Full Text:   <1957>

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CLC number: R318.08

On-line Access: 2015-11-04

Received: 2015-02-04

Revision Accepted: 2015-06-09

Crosschecked: 2015-10-20

Cited: 3

Clicked: 3317

Citations:  Bibtex RefMan EndNote GB/T7714


Ming Zhao


Gang Feng


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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.11 P.914-923


Repair of articular cartilage defects in rabbits through tissue-engineered cartilage constructed with chitosan hydrogel and chondrocytes

Author(s):  Ming Zhao, Zhu Chen, Kang Liu, Yu-qing Wan, Xu-dong Li, Xu-wei Luo, Yi-guang Bai, Ze-long Yang, Gang Feng

Affiliation(s):  Institute of Tissue Engineering and Stem Cells, Nanchong Central Hospital and the Second Clinical Institute of North Sichuan Medical University, Nanchong 637000, China; more

Corresponding email(s):   fenggangncch@163.com

Key Words:  Articular cartilage, Chitosan hydrogel, Repair, Tissue engineering

Ming Zhao, Zhu Chen, Kang Liu, Yu-qing Wan, Xu-dong Li, Xu-wei Luo, Yi-guang Bai, Ze-long Yang, Gang Feng. Repair of articular cartilage defects in rabbits through tissue-engineered cartilage constructed with chitosan hydrogel and chondrocytes[J]. Journal of Zhejiang University Science B, 2015, 16(11): 914-923.

@article{title="Repair of articular cartilage defects in rabbits through tissue-engineered cartilage constructed with chitosan hydrogel and chondrocytes",
author="Ming Zhao, Zhu Chen, Kang Liu, Yu-qing Wan, Xu-dong Li, Xu-wei Luo, Yi-guang Bai, Ze-long Yang, Gang Feng",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Repair of articular cartilage defects in rabbits through tissue-engineered cartilage constructed with chitosan hydrogel and chondrocytes
%A Ming Zhao
%A Zhu Chen
%A Kang Liu
%A Yu-qing Wan
%A Xu-dong Li
%A Xu-wei Luo
%A Yi-guang Bai
%A Ze-long Yang
%A Gang Feng
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 11
%P 914-923
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500036

T1 - Repair of articular cartilage defects in rabbits through tissue-engineered cartilage constructed with chitosan hydrogel and chondrocytes
A1 - Ming Zhao
A1 - Zhu Chen
A1 - Kang Liu
A1 - Yu-qing Wan
A1 - Xu-dong Li
A1 - Xu-wei Luo
A1 - Yi-guang Bai
A1 - Ze-long Yang
A1 - Gang Feng
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 11
SP - 914
EP - 923
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500036

Objective: In our previous work, we prepared a type of chitosan hydrogel with excellent biocompatibility. In this study, tissue-engineered cartilage constructed with this chitosan hydrogel and costal chondrocytes was used to repair the articular cartilage defects. Methods: chitosan hydrogels were prepared with a crosslinker formed by combining 1,6-diisocyanatohexane and polyethylene glycol. chitosan hydrogel scaffold was seeded with rabbit chondrocytes that had been cultured for one week in vitro to form the preliminary tissue-engineered cartilage. This preliminary tissue-engineered cartilage was then transplanted into the defective rabbit articular cartilage. There were three treatment groups: the experimental group received preliminary tissue-engineered cartilage; the blank group received pure chitosan hydrogels; and, the control group had received no implantation. The knee joints were harvested at predetermined time. The repaired cartilage was analyzed through gross morphology, histologically and immunohistochemically. The repairs were scored according to the international cartilage repair society (ICRS) standard. Results: The gross morphology results suggested that the defects were repaired completely in the experimental group after twelve weeks. The regenerated tissue connected closely with subchondral bone and the boundary with normal tissue was fuzzy. The cartilage lacuna in the regenerated tissue was similar to normal cartilage lacuna. The results of ICRS gross and histological grading showed that there were significant differences among the three groups (P<0.05). Conclusions: Chondrocytes implanted in the scaffold can adhere, proliferate, and secrete extracellular matrix. The novel tissue-engineered cartilage constructed in our research can completely repair the structure of damaged articular cartilage.




Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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