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CLC number: R378.99

On-line Access: 2013-06-03

Received: 2012-08-17

Revision Accepted: 2013-05-18

Crosschecked: 2013-08-06

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Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.9 P.837-843

http://doi.org/10.1631/jzus.B1200221


Influence of induced ciprofloxacin resistance on efflux pump activity of Klebsiella pneumoniae


Author(s):  Hai-qin Zhong, Shun Zhang, Hong Pan, Ting Cai

Affiliation(s):  Department of Internal Medicine, School of Medicine, Ningbo University, Ningbo 315211, China; more

Corresponding email(s):   caiting@nbu.edu.cn

Key Words:  Klebsiella pneumoniae, Efflux pump, Ciprofloxacin, Antibiotic resistance


Hai-qin Zhong, Shun Zhang, Hong Pan, Ting Cai. Influence of induced ciprofloxacin resistance on efflux pump activity of Klebsiella pneumoniae[J]. Journal of Zhejiang University Science B, 2013, 14(9): 837-843.

@article{title="Influence of induced ciprofloxacin resistance on efflux pump activity of Klebsiella pneumoniae",
author="Hai-qin Zhong, Shun Zhang, Hong Pan, Ting Cai",
journal="Journal of Zhejiang University Science B",
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number="9",
pages="837-843",
year="2013",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1200221"
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%0 Journal Article
%T Influence of induced ciprofloxacin resistance on efflux pump activity of Klebsiella pneumoniae
%A Hai-qin Zhong
%A Shun Zhang
%A Hong Pan
%A Ting Cai
%J Journal of Zhejiang University SCIENCE B
%V 14
%N 9
%P 837-843
%@ 1673-1581
%D 2013
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200221

TY - JOUR
T1 - Influence of induced ciprofloxacin resistance on efflux pump activity of Klebsiella pneumoniae
A1 - Hai-qin Zhong
A1 - Shun Zhang
A1 - Hong Pan
A1 - Ting Cai
J0 - Journal of Zhejiang University Science B
VL - 14
IS - 9
SP - 837
EP - 843
%@ 1673-1581
Y1 - 2013
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1200221


Abstract: 
Objective: The efflux pump (EP) is one of the major mechanisms of antibiotic resistance in Klebsiella pneumoniae. However, there are few reports on the effect of the abuse of antibiotic use on the activity of EPs. To determine whether the use of low efficacy antibiotics has any effect on the activity of EPs and induces drug resistance in K. pneumoniae, we investigated the effect of ciprofloxacin on the activity of EPs in K. pneumoniae strains. Methods: Sixteen susceptible K. pneumoniae strains were isolated from patients and their minimum inhibitory concentrations (MICs) of ciprofloxacin were measured in the absence and presence of the pump inhibitor carbonyl cyanide m-chlorophenyl hydrazone (CCCP). The strains were then induced with a gradient of ciprofloxacin until the MICs of the strains showed no further increase, to obtain induced resistant strains. The EP activities of the strains before and after induction were compared using EP inhibition and ethidium bromide (EtBr) accumulation assays. Results: The MIC values of the strains were 16‒256 times higher after induction than before induction. In the presence of CCCP, the MIC values of 50% of the induced strains were 2‒4-fold lower than that in the absence of this inhibitor. The EtBr accumulation assay showed that the fluorescence of EtBr in the induced cells was lower than that in the cells before induction. Conclusions: EPs are widespread in susceptible and drug-resistant K. pneumoniae strains. Induction with ciprofloxacin may increase the activity of EPs in K. pneumoniae. The EtBr accumulation assay is more sensitive than the EP inhibition assay in evaluating the activity of EPs in K. pneumoniae.

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