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Journal of Zhejiang University SCIENCE B 2012 Vol.13 No.1 P.1-10


Different sperm sources and parameters can influence intracytoplasmic sperm injection outcomes before embryo implantation

Author(s):  Yue-hong Lu, Hui-juan Gao, Bai-jia Li, Ying-ming Zheng, Ying-hui Ye, Yu-li Qian, Chen-ming Xu, He-feng Huang, Fan Jin

Affiliation(s):  Department of Reproductive Endocrinology, Key Laboratory of Reproductive Genetics, Ministry of Education, and Key Laboratory of Womens Reproductive Health of Zhejiang Province, Womens Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China, Department of Reproductive Health, Shaoxing Women and Children Hospital, Shaoxing 312000, China

Corresponding email(s):   jinfan@zju.edu.cn

Key Words:  Intracytoplasmic sperm injection (ICSI), Sperm, Sperm source, Sperm parameters, Malformation

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Yue-hong Lu, Hui-juan Gao, Bai-jia Li, Ying-ming Zheng, Ying-hui Ye, Yu-li Qian, Chen-ming Xu, He-feng Huang, Fan Jin. Different sperm sources and parameters can influence intracytoplasmic sperm injection outcomes before embryo implantation[J]. Journal of Zhejiang University Science B, 2012, 13(1): 1-10.

@article{title="Different sperm sources and parameters can influence intracytoplasmic sperm injection outcomes before embryo implantation",
author="Yue-hong Lu, Hui-juan Gao, Bai-jia Li, Ying-ming Zheng, Ying-hui Ye, Yu-li Qian, Chen-ming Xu, He-feng Huang, Fan Jin",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Different sperm sources and parameters can influence intracytoplasmic sperm injection outcomes before embryo implantation
%A Yue-hong Lu
%A Hui-juan Gao
%A Bai-jia Li
%A Ying-ming Zheng
%A Ying-hui Ye
%A Yu-li Qian
%A Chen-ming Xu
%A He-feng Huang
%A Fan Jin
%J Journal of Zhejiang University SCIENCE B
%V 13
%N 1
%P 1-10
%@ 1673-1581
%D 2012
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1100216

T1 - Different sperm sources and parameters can influence intracytoplasmic sperm injection outcomes before embryo implantation
A1 - Yue-hong Lu
A1 - Hui-juan Gao
A1 - Bai-jia Li
A1 - Ying-ming Zheng
A1 - Ying-hui Ye
A1 - Yu-li Qian
A1 - Chen-ming Xu
A1 - He-feng Huang
A1 - Fan Jin
J0 - Journal of Zhejiang University Science B
VL - 13
IS - 1
SP - 1
EP - 10
%@ 1673-1581
Y1 - 2012
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1100216

To evaluate the effects of sperm with different parameters and sources on the outcomes of sperm injection (ICSI)%29&ck%5B%5D=abstract&ck%5B%5D=keyword'>intracytoplasmic sperm injection (ICSI), 1972 ICSI cycles were analyzed retrospectively. Groups 1 to 5 were composed of cycles using ejaculated sperm and were grouped according to sperm quantity, quality, and morphology into normal (288 cycles), or mild (329 cycles), moderate (522 cycles), severe (332 cycles), and extremely severe (171 cycles) oligozoospermia and/or asthenozoospermia and/or teratozoospermia (OAT) groups. Group 6 was composed of 250 cycles using testicular or epididymal sperm, and Group 7 consisted of 80 cycles using frozen-thawed sperm. We found that fertilization rates were gradually reduced from Groups 1 to 6, and reached statistical difference in Groups 5 and 6 (P<0.05). The high-quality embryo rate was higher in Group 1 than in Groups 2, 3, 5, 6, and 7 (P<0.05). No statistical differences were observed in the rates of embryo cleavage, clinical pregnancy, miscarriage, live-birth, premature birth, low birth weight, weeks of premature birth, average birth weight, or sex ratio for all seven groups (P>0.05). A total of nine cases of malformation were observed, with a malformation rate of 1.25% (9/719). In conclusion, different sperm sources and parameters can affect ICSI outcomes before embryo implantation. A full assessment of offspring malformation will require further study using a larger sample size.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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