CLC number: R684
On-line Access: 2010-03-10
Received: 2009-03-12
Revision Accepted: 2009-12-29
Crosschecked: 2010-02-03
Cited: 15
Clicked: 6116
An Zhang, Jun-ling Cao, Bo Yang, Jing-hong Chen, Zeng-tie Zhang, Si-yuan Li, Qiang Fu, Clare E. Hugnes, Bruce Caterson. Effects of moniliformin and selenium on human articular cartilage metabolism and their potential relationships to the pathogenesis of Kashin-Beck disease[J]. Journal of Zhejiang University Science B, 2010, 11(3): 200-208.
@article{title="Effects of moniliformin and selenium on human articular cartilage metabolism and their potential relationships to the pathogenesis of Kashin-Beck disease",
author="An Zhang, Jun-ling Cao, Bo Yang, Jing-hong Chen, Zeng-tie Zhang, Si-yuan Li, Qiang Fu, Clare E. Hugnes, Bruce Caterson",
journal="Journal of Zhejiang University Science B",
volume="11",
number="3",
pages="200-208",
year="2010",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0900074"
}
%0 Journal Article
%T Effects of moniliformin and selenium on human articular cartilage metabolism and their potential relationships to the pathogenesis of Kashin-Beck disease
%A An Zhang
%A Jun-ling Cao
%A Bo Yang
%A Jing-hong Chen
%A Zeng-tie Zhang
%A Si-yuan Li
%A Qiang Fu
%A Clare E. Hugnes
%A Bruce Caterson
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 3
%P 200-208
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0900074
TY - JOUR
T1 - Effects of moniliformin and selenium on human articular cartilage metabolism and their potential relationships to the pathogenesis of Kashin-Beck disease
A1 - An Zhang
A1 - Jun-ling Cao
A1 - Bo Yang
A1 - Jing-hong Chen
A1 - Zeng-tie Zhang
A1 - Si-yuan Li
A1 - Qiang Fu
A1 - Clare E. Hugnes
A1 - Bruce Caterson
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 3
SP - 200
EP - 208
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0900074
Abstract: Objective: To investigate the effects of mycotoxin moniliformin (MON) on the metabolism of aggrecan and type II collagen in human chondrocytes in vitro and the relationship between MON and kashin-Beck disease (KBD). Methods: Human chondrocytes were isolated and cultured on bone matrix gelatin to form an artificial cartilage model in vitro with or without MON toxin. Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The expression of aggrecan and type II collagen in the cartilage was determined using immunocytochemical staining. Results: MON toxin inhibited chondrocyte viability in dose-dependent and time-dependent manners. MON reduced aggrecan and type II collagen syntheses in the tissue-engineered cartilage. MON also increased the expression of matrix metalloproteinase-1 (MMP-1), MMP-13, BC4 epitopes, and CD44 in cartilages. However, the expression of 3B3(−) epitopes in cartilages was inhibited by MON. selenium partially alleviated the damage of aggrecan induced by MON toxin. Conclusion: MON toxin promoted the catabolism of aggrecan and type II collagen in human chondrocytes.
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