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CLC number: TB13; R318.52

On-line Access: 2012-02-29

Received: 2011-02-20

Revision Accepted: 2011-07-26

Crosschecked: 2012-02-11

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Journal of Zhejiang University SCIENCE B 2012 Vol.13 No.3 P.213-220


Permeation of dimethyl sulfoxide into articular cartilage at subzero temperatures

Author(s):  Shao-zhi Zhang, Xiao-yi Yu, Guang-ming Chen

Affiliation(s):  Institute of Refrigeration and Cryogenics, Zhejiang University, Hangzhou 310027, China

Corresponding email(s):   enezsz@zju.edu.cn, gmchen@zju.edu.cn

Key Words:  Articular cartilage, Vitrification, Dimethyl sulfoxide, Permeation, Diffusion coefficient, Subzero temperature

Shao-zhi Zhang, Xiao-yi Yu, Guang-ming Chen. Permeation of dimethyl sulfoxide into articular cartilage at subzero temperatures[J]. Journal of Zhejiang University Science B, 2012, 13(3): 213-220.

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author="Shao-zhi Zhang, Xiao-yi Yu, Guang-ming Chen",
journal="Journal of Zhejiang University Science B",
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%0 Journal Article
%T Permeation of dimethyl sulfoxide into articular cartilage at subzero temperatures
%A Shao-zhi Zhang
%A Xiao-yi Yu
%A Guang-ming Chen
%J Journal of Zhejiang University SCIENCE B
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%P 213-220
%@ 1673-1581
%D 2012
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B11a0041

T1 - Permeation of dimethyl sulfoxide into articular cartilage at subzero temperatures
A1 - Shao-zhi Zhang
A1 - Xiao-yi Yu
A1 - Guang-ming Chen
J0 - Journal of Zhejiang University Science B
VL - 13
IS - 3
SP - 213
EP - 220
%@ 1673-1581
Y1 - 2012
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B11a0041

Osteochondral allografting has been proved to be a useful method to treat diseased or damaged areas of joint surfaces. Operational long-term stocks of grafts which supply a buffer between procurement and utilization would contribute to the commercialization or industrialization of this technology. vitrification has been thought to be a promising method for successful preservation of articular cartilage (AC), but high concentration cryoprotectants (CPAs) are used which may cause high cellular toxicity. An effective way to reduce CPA toxicity is to increase CPA concentration gradually while the temperature is lowered. Understanding the mechanism of CPA permeation at subzero temperatures is important for designing the cryopreservation protocol. In this research, the permeation of dimethyl sulfoxide (Me2SO) in ovine AC at subzero temperatures was studied experimentally. Pretreated AC discs were exposed in Me2SO solutions for different time (0, 5, 15, 30, 50, 80, and 120 min) at three temperature levels (−10, −20, and −30 °C). The Me2SO concentration within the tissue was determined by ultraviolet (UV) spectrophotometry. The diffusion coefficients were estimated to be 0.85×10−6, 0.48×10−6, and 0.27×10−6 cm2/s at −10, −20, and −30 °C, respectively, and the corresponding activation energy was 29.23 kJ/mol. Numerical simulation was performed to compare two Me2SO addition protocols, and the results demonstrated that the total loading duration could be effectively reduced with the knowledge of permeation kinetics.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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