Full Text:   <2349>

CLC number: R764.35

On-line Access: 2011-10-08

Received: 2011-02-10

Revision Accepted: 2011-07-27

Crosschecked: 2011-08-31

Cited: 1

Clicked: 4667

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.10 P.853-861

http://doi.org/10.1631/jzus.B1100040


Ototoxic destruction by co-administration of kanamycin and ethacrynic acid in rats


Author(s):  Hong Liu, Da-lian Ding, Hai-yan Jiang, Xue-wen Wu, Richard Salvi, Hong Sun

Affiliation(s):  Center for Hearing and Deafness, University at Buffalo, State University of New York, New York 14214, USA, Department of Otolaryngology, the Third Xiangya Hospital of Central South University, Changsha 410013, China, Department of Otolaryngology, Xiangya Hospital of Central South University, Changsha 410008, China

Corresponding email(s):   dding@buffalo.edu

Key Words:  Ototoxicity, Ethacrynic acid, Kanamycin, Rat, Blood-cochlea barrier


Hong Liu, Da-lian Ding, Hai-yan Jiang, Xue-wen Wu, Richard Salvi, Hong Sun. Ototoxic destruction by co-administration of kanamycin and ethacrynic acid in rats[J]. Journal of Zhejiang University Science B, 2011, 12(10): 853-861.

@article{title="Ototoxic destruction by co-administration of kanamycin and ethacrynic acid in rats",
author="Hong Liu, Da-lian Ding, Hai-yan Jiang, Xue-wen Wu, Richard Salvi, Hong Sun",
journal="Journal of Zhejiang University Science B",
volume="12",
number="10",
pages="853-861",
year="2011",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1100040"
}

%0 Journal Article
%T Ototoxic destruction by co-administration of kanamycin and ethacrynic acid in rats
%A Hong Liu
%A Da-lian Ding
%A Hai-yan Jiang
%A Xue-wen Wu
%A Richard Salvi
%A Hong Sun
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 10
%P 853-861
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1100040

TY - JOUR
T1 - Ototoxic destruction by co-administration of kanamycin and ethacrynic acid in rats
A1 - Hong Liu
A1 - Da-lian Ding
A1 - Hai-yan Jiang
A1 - Xue-wen Wu
A1 - Richard Salvi
A1 - Hong Sun
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 10
SP - 853
EP - 861
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1100040


Abstract: 
It is well known that ethacrynic acid (EA) can potentiate the ototoxicity of aminoglycoside antibiotics (AmAn) such as kanamycin (KM), if they were applied at the same time. Currently, to create the model of EA-KM-induced cochlear lesion in rats, adult rats received a single injection of EA (75 mg/kg, intravenous injection), or followed immediately by KM (500 mg/kg, intramuscular injection). The hearing function was assessed by auditory brainstem response (ABR) measurement in response to click and/or tone bursts at 4, 8, 12, 16, 20, 24, and 32 kHz. The static microcirculation status in the stria vascularis after a single EA injection was evaluated with eosin staining. The pathological changes in cochlear and vestibular hair cells were also quantified after co-administration of EA and KM. After a single EA injection, blood flow in vessels supplying the stria vascularis rapidly diminished. However, the blood supply to the cochlear lateral wall partially recovered 5 h after EA treatment. Threshold changes in ABR were basically parallel to the microcirculation changes in stria vascularis after single EA treatment. Importantly, disposable co-administration of EA and KM resulted in a permanent hearing loss and severe damage to the cochlear hair cells, but spared the vestibular hair cells. Since the cochlear lateral wall is the important part of the blood-cochlea barrier, EA-induced anoxic damage to the epithelium of stria vascularis may enhance the entry of KM to the cochlea. Thus, experimental animal model of selective cochlear damage with normal vestibular systems can be reliably created through co-administration of EA and KM.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]Abbruzzese, J.L., Amato, R., Schmidt, S., Raber, M.N., Frost, P., 1990. Phase I clinical trial of cisplatin given i.v. with 5-fluorouracil and high-dose folinic acid. Cancer Chemother. Pharmacol., 26(3):159-162.

[2]Ajodhia, J.M., Dix, M.R., 1976. Drug-induced deafness and its treatment. Practitioner, 216(1295):561-570.

[3]Akisada, T., 1987. Evaluation of cochlear damage in kanamycin administered hamster and protective effect of cepharanthine against KM induced ototoxicity. An experimental study. Nippon Jibiinkoka Gakkai Kaiho, 90(8):1229-1244.

