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CLC number: R944.9

On-line Access: 2011-07-04

Received: 2010-01-07

Revision Accepted: 2011-04-16

Crosschecked: 2011-06-16

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Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.7 P.563-567

http://doi.org/10.1631/jzus.B1100006


Microcalorimetry studies on the antibacterial effect of crude monkshood polysaccharide


Author(s):  Gui-mei Lin, Lian Liu, Wei Shao

Affiliation(s):  School of Pharmaceutical Science, Shandong University, Jinan 250012, China

Corresponding email(s):   guimeilin@sdu.edu.cn, shaowei@sdu.edu.cn

Key Words:  Crude monkshood polysaccharide, Microcalorimetry, Escherichia coli, Staphylococcus aureus, Inhibitory


Gui-mei Lin, Lian Liu, Wei Shao. Microcalorimetry studies on the antibacterial effect of crude monkshood polysaccharide[J]. Journal of Zhejiang University Science B, 2011, 12(7): 563-567.

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author="Gui-mei Lin, Lian Liu, Wei Shao",
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doi="10.1631/jzus.B1100006"
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%T Microcalorimetry studies on the antibacterial effect of crude monkshood polysaccharide
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%A Lian Liu
%A Wei Shao
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 7
%P 563-567
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1100006

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T1 - Microcalorimetry studies on the antibacterial effect of crude monkshood polysaccharide
A1 - Gui-mei Lin
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A1 - Wei Shao
J0 - Journal of Zhejiang University Science B
VL - 12
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EP - 567
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1100006


Abstract: 
In this paper, crude monkshood polysaccharide was isolated from Radix Aconiti Lateralis Preparata. The effects of crude monkshood polysaccharide on Escherichia coli and Staphylococcus aureus were investigated by microcalorimetry. The power-time curves of the bacterial growth at various concentrations (c) of crude monkshood polysaccharide were plotted with a TAM air isothermal microcalorimeter at 37 °C. The growth rate constant (μ), inhibitory ratio (I), peak-height (Pm), and peak-time (tm) were calculated. From the data, the relationship between μ and c also was established. The growth rate constant μ decreased with the increasing concentrations of crude monkshood polysaccharide. Moreover, Pm reduced and tm increased with increasing concentrations. The experimental results revealed that crude monkshood polysaccharide had inhibitory activity towards S. aureus and E. coli. Results obtained from our study strongly suggest that microcalorimetry is a fast, simple, and more sensitive technology that can be easily performed to study the effect of drugs on bacteria.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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