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CLC number: S432.1

On-line Access: 2014-01-28

Received: 2013-04-11

Revision Accepted: 2013-08-21

Crosschecked: 2014-01-19

Cited: 7

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Citations:  Bibtex RefMan EndNote GB/T7714

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Journal of Zhejiang University SCIENCE B 2014 Vol.15 No.2 P.133-142


Molecular variability and evolution of a natural population of tomato yellow leaf curl virus in Shanghai, China*

Author(s):  Xiu-ling Yang, Meng-ning Zhou, Ya-juan Qian, Yan Xie, Xue-ping Zhou

Affiliation(s):  . State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China

Corresponding email(s):   xiey@zju.edu.cn

Key Words:  Molecular variability, Tomato yellow leaf curl virus (TYLCV), Geminivirus, Evolution

Xiu-ling Yang, Meng-ning Zhou, Ya-juan Qian, Yan Xie, Xue-ping Zhou. Molecular variability and evolution of a natural population of tomato yellow leaf curl virus in Shanghai, China[J]. Journal of Zhejiang University Science B, 2014, 15(2): 133-142.

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author="Xiu-ling Yang, Meng-ning Zhou, Ya-juan Qian, Yan Xie, Xue-ping Zhou",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Molecular variability and evolution of a natural population of tomato yellow leaf curl virus in Shanghai, China
%A Xiu-ling Yang
%A Meng-ning Zhou
%A Ya-juan Qian
%A Yan Xie
%A Xue-ping Zhou
%J Journal of Zhejiang University SCIENCE B
%V 15
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%P 133-142
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%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1300110

T1 - Molecular variability and evolution of a natural population of tomato yellow leaf curl virus in Shanghai, China
A1 - Xiu-ling Yang
A1 - Meng-ning Zhou
A1 - Ya-juan Qian
A1 - Yan Xie
A1 - Xue-ping Zhou
J0 - Journal of Zhejiang University Science B
VL - 15
IS - 2
SP - 133
EP - 142
%@ 1673-1581
Y1 - 2014
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1300110

tomato yellow leaf curl virus (TYLCV), belonging to the genus Begomovirus of the family Geminiviridae, is emerging as the most destructive pathogen of tomato plants. Since the first report of TYLCV in Shanghai, China in 2006, TYLCV has spread rapidly to 13 provinces or autonomous regions of China. In this study, the molecular variability and evolution of TYLCV were monitored in Shanghai from its first upsurge in 2006 until 2010. Full-length genomic sequences of 26 isolates were obtained by rolling circle amplification. Sequence analysis showed that the intergenic region was the most variable, with a mean mutation rate of 4.81×10−3 nucleotide substitutions per site per year. Genetic differentiation was found within isolates obtained from 2006, 2009, and 2010, though a linear increase in genetic diversity over time was not evident. Whilst significant parts of TYLCV genes were under negative selection, the C4 gene embedded entirely within the C1 gene had a tendency to undergo positive selection. Our results indicate that a mechanism of independent evolution of overlapping regions could apply to the natural population of TYLCV in Shanghai, China.




Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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