[4]Akiyoshi, M., 1978. Evaluation of ototoxicity of tobramycin in guinea pigs. J. Antimicrob. Chemother., 4(Suppl. A):69-72.

[5]Akiyoshi, M., Sato, K., Nakada, H., Tajima, T., 1974. Audiometric and histopathologic evaluation of ototoxicity of 3′,4′-dideoxykanamycin B, a new aminoglycoside antibiotic (author’s transl.). Jpn. J. Antibiot., 27(1):15-26 (in Japanese).

[6]Akiyoshi, M., Sato, K., Nakada, H., Tajima, T., Suzuki, K., 1975. Evaluation of ototoxicity of amikacin (BB-K8) by animal test (author’s transl.). Jpn. J. Antibiot., 28(3):288-304 (in Japanese).

[7]Aran, J.M., 1982. Evaluation of the ototoxicity of aminoglycosides. Comparative study of dibekacin, gentamicin and tobramycin. Nouv. Presse. Med., 11(46):3426-3431.

[8]Aran, J.M., Erre, J.P., Lima da Costa, D., Debbarh, I., Dulon, D., 1999. Acute and chronic effects of aminoglycosides on cochlear hair cells. Ann. N. Y. Acad. Sci., 884:60-68.

[9]Au, S., Weiner, N., Schacht, J., 1986. Membrane perturbation by aminoglycosides as a simple screen of their toxicity. Antimicrob. Agents Chemother., 30(3):395-397.

[10]Bailey, R.R., Peddie, B., 1976. Tobramycin in the treatment of severe and complicated urinary tract infections. N. Z. Med. J., 84(567):1-3.

[11]Becvarovski, Z., Bojrab, D.I., Michaelides, E.M., Kartush, J.K., Zappia, J.J., LaRouere, M.J., 2002. Round window gentamicin absorption: an in vivo human model. Laryngoscope, 112(9):1610-1613.

[12]Chen, G.D., Kermany, M.H., D′Elia, A., Ralli, M., Tanaka, C., Bielefeld, E.C., Ding, D., Henderson, D., Salvi, R., 2010. Too much of a good thing: long-term treatment with salicylate strengthens outer hair cell function but impairs auditory neural activity. Hear. Res., 265(1-2):63-69.

[13]de Jager, P., van Altena, R., 2002. Hearing loss and nephrotoxicity in long-term aminoglycoside treatment in patients with tuberculosis. Int. J. Tuberc. Lung Dis., 6(7):622-627.

[14]Ding, D., Zhang, Z., 1995. Acoustical transmission blockage caused by urethane and streptomycin. J. Audiol. Speech Disord., 3(1):36-38 (in Chinese).

[15]Ding, D., Salvi, R., 2005. Review of cellular changes in the cochlea due to aminoglycoside antibiotics. Volta Rev., 105(3):407-438.

[16]Ding, D., Luo, D., Huangfu, M., 1990a. The kanamycin toxic relation between the ear and kidney. J. Clin. Otorhinolaryngol., 4(3):142-144 (in Chinese).

[17]Ding, D., Zhao, J., Luo, D., Huangfu, M., 1990b. The microcirculation static quantitative observation of the stria vascularis. Acta Otolaryngol., 4(2):1-2 (in Chinese).

[18]Ding, D., Luo, D., Guo. Y., Huangfu, M., 1991. Probe into the ototoxic mechanism of aminoglycoside antibiotic. Chin. J. Otorhinolaryngol., 26(3):154-155 (in Chinese).

[19]Ding, D., Chen, X., Jin, X., 1992. Observation of vestibular end organ with a small field vision count technique. Chin. J. Otorhinolaryngol., 27(4):202-203 (in Chinese).

[20]Ding, D., Jin, X., Huangfu, M., 1993. Acoustical transmission blockage caused by streptomycin. J. Audiol. Speech Disord., 1(1):29-31 (in Chinese).

[21]Ding, D., Jin, X., Zhao, J., 1995a. Accumulative sites of kanamycin in cochlea basal membrane cells. Chin. J. Otorhinolaryngol., 30(6):323-325 (in Chinese).

[22]Ding, D., Jin, X., Zhao, J., 1995b. Different binding sites of kanamycin and streptomycin in the organs of Corti. J. Clin. Otorhinolaryngol., 9(6):346-347 (in Chinese).

