CLC number: R318.08
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2015-10-20
Cited: 3
Clicked: 4671
Citations: Bibtex RefMan EndNote GB/T7714
Ming Zhao, Zhu Chen, Kang Liu, Yu-qing Wan, Xu-dong Li, Xu-wei Luo, Yi-guang Bai, Ze-long Yang, Gang Feng. Repair of articular cartilage defects in rabbits through tissue-engineered cartilage constructed with chitosan hydrogel and chondrocytes[J]. Journal of Zhejiang University Science B, 2015, 16(11): 914-923.
@article{title="Repair of articular cartilage defects in rabbits through tissue-engineered cartilage constructed with chitosan hydrogel and chondrocytes",
author="Ming Zhao, Zhu Chen, Kang Liu, Yu-qing Wan, Xu-dong Li, Xu-wei Luo, Yi-guang Bai, Ze-long Yang, Gang Feng",
journal="Journal of Zhejiang University Science B",
volume="16",
number="11",
pages="914-923",
year="2015",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1500036"
}
%0 Journal Article
%T Repair of articular cartilage defects in rabbits through tissue-engineered cartilage constructed with chitosan hydrogel and chondrocytes
%A Ming Zhao
%A Zhu Chen
%A Kang Liu
%A Yu-qing Wan
%A Xu-dong Li
%A Xu-wei Luo
%A Yi-guang Bai
%A Ze-long Yang
%A Gang Feng
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 11
%P 914-923
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500036
TY - JOUR
T1 - Repair of articular cartilage defects in rabbits through tissue-engineered cartilage constructed with chitosan hydrogel and chondrocytes
A1 - Ming Zhao
A1 - Zhu Chen
A1 - Kang Liu
A1 - Yu-qing Wan
A1 - Xu-dong Li
A1 - Xu-wei Luo
A1 - Yi-guang Bai
A1 - Ze-long Yang
A1 - Gang Feng
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 11
SP - 914
EP - 923
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500036
Abstract: Objective: In our previous work, we prepared a type of chitosan hydrogel with excellent biocompatibility. In this study, tissue-engineered cartilage constructed with this chitosan hydrogel and costal chondrocytes was used to repair the articular cartilage defects. Methods: chitosan hydrogels were prepared with a crosslinker formed by combining 1,6-diisocyanatohexane and polyethylene glycol. chitosan hydrogel scaffold was seeded with rabbit chondrocytes that had been cultured for one week in vitro to form the preliminary tissue-engineered cartilage. This preliminary tissue-engineered cartilage was then transplanted into the defective rabbit articular cartilage. There were three treatment groups: the experimental group received preliminary tissue-engineered cartilage; the blank group received pure chitosan hydrogels; and, the control group had received no implantation. The knee joints were harvested at predetermined time. The repaired cartilage was analyzed through gross morphology, histologically and immunohistochemically. The repairs were scored according to the international cartilage repair society (ICRS) standard. Results: The gross morphology results suggested that the defects were repaired completely in the experimental group after twelve weeks. The regenerated tissue connected closely with subchondral bone and the boundary with normal tissue was fuzzy. The cartilage lacuna in the regenerated tissue was similar to normal cartilage lacuna. The results of ICRS gross and histological grading showed that there were significant differences among the three groups (P<0.05). Conclusions: Chondrocytes implanted in the scaffold can adhere, proliferate, and secrete extracellular matrix. The novel tissue-engineered cartilage constructed in our research can completely repair the structure of damaged articular cartilage.
[1]Bettinger, C.J., 2011. Biodegradable elastomers for tissue engineering and cell-biomaterial interactions. Macromol. Biosci., 11(4):467-482.
[2]Brittberg, M., Peterson, L., 1998. Introduction of an articular cartilage classification. ICRS Newsletter., 1:5-8.
[3]Brittberg, M., Lindahl, A., Nilsson, A., et al., 1994. Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation. N. Engl. J. Med., 331(14):889-895.
[4]Broom, N.D., Oloyede, A., 1998. The importance of physicochemical swelling in cartilage illustrated with a model hydrgel system. Biomaterials, 19(13):1179-1188.
[5]Chen, Z., Zhao, M., Liu, K., et al., 2014. Novel chitosan hydrogel formed by ethylene glycol chitosan, 1,6-diisocyanatohexan and polyethylene glycol-400 for tissue engineering scaffold: in vitro and in vivo evaluation. J. Mater. Sci.: Mater. Med., 25(8):1903-1913.
[6]de Franceschi, L., Grigolo, B., Roseti, L., et al., 2005. Transplantation of chondrocytes seeded on collagen-based scaffold in cartilage defects in rabbits. J. Biomed. Mater. Res. A, 75(3):612-622.
[7]Dzioba, R.B., 1988. The classification and treatment of acute articular cartilage lesions. Arthroscopy, 4(2):72-80.
[8]Fong, E.L., Watson, B.M., Kasper, F.K., et al., 2012. Building bridges: leveraging interdisciplinary collaborations in the development of biomaterials to meet clinical needs. Adv. Mater., 24(36):4995-5013.
[9]Francis Suh, J.K., Matthew, H.W., 2000. Application of chitosan-based polysaccharide biomaterials in cartilage tissue engineering: a review. Biomaterials, 21(24):2589-2598.
[10]Gentili, G., Cancedda, R., 2009. Cartilage and bone extracellular matrix. Curr. Pharm. Design, 15(12):1334-1348.
