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Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.3 P.200-208

http://doi.org/10.1631/jzus.B0900074


Effects of moniliformin and selenium on human articular cartilage metabolism and their potential relationships to the pathogenesis of Kashin-Beck disease


Author(s):  An Zhang, Jun-ling Cao, Bo Yang, Jing-hong Chen, Zeng-tie Zhang, Si-yuan Li, Qiang Fu, Clare E. Hugnes, Bruce Caterson

Affiliation(s):  Institute of Endemic Diseases, School of Medicine, Xi’an Jiaotong University, Xi’an 710061, China, Key Laboratory of Environment and Genes Related to Diseases of Ministry of Education, Xi’an Jiaotong University, Xi’an 710061, China, Key Laboratory of Microelement and Endemic Disease of Ministry of Health, Xi’an Jiaotong University, Xi’an 710061, China, 4һLaboratory of Connective Tissue Biology, School of Biosciences, Cardiff University, Cardiff CF10 3US, UK

Corresponding email(s):   caojl@mail.xjtu.edu.cn, caterson@cardiff.ac.uk

Key Words:  Chondrocytes, Moniliformin, Selenium, Aggrecan, Collagen, Matrix metalloproteinases (MMPs), CD44, Kashin-Beck disease


An Zhang, Jun-ling Cao, Bo Yang, Jing-hong Chen, Zeng-tie Zhang, Si-yuan Li, Qiang Fu, Clare E. Hugnes, Bruce Caterson. Effects of moniliformin and selenium on human articular cartilage metabolism and their potential relationships to the pathogenesis of Kashin-Beck disease[J]. Journal of Zhejiang University Science B, 2010, 11(3): 200-208.

@article{title="Effects of moniliformin and selenium on human articular cartilage metabolism and their potential relationships to the pathogenesis of Kashin-Beck disease",
author="An Zhang, Jun-ling Cao, Bo Yang, Jing-hong Chen, Zeng-tie Zhang, Si-yuan Li, Qiang Fu, Clare E. Hugnes, Bruce Caterson",
journal="Journal of Zhejiang University Science B",
volume="11",
number="3",
pages="200-208",
year="2010",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0900074"
}

%0 Journal Article
%T Effects of moniliformin and selenium on human articular cartilage metabolism and their potential relationships to the pathogenesis of Kashin-Beck disease
%A An Zhang
%A Jun-ling Cao
%A Bo Yang
%A Jing-hong Chen
%A Zeng-tie Zhang
%A Si-yuan Li
%A Qiang Fu
%A Clare E. Hugnes
%A Bruce Caterson
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 3
%P 200-208
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0900074

TY - JOUR
T1 - Effects of moniliformin and selenium on human articular cartilage metabolism and their potential relationships to the pathogenesis of Kashin-Beck disease
A1 - An Zhang
A1 - Jun-ling Cao
A1 - Bo Yang
A1 - Jing-hong Chen
A1 - Zeng-tie Zhang
A1 - Si-yuan Li
A1 - Qiang Fu
A1 - Clare E. Hugnes
A1 - Bruce Caterson
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 3
SP - 200
EP - 208
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0900074


Abstract: 
Objective: To investigate the effects of mycotoxin moniliformin (MON) on the metabolism of aggrecan and type II collagen in human chondrocytes in vitro and the relationship between MON and kashin-Beck disease (KBD). Methods: Human chondrocytes were isolated and cultured on bone matrix gelatin to form an artificial cartilage model in vitro with or without MON toxin. Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The expression of aggrecan and type II collagen in the cartilage was determined using immunocytochemical staining. Results: MON toxin inhibited chondrocyte viability in dose-dependent and time-dependent manners. MON reduced aggrecan and type II collagen syntheses in the tissue-engineered cartilage. MON also increased the expression of matrix metalloproteinase-1 (MMP-1), MMP-13, BC4 epitopes, and CD44 in cartilages. However, the expression of 3B3(−) epitopes in cartilages was inhibited by MON. selenium partially alleviated the damage of aggrecan induced by MON toxin. Conclusion: MON toxin promoted the catabolism of aggrecan and type II collagen in human chondrocytes.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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