[23]Ding, D., Jin, X., Zhang, Z., Zhu, Q., 1995c. Different susceptibility in gentamycin ototoxicity between red and black eye guinea pigs. Acta Otorhinolaryngol., 9(2):70-74 (in Chinese).

[24]Ding, D., Zhang, Z., Zhu, Q., 1995d. Experimental study of concurrent ototoxicity between ethacrynic acid and gentamycin. J. Audiol. Speech Disord., 3(2):76-79 (in Chinese).

[25]Ding, D., Jin, X., Zhao, J., 1996. The changes of cochlear bioelectric potential on guinea pigs deafened with ethacrynic acid. J. Clin. Otorhinolaryngol., 10(6):330-332 (in Chinese).

[26]Ding, D., Jin, X., Zhao, J., 1997. Accumulative sites of kanamycin in the organ of Corti by microautoradiography. Chin. J. Otorhinolaryngol., 32(6):348-349 (in Chinese).

[27]Ding, D., McFadden, S.L., Woo, J.M., Salvi, R., 2002. Ethacrynic acid rapidly and selectively abolishes blood flow in vessels supplying the lateral wall of the cochlea. Hear. Res., 173(1-2):1-9.

[28]Ding, D., McFadden, S.L., Browne, R.W., Salvi, R., 2003. Late dosing with ethacrynic acid can reduce gentamicin concentration in perilymph and protect cochlear hair cells. Hear. Res., 185(1-2):90-96.

[29]Ding, D., Jiang, H., McFadden, S.L., Salvi, R., 2004. Ethacrynic acid is the key for opening of the blood-labyrinth barrier. Chin. J. Otol., 1(2):42-47 (in Chinese).

[30]Ding, D., Jiang, H., Wang, P., Salvi, R., 2007. Cell death after co-administration of cisplatin and ethacrynic acid. Hear. Res., 226(1-2):129-139.

[31]Ding, D., Qi, W., Zhang, M., Wang, P., Jiang, H., Salvi, R., 2008. Cisplatin and its ototoxicity. Chin. J. Otol., 6(2):125-133 (in Chinese).

[32]Ding, D., Jiang, H., Salvi, R., 2010. Mechanisms of rapid sensory hair-cell death following co-administration of gentamicin and ethacrynic acid. Hear. Res., 259(1-2):16-23.

[33]Graham, A.C., Mercier, R.C., Achusim, L.E., Pai, M.P., 2004. Extended-interval aminoglycoside dosing for treatment of enterococcal and staphylococcal osteomyelitis. Ann. Pharmacother., 38(6):936-941.

[34]Greenwood, D.D., 1990. A cochlear frequency-position function for several species-29 years later. J. Acoust. Soc. Am., 87(6):2592-2605.

[35]Guan, M.X., Fischel-Ghodsian, N., Attardi, G., 2000. A biochemical basis for the inherited susceptibility to aminoglycoside ototoxicity. Hum. Mol. Genet., 9(12):1787-1793.

[36]Gutell, R.R., Larsen, N., Woese, C.R., 1994. Lessons from an evolving rRNA: 16S and 23S rRNA structures from a comparative perspective. Microbiol. Rev., 58(1):10-26.

[37]Hangfu, M., Zhao, J., Ding, D., 1992. The prophylactic effect of thyroxin on kanamycin ototoxicity in guinea pigs. Hear. Res., 61(1-2):132-136.

[38]Humes, H.D., 1999. Insights into ototoxicity. Analogies to nephrotoxicity. Ann. N. Y. Acad. Sci., 884(1):15-18.

[39]Kraus, K.S., Ding, D., Zhou, Y., Salvi, R., 2009. Central auditory plasticity after carboplatin-induced unilateral inner ear damage in the chinchilla: up-regulation of GAP-43 in the ventral cochlear nucleus. Hear. Res., 255(1-2):33-43.

[40]Li, M., Ding, D., Zheng, X.Y., Salvi, R., 2004. Vestibular destruction by slow infusion of gentamicin into semicircular canals. Acta Otolaryngol., 124(Suppl. 552):35-41.

[41]Li, Y., Ding, D., Jiang, H., Fu, Y., Salvi, R., 2011. Co-administration of cisplatin and furosemide causes rapid and massive loss of cochlear hair cells in mice. Neurot. Res., in press.

[42]McFadden, S.L., Ding, D., Jiang, H., Woo, J.M., Salvi, R., 2002. Chinchilla models of selective cochlear hair cell loss. Hear. Res., 174(1-2):230-238.

