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CLC number: R574.62

On-line Access: 2015-03-05

Received: 2014-07-07

Revision Accepted: 2014-12-03

Crosschecked: 2015-02-17

Cited: 6

Clicked: 3227

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Joshua J. Malago

http://orcid.org/0000-0002-8903-210X

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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.3 P.224-234

http://doi.org/10.1631/jzus.B1400191


Intraperitoneal administration of butyrate prevents the severity of acetic acid colitis in rats


Author(s):  Joshua J. Malago, Catherine L. Sangu

Affiliation(s):  Department of Pathology, Faculty of Veterinary Medicine, Sokoine University of Agriculture, P.O. Box 3203, Morogoro, Tanzania; more

Corresponding email(s):   malagojj@yahoo.com

Key Words:  Butyrate, Oral administration, Intraperitoneal administration, Intrarectal administration, Acetic acid, Ulcerative colitis


Joshua J. Malago, Catherine L. Sangu. Intraperitoneal administration of butyrate prevents the severity of acetic acid colitis in rats[J]. Journal of Zhejiang University Science B, 2015, 16(3): 224-234.

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author="Joshua J. Malago, Catherine L. Sangu",
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year="2015",
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doi="10.1631/jzus.B1400191"
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%T Intraperitoneal administration of butyrate prevents the severity of acetic acid colitis in rats
%A Joshua J. Malago
%A Catherine L. Sangu
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T1 - Intraperitoneal administration of butyrate prevents the severity of acetic acid colitis in rats
A1 - Joshua J. Malago
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1400191


Abstract: 
Intrarectal infusion of butyrate improves colorectal disorders including ulcerative colitis (UC). However, it is not established whether systemically administered butyrate benefits such patients. The current study aimed at exploring and comparing the potential of intraperitoneally, intrarectally, and orally administered butyrate against acetic acid (AA)-induced UC in rats. intrarectal administration of 2 ml of 50% AA was done after or without prior treatment of rats for 7 consecutive days with 100 mg/kg sodium butyrate (SB) intraperitoneally, intrarectally, or orally. Rats were sacrificed after 48 h of AA-treatment. Subsequently, colon sections were processed routinely for histopathological examination. We clinically observed diarrhea, loose stools, and hemoccult-positive stools, and histologically, epithelial loss and ulceration, crypt damage, goblet cell depletion, hemorrhage, and mucosal infiltration of inflammatory cells. The changes were significantly reduced by intraperitoneal, intrarectal, or oral butyrate, with intraperitoneal butyrate exhibiting the highest potency. It is concluded that intraperitoneal administration of butyrate abrogates the lesions of AA-induced UC and its potency surpasses that of intrarectal or oral butyrate.

腹腔注射丁酸盐对大鼠乙酸性结肠炎的预防作用

中文概要:
目的:探索腹腔注射丁酸盐对防止乙酸性结肠炎的疗效。
创新点:首次对大鼠进行腹腔注射丁酸盐,通过与直肠灌注和口服比较,探索三种不同给药方式对预防乙酸性结肠炎的疗效差异。
方法:以40只Wistar大鼠为实验对象,分组进行连续7天的腹腔注射、直肠灌注和口服100mg/kg丁酸钠(SB),第8天进行乙酸(AA)直肠灌注,48小时后处死。记录实验大鼠的临床症状,包括体重减少、腹泻、便血等。对结肠切片进行组织病理学观察,最后对试验数据进行统计分析。
结论:腹腔注射、直肠灌注和口服丁酸盐均能明显缓解大鼠乙酸性结肠炎的炎症,其中以腹腔注射疗效最佳。

关键词:丁酸盐;口服;腹腔注射;直肠灌注;乙酸;溃疡性结肠炎

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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