CLC number: R375+.3

On-line Access: 2018-08-02

Received: 2018-01-04

Revision Accepted: 2018-02-09

Crosschecked: 2018-07-07

Cited: 0

Clicked: 3258

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Guang-yong Ye

https://orcid.org/0000-0001-5013-9797

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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.8 P.654-661

http://doi.org/10.1631/jzus.B1800005


Ureaplasma urealyticum-derived lipid-associated membrane proteins introduce IL-6, IL-8, and TNF-α cytokines into human amniotic epithelial cells via Toll-like receptor 2


Author(s):  Guang-yong Ye, Ke-yi Wang, Qiao-di Gui, Min Wang

Affiliation(s):  Women’s Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China; more

Corresponding email(s):   ygy20518158@zju.edu.cn

Key Words:  Ureaplasma urealyticum, Lipid-associated membrane protein, Human amniotic epithelial cell, Toll-like receptor 2


Guang-yong Ye, Ke-yi Wang, Qiao-di Gui, Min Wang. Ureaplasma urealyticum-derived lipid-associated membrane proteins introduce IL-6, IL-8, and TNF-α cytokines into human amniotic epithelial cells via Toll-like receptor 2[J]. Journal of Zhejiang University Science B, 2018, 19(8): 654-661.

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journal="Journal of Zhejiang University Science B",
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%T Ureaplasma urealyticum-derived lipid-associated membrane proteins introduce IL-6, IL-8, and TNF-α cytokines into human amniotic epithelial cells via Toll-like receptor 2
%A Guang-yong Ye
%A Ke-yi Wang
%A Qiao-di Gui
%A Min Wang
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T1 - Ureaplasma urealyticum-derived lipid-associated membrane proteins introduce IL-6, IL-8, and TNF-α cytokines into human amniotic epithelial cells via Toll-like receptor 2
A1 - Guang-yong Ye
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A1 - Qiao-di Gui
A1 - Min Wang
J0 - Journal of Zhejiang University Science B
VL - 19
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DOI - 10.1631/jzus.B1800005


Abstract: 
Objective: The purpose of this study was to determine the role of Ureaplasma urealyticum-derived lipid-associated membrane proteins (LAMPs) in the host innate immune system, specifically their effect on Toll-like receptors (TLRs). Methods: LAMPs were derived from U. urealyticum strains, and human amniotic epithelial cells (HAECs) were isolated from healthy full-term placentas. Cytokine concentrations were determined by enzyme-linked immunosorbent assay (ELISA) and TLR2 mRNA by real-time PCR. Expression of TLR2 was confirmed by Western blotting and immunohistochemistry. Results: LAMPs induced HAECs to produce inflammatory cytokines interleukin (IL)-6, IL-8, and tumor necrosis factor (TNF)-α. Cytokine production was reduced after blocking TLR2 using TLR2 inhibitor (anti-hTLR2-IgA). Conclusions: LAMPs isolated from U. urealyticum induced TLR2-dependent up-regulation of inflammatory genes and cytokines in HAECs.

解脲脲原体脂质相关膜蛋白经Toll样受体2信号通路调控人羊膜上皮细胞诱导IL-6、IL-8和TNF-α的产生

目的:探讨解脲脲原体(Ureaplasma urealyticum)及其脂质相关膜蛋白(LAMPs)作用于人羊膜上皮细胞(HAECs)过程中白介素6(IL-6)、IL-8和肿瘤坏死因子α(TNF-α)的变化情况,阐明Toll样受体2(TLR2)的调控机制,明确解脲脲原体潜在的致病性.
创新点:从解脲脲原体诱导炎症反应的分子机制入手,提出TLR2信号通路在其中的关键作用.
方法:经TX-114处理萃取解脲脲原体获得LAMPs,将LAMPs和解脲脲原体分别感染HAECs,用酶联免疫吸附试验(ELISA)测定炎症细胞因子 (IL-6、IL-8和TNF-α);采用实时聚合酶链反应(real-time PCR)测定TLR2 mRNA水平,用蛋白质印迹(Western blot)检测TLR2的表达量;经TLR2阻断剂(anti-hTLR2-IgA)处理后,测定相应炎症细胞因子.
结论:解脲脲原体LAMPs能诱导HAECs的TLR2表达上调和炎症因子增加,从而发生炎症反应;TLR2受阻断后,炎症因子表达减少,炎症水平下降.TLR2在解脲脲原体LAMPs感染HAECs过程起关键作用.

关键词:解脲脲原体;脂质相关膜蛋白;人羊膜上皮细胞;Toll样受体2

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