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CLC number: S572

On-line Access: 2013-12-03

Received: 2013-05-06

Revision Accepted: 2013-07-04

Crosschecked: 2013-11-25

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Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.12 P.1100-1109

http://doi.org/10.1631/jzus.B1300130


Genetic variation in alkaloid accumulation in leaves of Nicotiana


Author(s):  Bo Sun, Fen Zhang, Guo-jun Zhou, Guo-hai Chu, Fang-fang Huang, Qiao-mei Wang, Li-feng Jin, Fu-cheng Lin, Jun Yang

Affiliation(s):  Zhengzhou Tobacco Research Institute, Zhengzhou 450001, China; more

Corresponding email(s):   yangjun@ztri.com.cn

Key Words:  Alkaloid, Genetic effects, Nicotiana


Bo Sun, Fen Zhang, Guo-jun Zhou, Guo-hai Chu, Fang-fang Huang, Qiao-mei Wang, Li-feng Jin, Fu-cheng Lin, Jun Yang. Genetic variation in alkaloid accumulation in leaves of Nicotiana[J]. Journal of Zhejiang University Science B, 2013, 14(12): 1100-1109.

@article{title="Genetic variation in alkaloid accumulation in leaves of Nicotiana",
author="Bo Sun, Fen Zhang, Guo-jun Zhou, Guo-hai Chu, Fang-fang Huang, Qiao-mei Wang, Li-feng Jin, Fu-cheng Lin, Jun Yang",
journal="Journal of Zhejiang University Science B",
volume="14",
number="12",
pages="1100-1109",
year="2013",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1300130"
}

%0 Journal Article
%T Genetic variation in alkaloid accumulation in leaves of Nicotiana
%A Bo Sun
%A Fen Zhang
%A Guo-jun Zhou
%A Guo-hai Chu
%A Fang-fang Huang
%A Qiao-mei Wang
%A Li-feng Jin
%A Fu-cheng Lin
%A Jun Yang
%J Journal of Zhejiang University SCIENCE B
%V 14
%N 12
%P 1100-1109
%@ 1673-1581
%D 2013
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1300130

TY - JOUR
T1 - Genetic variation in alkaloid accumulation in leaves of Nicotiana
A1 - Bo Sun
A1 - Fen Zhang
A1 - Guo-jun Zhou
A1 - Guo-hai Chu
A1 - Fang-fang Huang
A1 - Qiao-mei Wang
A1 - Li-feng Jin
A1 - Fu-cheng Lin
A1 - Jun Yang
J0 - Journal of Zhejiang University Science B
VL - 14
IS - 12
SP - 1100
EP - 1109
%@ 1673-1581
Y1 - 2013
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1300130


Abstract: 
alkaloids are plant secondary metabolites that are widely distributed in Nicotiana species and contribute greatly to the quality of tobacco leaves. Some alkaloids, such as nornicotine and myosmine, have adverse effects on human health. To reduce the content of harmful alkaloids in tobacco leaves through conventional breeding, a genetic study of the alkaloid variation among different genotypes is required. In this study, alkaloid profiles in leaves of five Nicotiana tabacum cultivars and Nicotiana tomentosiformis were investigated. Six alkaloids were identified from all six genotypes via gas chromatograph-mass spectrometry (GC-MS). Significant differences in alkaloid content were observed both among different leaf positions and among cultivars. The contents of nornicotine and myosmine were positively and significantly correlated (R2=0.881), and were also separated from those of other alkaloids by clustering. Thus, the genotype plays a major role in alkaloid accumulation, indicating a high potential for manipulation of alkaloid content through traditional breeding.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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