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CLC number: S685.21

On-line Access: 2013-04-03

Received: 2012-03-26

Revision Accepted: 2012-09-27

Crosschecked: 2013-03-01

Cited: 3

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Citations:  Bibtex RefMan EndNote GB/T7714

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Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.4 P.299-308


Determination of genetic relationships between evergreen azalea cultivars in China using AFLP markers

Author(s):  Hong Zhou, Jin Liao, Yi-ping Xia, Yuan-wen Teng

Affiliation(s):  Department of Horticulture, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China

Corresponding email(s):   ypxia@zju.edu.cn, ywteng@zju.edu.cn

Key Words:  Rhododendron spp., Tsutsusi, Fluorescent label, Dice coefficient, Principal coordinate analysis (PCO), Neighbor joining tree

Hong Zhou, Jin Liao, Yi-ping Xia, Yuan-wen Teng. Determination of genetic relationships between evergreen azalea cultivars in China using AFLP markers[J]. Journal of Zhejiang University Science B, 2013, 14(4): 299-308.

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author="Hong Zhou, Jin Liao, Yi-ping Xia, Yuan-wen Teng",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

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%T Determination of genetic relationships between evergreen azalea cultivars in China using AFLP markers
%A Hong Zhou
%A Jin Liao
%A Yi-ping Xia
%A Yuan-wen Teng
%J Journal of Zhejiang University SCIENCE B
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%N 4
%P 299-308
%@ 1673-1581
%D 2013
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200094

T1 - Determination of genetic relationships between evergreen azalea cultivars in China using AFLP markers
A1 - Hong Zhou
A1 - Jin Liao
A1 - Yi-ping Xia
A1 - Yuan-wen Teng
J0 - Journal of Zhejiang University Science B
VL - 14
IS - 4
SP - 299
EP - 308
%@ 1673-1581
Y1 - 2013
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1200094

Evergreen azaleas are among the most important ornamental shrubs in China. Today, there are probably over 300 cultivars preserved in different nurseries, but with little information available on the cultivar itself or relationships between cultivars. Amplified fragment length polymorphism (AFLP) markers were employed to determine the genetic relationships between evergreen azalea cultivars in China. One hundred and thirty genotypes collected from gardens and nurseries, including cultivars classified in the groups East, West, Hairy, and Summer, unknown cultivars, and close species, were analyzed using three primer pairs. A total of 408 polymorphic fragments were generated by AFLP reactions with an average of 136 fragments per primer pair. The average values of expected heterozygosity and Shannon’s information index were 0.3395 and 0.5153, respectively. Genetic similarities were generated based on dice coefficients, used to construct a neighbor joining tree, and bootstrapped for 100 replicates in Treecon V1.3b. principal coordinate analysis (PCO) was performed based on Dice distances using NTSYS-pc software. The AFLP technique was useful for analyzing genetic diversity in evergreen azaleas. Cluster analysis revealed that cultivars in the West and Summer groups were quite distinct from other groups in the four-group classification system and that the East and Hairy groups should be redefined.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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