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Journal of Zhejiang University SCIENCE A 2004 Vol.5 No.4 P.462-466

http://doi.org/10.1631/jzus.2004.0462


Determination of the genus-specific antigens in outer membrane proteins from the strains of Leptospira interrogans and Leptospira biflexa with different virulence


Author(s):  LUO Yi-hui, YAN Jie, MAO Ya-fei, LI Shu-ping

Affiliation(s):  Department of Medical Microbiology and Parasitology, College of Medical Science, Zhejiang University, Hangzhou 310006, China

Corresponding email(s):   yanchen@mail.hz.zj.cn

Key Words:  Leptospira, Outer membrane protein, Genus-specific antigen


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LUO Yi-hui, YAN Jie, MAO Ya-fei, LI Shu-ping. Determination of the genus-specific antigens in outer membrane proteins from the strains of Leptospira interrogans and Leptospira biflexa with different virulence[J]. Journal of Zhejiang University Science A, 2004, 5(4): 462-466.

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author="LUO Yi-hui, YAN Jie, MAO Ya-fei, LI Shu-ping",
journal="Journal of Zhejiang University Science A",
volume="5",
number="4",
pages="462-466",
year="2004",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2004.0462"
}

%0 Journal Article
%T Determination of the genus-specific antigens in outer membrane proteins from the strains of Leptospira interrogans and Leptospira biflexa with different virulence
%A LUO Yi-hui
%A YAN Jie
%A MAO Ya-fei
%A LI Shu-ping
%J Journal of Zhejiang University SCIENCE A
%V 5
%N 4
%P 462-466
%@ 1869-1951
%D 2004
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2004.0462

TY - JOUR
T1 - Determination of the genus-specific antigens in outer membrane proteins from the strains of Leptospira interrogans and Leptospira biflexa with different virulence
A1 - LUO Yi-hui
A1 - YAN Jie
A1 - MAO Ya-fei
A1 - LI Shu-ping
J0 - Journal of Zhejiang University Science A
VL - 5
IS - 4
SP - 462
EP - 466
%@ 1869-1951
Y1 - 2004
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2004.0462


Abstract: 
Objective: To determine the existence of genus-specific antigens in outer membrane proteins (OMPs) of leptospira with different virulence. Methods: Microscope agglutination test (MAT) was applied to detect the agglutination between commercial rabbit antiserum against leptospiral genus-specific TR/Patoc I antigen and 17 strains of leptospira interrongans belonging to 15 serogroups and 2 strains of leptospira biflexa belonging to 2 serogroups. The outer envelopes (OEs) of L. interrogans serogroup Icterohaemorrhagiaeserovar lai strain lai (56601) with strong virulence and serogroup Pomona serovar pomonastrain Luo (56608) with low virulence, and L. biflexa serogroup Semaranga serovar patocstrain Patoc I without virulence were prepared by using the method reported in Auran et al.(1972). OMPs in the OEs were obtained by treatment with sodium deoxycholate. SDS-PAGE and western blot were used for analyzing the features of the OMPs on electrophoretic pattern and the immunoreactivity to the antiserum against TR/Patoc I antigen, respectively. Results: All the tested strains belonging to different leptospiral serogroups agglutinated to the antiserum against leptospiral genus-specific TR/Patoc I antigen with agglutination titers ranging from 1:256-1:512. A similar SDS-PAGE pattern of the OMPs from the three strains ofleptospira with different virulence was shown and the molecular weight of a major protein fragment in the OMPs was found to be approximately 60 KDa. A positive protein fragment with approximately 32 KDa confirmed by Western blot, was able to react with the antiserum against leptospiral genus-specific TR/Patoc I antigen, and was found in each the OMPs of the three stains of leptospira. Conclusion: There are genus-specific antigens on the surface of L. interrogans and L. biflexa. The OMP with molecular weight of 32 KDa may be one of the genus-specific protein antigens of leptospira.

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Anupama@Siriwardana<asiriwardana88@gmail.com>

2014-11-27 11:30:59

This articles is very important in finding L.biflexa antigenic profile.

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