Full Text:   <1957>

CLC number: Q946.91+4

On-line Access: 2012-06-29

Received: 2012-02-19

Revision Accepted: 2012-05-23

Crosschecked: 2012-09-10

Cited: 4

Clicked: 3776

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE B 2012 Vol.13 No.10 P.824-830


Determination of the geographical origin of Chinese teas based on stable carbon and nitrogen isotope ratios

Author(s):  Long Zhang, Jia-rong Pan, Cheng Zhu

Affiliation(s):  College of Life Sciences, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   panjr@263.net, pzhch@cjlu.edu.cn

Key Words:  Geographical origin, Stable carbon isotope ratios, Stable nitrogen isotope ratios, Tea

Long Zhang, Jia-rong Pan, Cheng Zhu. Determination of the geographical origin of Chinese teas based on stable carbon and nitrogen isotope ratios[J]. Journal of Zhejiang University Science B, 2012, 13(10): 824-830.

@article{title="Determination of the geographical origin of Chinese teas based on stable carbon and nitrogen isotope ratios",
author="Long Zhang, Jia-rong Pan, Cheng Zhu",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Determination of the geographical origin of Chinese teas based on stable carbon and nitrogen isotope ratios
%A Long Zhang
%A Jia-rong Pan
%A Cheng Zhu
%J Journal of Zhejiang University SCIENCE B
%V 13
%N 10
%P 824-830
%@ 1673-1581
%D 2012
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200046

T1 - Determination of the geographical origin of Chinese teas based on stable carbon and nitrogen isotope ratios
A1 - Long Zhang
A1 - Jia-rong Pan
A1 - Cheng Zhu
J0 - Journal of Zhejiang University Science B
VL - 13
IS - 10
SP - 824
EP - 830
%@ 1673-1581
Y1 - 2012
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1200046

The objective of this study was to investigate the geographical origin of Chinese teas using carbon and nitrogen stable isotope ratio technology. The results showed that inter-provincial dispersion of teas in Guangdong (GD), Guangxi (GX), Hainan (HA), Fujian (FJ), Shandong (SD), Sichuan (SC), Chongqing (CQ), and Henan (HN) provinces was high, while in Zhejiang (ZJ), Hubei (HB), Yunnan (YN), and Anhui (AH) provinces, it was low. tea samples from GD, GX, HA, and FJ provinces were clustered in one group and separated from those from AH and HB provinces. Thus, carbon and nitrogen stable isotope ratio technology could discriminate teas from among some provinces of China, but not from among others. Better separation might be obtained with a combination of isotopic ratios and other indexes, such as elemental data and organic components.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1]Angerosa, F., Camera, L., Cumitini, S., Gleixner, G., Reniero, F., 1997. Carbon stable isotopes and olive oil adulteration with pomace oil. J. Agric. Food Chem., 45(8):3044-3048.

[2]Antolovich, M., Li, X., Robards, K., 2001. Detection of adulteration in Australian orange juices by stable carbon isotope ratio analysis (SCIRA). J. Agric. Food Chem., 49(5):2623-2626.

[3]Bong, Y.S., Shin, W.J., Lee, A.R., Kim, Y.S., Kim, K., Lee, K.S., 2010. Tracing the geographical origin of beefs being circulated in Korean markets based on stable isotopes. Rapid Commun. Mass Spectrom., 24(1):155-159.

[4]Camin, F., Bontempo, L., Heinrich, K., Horacek, M., Kelly, S.D., Schlicht, C., Thomas, F., Monahan, F.J., Hoogewerff, J., Rossmann, A., 2007. Multi-element (H, C, N, S) stable isotope characteristics of lamb meat from different European regions. Anal. Bioanal. Chem., 389(1):309-320.

[5]Camin, F., Larcher, R., Nicolini, G., Bontempo, L., Bertoldi, D., Perini, M., Schlicht, C., Schellenberg, A., Thomas, F., Heinrich, K., et al., 2010. Isotopic and elemental data for tracing the origin of European olive oils. J. Agric. Food Chem., 58(1):570-577.

[6]Cyr, D.R., Bewley, J.D., 1989. Carbon and nitrogen reserves of leafy spurge (Euphorbia esula) roots as related to overwintering strategy. Physiol. Plant., 77(1):67-72.

[7]Diaz, M., Haag-Kerwer, A., Wingfield, R., Ball, E., Olivares, E., Grams, T.E.E., Ziegler, H., Lüttge, U., 1996. Relationships between carbon and hydrogen isotope ratios and nitrogen levels of Clusia species and two other Clusiaceae genera at various sites and different altitudes in Venezuela. Trees, 10(6):351-358.

[8]Ehleringer, J.R., Casale, J., Cooper, D.A., Lott, M.J., 2001. Sourcing drugs with stable isotopes. NeuroImage, 84112(801):1080-1094.

[9]Farquhar, G.D., 1983. On the nature of carbon isotope discrimination in C4 species. Aust. J. Plant Physiol., 10(2):205-226.

[10]Farquhar, G.D., O′Leary, M.H., Berry, J.A., 1982. On the relationship between carbon isotope discrimination and the intercellular carbon dioxide concentration in leaves. Aust. J. Plant Physiol., 9(2):121-137.

[11]González-Martin, I., Gonzalez-Perez, C., Hernandez, M.J., Marques-Macias, E., Sanz-Poveda, F.S., 1999. Use of isotope analysis to characterize meat from Iberian-breed swine. Meat Sci., 52(4):437-441.

[12]Gremaud, G., Hilkert, A., 2009. Isotopic-Spectroscopic Techniques: Stable Isotope Ratio Mass Spectroscopy (IRMS). In: Sun, D.W. (Ed.), Modern Techniques for Food Authentication. Academic Press, New York, p.269-320.

