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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.1 P.22-33


Promotion of the articular cartilage proteoglycan degradation by T-2 toxin and selenium protective effect

Author(s):  Si-yuan LI, Jun-ling CAO, Zhong-li SHI, Jing-hong CHEN, Zeng-tie ZHANG, Clare E. HUGHES, Bruce CATERSON

Affiliation(s):  Institute of Endemic Diseases, College of Medicine, Xi’ more

Corresponding email(s):   caojl@mail.xjtu.edu.cn, caterson@cardiff.ac.uk

Key Words:  T-2 toxin, Kashin-Beck disease (KBD), Aggrecan, IL-1&beta

Si-yuan LI, Jun-ling CAO, Zhong-li SHI, Jing-hong CHEN, Zeng-tie ZHANG, Clare E. HUGHES, Bruce CATERSON. Promotion of the articular cartilage proteoglycan degradation by T-2 toxin and selenium protective effect[J]. Journal of Zhejiang University Science B, 2008, 9(1): 22-33.

@article{title="Promotion of the articular cartilage proteoglycan degradation by T-2 toxin and selenium protective effect",
author="Si-yuan LI, Jun-ling CAO, Zhong-li SHI, Jing-hong CHEN, Zeng-tie ZHANG, Clare E. HUGHES, Bruce CATERSON",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Promotion of the articular cartilage proteoglycan degradation by T-2 toxin and selenium protective effect
%A Si-yuan LI
%A Jun-ling CAO
%A Zhong-li SHI
%A Jing-hong CHEN
%A Zeng-tie ZHANG
%A Clare E. HUGHES
%J Journal of Zhejiang University SCIENCE B
%V 9
%N 1
%P 22-33
%@ 1673-1581
%D 2008
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B071322

T1 - Promotion of the articular cartilage proteoglycan degradation by T-2 toxin and selenium protective effect
A1 - Si-yuan LI
A1 - Jun-ling CAO
A1 - Zhong-li SHI
A1 - Jing-hong CHEN
A1 - Zeng-tie ZHANG
A1 - Clare E. HUGHES
J0 - Journal of Zhejiang University Science B
VL - 9
IS - 1
SP - 22
EP - 33
%@ 1673-1581
Y1 - 2008
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B071322

Objective: To identify the relationship between t-2 toxin and kashin-Beck disease (KBD), the effects of t-2 toxin on aggrecan metabolism in human chondrocytes and cartilage were investigated in vitro. Methods: Chondrocytes were isolated from human articular cartilage and cultured in vitro. hyaluronic acid (HA), soluble CD44 (sCD44), IL-1&beta; and TNF-&alpha; levels in supernatants were measured by enzyme-linked immunosorbent assay (ELISA). CD44 content in chondrocyte membrane was determined by flow cytometry (FCM). CD44, hyaluronic acid synthetase-2 (HAS-2) and aggrecanases mRNA levels in chondrocytes were determined using reverse transcription polymerase chain reaction (RT-PCR). Immunocytochemical method was used to investigate expressions of BC-13, 3-B-3(−) and 2-B-6 epitopes in the cartilage reconstructed in vitro. Results: t-2 toxin inhibited CD44, HAS-2, and aggrecan mRNA expressions, but promoted aggrecanase-2 mRNA expression. Meanwhile, CD44 expression was found to be the lowest in the chondrocytes cultured with t-2 toxin and the highest in control plus selenium group. In addition, ELISA results indicated that there were higher sCD44, IL-1&beta; and TNF-&alpha; levels in t-2 toxin group. Similarly, higher HA levels were also observed in t-2 toxin group using radioimmunoprecipitation assay (RIPA). Furthermore, using monoclonal antibodies BC-13, 3-B-3 and 2-B-6, strong positive immunostaining was found in the reconstructed cartilage cultured with t-2 toxin, whereas no positive staining or very weak staining was observed in the cartilage cultured without t-2 toxin. Selenium could partly inhibit the effects of t-2 toxin above. Conclusion: t-2 toxin could inhibit aggrecan synthesis, promote aggrecanases and pro-inflammatory cytokines production, and consequently induce aggrecan degradation in chondrocytes. These will perturb metabolism balance between aggrecan synthesis and degradation in cartilage, inducing aggrecan loss in the end, which may be the initiation of the cartilage degradation.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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