[43]Moazed, D., Noller, H.F., 1987. Interaction of antibiotics with functional sites in 16S ribosomal RNA. Nature, 327(6121):389-394.

[44]Pittinger, C., Adamson, R., 1972. Antibiotic blockade of neuromuscular function. Annu. Rev. Pharmacol., 12:169-184.

[45]Recht, M.I., Douthwaite, S., Dahlquist, K.D., Puglisi, J.D., 1999. Effect of mutations in the A site of 16S rRNA on aminoglycoside antibiotic-ribosome interaction. J. Mol. Biol., 286(1):33-43.

[46]Rougier, F., Claude, D., Maurin, D., Sedoglavic, A., Ducher, M., Corvaisier, S., Jelliffe, R., Maire, P., 2003. Aminoglycoside nephrotoxicity: modeling, simulation, and control. Antimicrob. Agents Chemother., 47(3):1010-1016.

[47]Rougier, F., Claude, D., Maurin, M., Maire, P., 2004. Aminoglycoside nephrotoxicity. Curr. Drug Targets Infect. Disord., 4(2):153-162.

[48]Shuman, R.D., Smith, C.R., 1978. Intrathecal gentamicin for refractory gram-positive meningitis. JAMA, 240(5):469-471.

[49]Swan, E.E., Mescher, M.J., Sewell, W.F., Tao, S.L., Borenstein, J.T., 2008. Inner ear drug delivery for auditory applications. Adv. Drug Deliv. Rev., 60(15):1583-1599.

[50]Torihara, K., Suganuma, T., Ide, S., Morimitsu, T., 1994. Anionic sites in blood capillaries of the mouse cochlear duct. Hear. Res., 77(1-2):69-74.

[51]Vellai, T., Takacs, K., Vida, G., 1998. A new aspect to the origin and evolution of eukaryotes. J. Mol. Evol., 46(5):499-507.

[52]Versnel, H., Agterberg, M.J., de Groot, J.C., Smoorenburg, G.F., Klis, S.F., 2007. Time course of cochlear electrophysiology and morphology after combined administration of kanamycin and furosemide. Hear. Res., 231(1-2):1-12.

[53]Wilhelm, J.M., Pettitt, S.E., Pettitt, S.E., 1978. Aminoglycoside antibiotics and eukaryotic protein synthesis: structure-function relationships in the stimulation of misreading with a wheat embryo system. Biochemistry, 17(7):1143-1149.

[54]Wu, W.J., Sha, S.H., McLaren, J.D., Kawamoto, K., Raphael, Y., Schacht, J., 2001. Aminoglycoside ototoxicity in adult CBA, C57BL and BALB mice and the Sprague-Dawley rat. Hear. Res., 158(1-2):165-178.

[55]Xu, S.A., Shepherd, R.K., Chen, Y., Clark, G.M., 1993. Profound hearing loss in the cat following the single co-administration of kanamycin and ethacrynic acid. Hear. Res., 70(2):205-215.

[56]Yamasoba, T., Kondo, K., Miyajima, C., Suzuki, M., 2003. Changes in cell proliferation in rat and guinea pig cochlea after aminoglycoside-induced damage. Neurosci. Lett., 347(3):171-174.

[57]Zhao, J., Ding, D., Huangfu, M., 1988a. The influence of enthacrynic acid on the activity of enzyme in the stria vascularis in guinea pigs. J. Clin. Otorhinolaryngol., 2(3):65-67 (in Chinese).

[58]Zhao, J., Ding, D., Wang, J., Huangfu, M., 1988b. Influence of ethacrynic acid on microcirculation of stria vascularis of cochlea in guinea pigs. Acta Univ. Med. Second. Shanghai, 8(2):34-37 (in Chinese).

[59]Zhou, Y., Ding, D., Kraus, K.S., Yu, D., Salvi, R., 2009. Functional and structural changes in the chinchilla cochlea and vestibular system following round window application of carboplatin. Audiol. Med. 7(4):189-199.

[60]Zhu, Q., Liu, G., Ding, D., Jin, X., 1993. Accumulation of gentamycin in perilymph of guinea pigs. Acad. J. Second Mil. Med. Univ., 14(6):568-571 (in Chinese).

[61]Zucca, G., Vega, R., Botta, L., Perez, M.E., Valli, P., Soto, E., 1992. Streptomycin blocks the afferent synapse of the isolated semicircular canals of the frog. Hear. Res., 59(1):70-74.

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2022 Journal of Zhejiang University-SCIENCE