Full Text:   <1863>

Summary:  <1612>

CLC number: R285.1

On-line Access: 2015-08-04

Received: 2015-05-18

Revision Accepted: 2015-07-22

Crosschecked: 2015-07-24

Cited: 3

Clicked: 3051

Citations:  Bibtex RefMan EndNote GB/T7714


Gui-mei Lin


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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.8 P.690-695


Microcalorimetry studies of the antimicrobial actions of Aconitum alkaloids

Author(s):  Yan-bin Shi, Lian Liu, Wei Shao, Ting Wei, Gui-mei Lin

Affiliation(s):  1School of Mechanical and Automotive Engineering, Qilu University of Technology, Jinan 250353, China; more

Corresponding email(s):   guimeilin@sdu.edu.cn

Key Words:  Aconitum alkaloids, Escherichia coli, Staphylococcus aureus, Microcalorimetry

Yan-bin Shi, Lian Liu, Wei Shao, Ting Wei, Gui-mei Lin. Microcalorimetry studies of the antimicrobial actions of Aconitum alkaloids[J]. Journal of Zhejiang University Science B, 2015, 16(8): 690-695.

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DOI - 10.1631/jzus.B1500121

The metabolic activity of organisms can be measured by recording the heat output using microcalorimetry. In this paper, the total alkaloids in the traditional Chinese medicine Radix Aconiti Lateralis were extracted and applied to Escherichia coli and Staphylococcus aureus. The effect of alkaloids on bacteria growth was studied by microcalorimetry. The power-time curves were plotted with a thermal activity monitor (TAM) air isothermal microcalorimeter and parameters such as growth rate constant (μ), peak-time (Tm), inhibitory ratio (I), and enhancement ratio (E) were calculated. The relationships between the concentration of Aconitum alkaloids and μ of E. coli or S. aureus were discussed. The results showed that Aconitum alkaloids had little effect on E. coli and had a potentially inhibitory effect on the growth of S. aureus.


方法:微量量热仪绘制了37 °C 时大肠杆菌和金黄色葡萄球菌在不同浓度的附子生物碱作用下的热谱曲线,根据热动力学模型,分别得到了生长速率常数µ、抑制率I、最大产热功率Pm以及最大产热功率所对应的时间Tm等热动力学参数,从热动力学的角度探讨了附子生物碱对大肠杆菌和金黄色葡萄球菌代谢作用的影响。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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