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CLC number: R73

On-line Access: 2011-10-08

Received: 2011-03-01

Revision Accepted: 2011-06-02

Crosschecked: 2011-09-13

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Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.10 P.812-819

http://doi.org/10.1631/jzus.B1100059


Genome-wide analysis of OCT4 binding sites in glioblastoma cancer cells


Author(s):  Xue-feng Fang, Wei-yi Zhang, Na Zhao, Wei Yu, Dong Ding, Xu Hong, Li-sha Li, Hua-rong Zhang, Shu Zheng, Biao-yang Lin

Affiliation(s):  Key Laboratory of Cancer Prevention and Intervention of Ministry of Education, Key Laboratory of Molecular Biology in Medical Sciences of Zhejiang Province, Cancer Institute, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China, Systems Biology Division, Zhejiang-California International Nanosystems Institute (ZCNI), Zhejiang University, Hangzhou 310029, China

Corresponding email(s):   zhengshu@zju.edu.cn, biaoylin@zju.edu.cn

Key Words:  OCT4, Chromatin immunoprecipitation-sequencing (ChIP-seq), DNA binding region, GlioblastomaThe online version of this article contains supplementary materials


Xue-feng Fang, Wei-yi Zhang, Na Zhao, Wei Yu, Dong Ding, Xu Hong, Li-sha Li, Hua-rong Zhang, Shu Zheng, Biao-yang Lin. Genome-wide analysis of OCT4 binding sites in glioblastoma cancer cells[J]. Journal of Zhejiang University Science B, 2011, 12(10): 812-819.

@article{title="Genome-wide analysis of OCT4 binding sites in glioblastoma cancer cells",
author="Xue-feng Fang, Wei-yi Zhang, Na Zhao, Wei Yu, Dong Ding, Xu Hong, Li-sha Li, Hua-rong Zhang, Shu Zheng, Biao-yang Lin",
journal="Journal of Zhejiang University Science B",
volume="12",
number="10",
pages="812-819",
year="2011",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1100059"
}

%0 Journal Article
%T Genome-wide analysis of OCT4 binding sites in glioblastoma cancer cells
%A Xue-feng Fang
%A Wei-yi Zhang
%A Na Zhao
%A Wei Yu
%A Dong Ding
%A Xu Hong
%A Li-sha Li
%A Hua-rong Zhang
%A Shu Zheng
%A Biao-yang Lin
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 10
%P 812-819
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1100059

TY - JOUR
T1 - Genome-wide analysis of OCT4 binding sites in glioblastoma cancer cells
A1 - Xue-feng Fang
A1 - Wei-yi Zhang
A1 - Na Zhao
A1 - Wei Yu
A1 - Dong Ding
A1 - Xu Hong
A1 - Li-sha Li
A1 - Hua-rong Zhang
A1 - Shu Zheng
A1 - Biao-yang Lin
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 10
SP - 812
EP - 819
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1100059


Abstract: 
OCT4, a member of the POU family of gene products, is an octamer motif-binding transcription factor. As it is known to play a crucial role in cancer processes including proliferation, invasion, and chemoradioresistance, it is important to identify the direct targets of OCT4 in living cancer cells. Here, chromatin immunoprecipitation-sequencing (ChIP-seq) was used to identify OCT4 binding sites in glioblastoma cancer cells. The results showed that 5438 OCT4 binding sites were localized in the glioblastoma cancer genome and that these sites contained a consensus sequence TTTkswTw (k=T or G, s=C or G, w=A or T), which occurred 3931 times in 2312 OCT4 binding regions. Furthermore, binding motifs of some other transcription factors were identified in OCT4 binding regions. Our results provide a valuable dataset for understanding gene regulation mechanisms underlying the function of OCT4 in glioblastoma cancer.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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