[11]Hao, T., Wen, N., Cao, J.K., et al., 2010. The support of matrix accumulation and the promotion of sheep articular cartilage defects repair in vivo by chitosan hydrogels. Osteoarthritis Cartilage, 18(2):257-265.
[12]Holmes, J.S., 2004. Articular cartilage injuries in the athlete’s knee: current concepts in diagnosis and treatment. South. Med. J., 97(8):742-747.
[13]Iwasa, J., Engebretsen, L., Shima, Y., 2009. Clinical application of scaffolds for cartilage tissue engineering. Knee Surg. Sports Traumatol. Arthrosc., 17(6):561-577.
[14]Jin, R., Moreira Teixeira, L.S., Dijkstra, P.J., et al., 2009. Injectable chitosan-based hydrogels for cartilage tissue engineering. Biomaterials, 30(13):2544-2551.
[15]Kim, I.Y., Seo, S.J., Moon, H.S., et al., 2008. Chitosan and its derivatives for tissue engineering applications. Biotechnol. Adv., 26(1):1-21.
[16]Kosuge, D., Khan, W.S., Haddad, B., et al., 2013. Biomaterials and scaffolds in bone and musculoskeletal engineering. Curr. Stem Cell Res. Ther., 8(3):185-191.
[17]Kuo, Y.C., Lin, C.Y., 2006. Effect of genipin-crosslinked chitin-chitosan scaffolds with hydroxyapatite modifications on the cultivation of bovine knee chondrocytes. Biotechnol. Bioeng., 95(1):132-144.
[18]Lee, K.Y., Mooney, D.J., 2001. Hydrogels for tissue engineering. Chem. Rev., 101(7):1869-1879.
[19]Liu, J., Wu, D.Y., Wang, T., 2010. Research progress in antibacterial activity of chitosan and development of antimicrobial textile. J. Textile Res., 31(7):146-150.
[20]Mainil-Varlet, P., Aigner, T., Brittberg, M., et al., 2003. Histological assessment of cartilage repair: a report by the Histology Endpoint Committee of the International Cartilage Repair Society (ICRS). J. Bone Joint Surg. Am., 85-A(Suppl. 2):45-57.
[21]Mallick, K.K., Cox, S.C., 2013. Biomaterial scaffolds for tissue engineering. Front. Biosci., 5:341-360.
[22]Messent, E.A., Ward, R.J., Tonkin, C.J., et al., 2007. Osteophytes, juxta-articular radiolucencies and cancellous bone changes in the proximal tibia of patients with knee osteoarthritis. Osteoarthritis Cartilage, 15(2):179-186.
[23]Nettles, D.L., Elder, S.H., Gilbert, J.A., 2002. Potential use of chitosan as a cell scaffold material for cartilage tissue engineering. Tissue Eng., 8(6):1009-1016.
[24]Ochi, M., Uchio, Y., Kawasaki, K., et al., 2002. Transplantation of cartilage-like tissue made by tissue engineering in the treatment of cartilage defect of the knee. J. Bone Joint Surg. Br., 84(4):571-578.
[25]Perka, C., Spitzer, R.S., Lindenhayn, K., et al., 2000. Matrix-mixed culture: new methodology for chondrocyte culture and preparation of cartilage transplants. J. Biomed. Mater. Res., 49(3):305-311.
[26]Peterson, L., Minas, T., Brittberg, M., et al., 2000. Two- to 9-year outcome after autologous chondrocyte transplantation of the knee. Clin. Orthop. Relat. Res., 374:212-234.
[27]Peterson, L., Vasiliadis, H.S., Brttiberg, M., et al., 2010. Autologous chondrycyte implantation a long-term follow up. Am. J. Sports Med., 38(6):1117-1124.
[28]Qiao, P.Y., Li, F.F., Dong, L.M., et al., 2014. Delivering MC3T3-E1 cells into injectable calcium phosphate cement through alginate-chitosan microcapsules for bone tissue engineering. J. Zhejiang Univ.-Sci. B (Biomed. & Biotechnol.), 15(4):382-392.
[29]Redman, S.N., Oldfield, S.F., Archer, C.W., 2005. Current strategies for articular cartilage repair. Eur. Cell Mater., 9:23-32.
[30]Richter, W., 2009. Mesenchymal stem cells and cartilage in situ regeneration. J. Intern. Med., 266(4):390-405.
[31]Roach, H.I., Aigner, T., Soder, S., et al., 2007. Pathobiology of osteoarthritis: pathomechanisms and potential therapeutic targets. Curr. Drug Targets, 8(2):271-282.
[32]Steadman, J.R., Rodkey, W.G., Briggs, K.K., 2002. Microfracture to treat full-thickness chondral defects: surgical technique, rehabilitation, and outcomes. J. Knee Surg., 15(3):170-176.
[33]Wakitani, S., Goto, T., Pineda, S.J., et al., 1994. Mesenchymal cell-based repair of large, full-thickness defects of articular cartilage. J. Bone Joint Surg. Am., 76(4):579-592.
[34]Wakitani, S., Goto, T., Young, R.G., et al., 1998. Repair of large full-thickness articular cartilage defects with allograft articular chondrocytes embedded in a collagen gel. Tissue Eng., 4(4):429-444.
[35]Willers, C., Partsalis, T., Zheng, M.H., 2007. Articular cartilage repair: procedures versus products. Expert Rev. Med. Devices, 4(3):373-392.
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