[13]Guo, B.L., Wei, Y.M., Simon, K.D., Pan, J.R., Wei, S., 2009. Application of stable hydrogen isotope analysis in beef geographical origin traceability. Chin. J. Anal. Chem., 37(9):1333-1336.

[14]Guo, B.L., Wei, Y.M., Pan, J.R., Li, Y., 2010. Stable C and N isotope ratio analysis for regional geographical traceability of cattle in China. Food Chem., 118(4):915-920.

[15]Heaton, K., Kelly, S.D., Hoogewerff, J., Woolfe, M., 2008. Verifying the geographical origin of beef: the application of multi-element isotope and trace element analysis. Food Chem., 107(1):506-515.

[16]Heaton, T.H.E., 1987. The 15N/14N ratios of plants in South Africa and Namibia: relationship to climate and coastal/ saline environments. Oecologia (Berlin), 74(2):236-246.

[17]Horacek, M., Min, J.S., 2010. Discrimination of Korean beef from beef of other origin by stable isotope measurements. Food Chem., 121(2):517-520.

[18]Kelly, S., Baxter, M., Chapman, S., Rhodes, C., Dennis, J., Brereton, P., 2002. The application of isotopic and elemental analysis to determine the geographical origin of premium long grain rice. Eur. Food Res. Technol., 214(1):72-78.

[19]Kohl, D.H., Shearer, G.B., Commones, B., 1973. Variation of 15N in corn and soil following applications of fertilizer nitrogen. Soil Sci. Soc. Am. J., 37(6):888-892.

[20]Kropf, U., Golob, T., Nečemer, M., Kump, P., Korošec, M., Bertoncelj, J., Ogrinc, N., 2010. Carbon and nitrogen natural stable isotopes in Slovene honey: adulteration and botanical and geographical aspects. J. Agric. Food Chem., 58(24):12794-12803.

[21]Longobardi, F., Casiello, G., Sacco, D., Tedone, L., Sacco, A., 2011. Characterisation of the geographical origin of Italian potatoes, based on stable isotope and volatile compound analyses. Food Chem., 124(4):1708-1713.

[22]Moing, A., Lafargue, B., Lespinasse, J.M., Gaudillère, J.P., 1994. Carbon and nitrogen reserves in prune tree shoots: effect of training system. Sci. Hort., 57(1-2):99-110.

[23]Moore, D.S., McCabe, G.P., 1993. Introduction to the Practice of Statistics, 5th Ed. W.H. Freeman and Company, New York, p.191-250.

[24]Nakashita, R., Suzuki, Y., Akamatsu, F., Iizumi, Y., Korenaga, T., Chikaraishi, Y., 2008. Stable carbon, nitrogen, and oxygen isotope analysis as a potential tool for verifying geographical origin of beef. Anal. Chim. Acta, 617(1-2):148-152.

[25]Padovan, G.J., de Jong, D., Rodrigues, L.P., Marchini, J.S., 2003. Detection of adulteration of commercial honey samples by the 13C/12C isotopic ratio. Food Chem., 82(4):633-636.

[26]Pilgrim, T.S., Watling, R.J., Grice, K., 2010. Application of trace element and stable isotope signatures to determine the provenance of tea (Camellia sinensis) samples. Food Chem., 118(4):921-926.

[27]Roßmann, A., Schmidt, H.L., Reniero, F., Versini, G., Moussa, I., Merle, M.H., 1996. Stable carbon isotope content in ethanol of EC data bank wines from Italy, France and Germany. Z. Lebensm. Unters. Forsch., 203(3):293-301.

[28]Schellenberg, A., Chmielus, S., Schlicht, C., Camin, F., Perini, M., Bontempo, L., Heinrich, K., Kelly, S.D., Rossmann, A., Thomas, F., et al., 2010. Multielement stable isotope ratios (H, C, N, S) of honey from different European regions. Food Chem., 121(3):770-777.

[29]Shearer, G., Legg, J.O., 1975. Variation in the natural abundance of 15N of wheat plants in relation to fertilizer nitrogen applications. Soil Sci. Soc. Am. J., 39(5):896-901.

[30]Shibuya, E.K., Sarkis, J.E.S., Negrini-Neto, O., Martinelli, L.A., 2007. Carbon and nitrogen stable isotopes as indicative of geographical origin of marijuana samples seized in the city of São Paulo (Brazil). Forensic Sci. Int., 167(1):8-15.

[31]Sun, S.M., Guo, B.L., Wei, Y.M., Fan, M.T., 2010. Analysis of stable carbon and nitrogen isotope compositions and geographical origins of sheep tissues. Sci. Agric. Sin., 43(8):1670-1676.

[32]Suzuki, Y., Chikaraishi, Y., Ogawa, N.O., Ohkouchi, N., Korenag, T., 2008. Geographical origin of polished rice based on multiple element and stable isotope analyses. Food Chem., 109(2):470-475.

[33]Ta, T.C., Macdowall, F.D.H., Faris, M.A., 1990. Utilization of carbon and nitrogen reserves of alfalfa roots in supporting N2-fixation and shoot regrowth. Plant Soil, 127(2):231-236.

[34]Weber, D., Roßmann, A., Schwarz, S., Schmidt, H.L., 1997. Correlations of carbon isotope ratios of wine ingredients for the improved detection of adulterations. I. Organic acids and ethanol. Z. Lebensm. Unters. Forsch., 205(2):158-164.

Open peer comments: Debate/Discuss/Question/Opinion


Please provide your name, email address and a comment

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2022 Journal of Zhejiang University-SCIENCE