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CLC number: S572

On-line Access: 2013-12-03

Received: 2013-05-06

Revision Accepted: 2013-07-04

Crosschecked: 2013-11-25

Cited: 1

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Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.12 P.1100-1109


Genetic variation in alkaloid accumulation in leaves of Nicotiana

Author(s):  Bo Sun, Fen Zhang, Guo-jun Zhou, Guo-hai Chu, Fang-fang Huang, Qiao-mei Wang, Li-feng Jin, Fu-cheng Lin, Jun Yang

Affiliation(s):  Zhengzhou Tobacco Research Institute, Zhengzhou 450001, China; more

Corresponding email(s):   yangjun@ztri.com.cn

Key Words:  Alkaloid, Genetic effects, Nicotiana

Bo Sun, Fen Zhang, Guo-jun Zhou, Guo-hai Chu, Fang-fang Huang, Qiao-mei Wang, Li-feng Jin, Fu-cheng Lin, Jun Yang. Genetic variation in alkaloid accumulation in leaves of Nicotiana[J]. Journal of Zhejiang University Science B, 2013, 14(12): 1100-1109.

@article{title="Genetic variation in alkaloid accumulation in leaves of Nicotiana",
author="Bo Sun, Fen Zhang, Guo-jun Zhou, Guo-hai Chu, Fang-fang Huang, Qiao-mei Wang, Li-feng Jin, Fu-cheng Lin, Jun Yang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Genetic variation in alkaloid accumulation in leaves of Nicotiana
%A Bo Sun
%A Fen Zhang
%A Guo-jun Zhou
%A Guo-hai Chu
%A Fang-fang Huang
%A Qiao-mei Wang
%A Li-feng Jin
%A Fu-cheng Lin
%A Jun Yang
%J Journal of Zhejiang University SCIENCE B
%V 14
%N 12
%P 1100-1109
%@ 1673-1581
%D 2013
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1300130

T1 - Genetic variation in alkaloid accumulation in leaves of Nicotiana
A1 - Bo Sun
A1 - Fen Zhang
A1 - Guo-jun Zhou
A1 - Guo-hai Chu
A1 - Fang-fang Huang
A1 - Qiao-mei Wang
A1 - Li-feng Jin
A1 - Fu-cheng Lin
A1 - Jun Yang
J0 - Journal of Zhejiang University Science B
VL - 14
IS - 12
SP - 1100
EP - 1109
%@ 1673-1581
Y1 - 2013
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1300130

alkaloids are plant secondary metabolites that are widely distributed in Nicotiana species and contribute greatly to the quality of tobacco leaves. Some alkaloids, such as nornicotine and myosmine, have adverse effects on human health. To reduce the content of harmful alkaloids in tobacco leaves through conventional breeding, a genetic study of the alkaloid variation among different genotypes is required. In this study, alkaloid profiles in leaves of five Nicotiana tabacum cultivars and Nicotiana tomentosiformis were investigated. Six alkaloids were identified from all six genotypes via gas chromatograph-mass spectrometry (GC-MS). Significant differences in alkaloid content were observed both among different leaf positions and among cultivars. The contents of nornicotine and myosmine were positively and significantly correlated (R2=0.881), and were also separated from those of other alkaloids by clustering. Thus, the genotype plays a major role in alkaloid accumulation, indicating a high potential for manipulation of alkaloid content through traditional breeding.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1]Brogan, A.P., Dickerson, T.J., Boldt, G.E., Janda, K.D., 2005. Altered retinoid homeostasis catalyzed by a nicotine metabolite: implications in macular degeneration and normal development. PNAS, 102(30):10433-10438.

[2]Cai, J.B., Liu, B.Z., Lin, P., Su, Q.D., 2003. Fast analysis of nicotine related alkaloids in tobacco and cigarette smoke by megabore capillary gas chromatography. J. Chromatogr. A, 1017(1):187-193.

[3]Cai, J.L., Zhao, G., Yin, Y.Q., Zhou, H., Bian, Z.Y., Zhang, X.B., Xia, Q.B., Wang, B., Hu, B., Zhao, X.D., et al., 2011. Tobacco and Tobacco Products—Determination of Nicotine, Nornicotine, Anatabine, Myosmine and Anabasine—GC-MSD method. China Standard Press, Beijing, China, p.1-12 (in Chinese).

[4]Dickerson, T.J., Janda, K.D., 2002. A previously undescribed chemical link between smoking and metabolic disease. PNAS, 99(23):15084-15088.

[5]Djordjevic, M.V., Doran, K.A., 2009. Nicotine Content and Delivery across Tobacco Products. Nicotine Psychopharmacology, p.61-82.

[6]Djordjevic, M.V., Gay, S.L., Bush, L.P., Chaplin, J.F., 1989. Tobacco-specific nitrosamine accumulation and distribution in flue-cured tobacco alkaloid isolines. J. Agric. Food Chem., 37:752-756.

[7]Feng, H.J., Sun, J.L., Wang, J., Jia, Y.H., Zhang, X.Y., Pang, B.Y., Sun, J., Du, X.M., 2011. Genetic effects and heterosis of the fibre colour and quality of brown cotton (Gossypium hirsutum). Plant Breed., 130(4):450-456.

[8]Gavilano, L.B., Coleman, N.P., Burnley, L.E., Bowman, M.L., Kalengamaliro, N.E., Hayes, A., Bush, L., Siminszky, B., 2006. Genetic engineering of Nicotiana tabacum for reduced nornicotine content. J. Agric. Food Chem., 54(24):9071-9078.

[9]Gavilano, L.B., Coleman, N.P., Bowen, S.W., Siminszky, B., 2007. Functional analysis of nicotine demethylase genes reveals insights into the evolution of modern tobacco. J. Biol. Chem., 282(1):249-256.

[10]Häkkinen, S.T., Tilleman, S., Šwiątek, A., de Sutter, V., Rischer, H., Vanhoutte, I., van Onckelen, H., Hilson, P., Inzé, D., Oksman-Caldentey, K., et al., 2007. Functional characterization of genes involved in pyridine alkaloid biosynthesis in tobacco. Phytochemistry, 68(22-24):2773-2785.

[11]Hashimoto, T., Yamada, Y., 1994. Alkaloid biogenesis: molecular aspects. Annu. Rev. Plant Physiol. Plant Mol. Biol., 45(1):257-285.

[12]Hecht, S.S., 2003. Tobacco carcinogens, their biomarkers and tobacco-induced cancer. Nat. Rev. Cancer, 3(10):733-744.

[13]Hibi, N., Higashiguchi, S., Hashimoto, T., Yamada, Y., 1994. Gene expression in tobacco low-nicotine mutants. Plant Cell, 6(5):723-735.

[14]Leete, E., Chedekel, M.R., 1974. Metabolism of nicotine in Nicotiana glauca. Phytochemistry, 13(9):1853-1859.

[15]Lewis, R.S., 2006. Identification of germplasm of possible value for confronting an unfavorable inverse genetic correlation in tobacco. Crop Sci., 46(4):1764-1771.

[16]Lian, Y.Y., Wang, Y.B., Qiu, J., Zhang, Z.F., Cao, J.M., Ning, Y., Yue, S., Niu, P., 2008. Analysis on major alkaloids contents and constituent proportions of flue-cured tobacco from different tobacco production regions. Chin. Tobacco Sci., 29(4):6-9 (in Chinese).

[17]Lin, G.H., Zhou, J.H., Fan, Q.F., Yang, H.Q., Cao, R.X., 2002. Effects of topping technology on leaf yield, quality and alkaloid content of flue-cured tobacco. Chin. Tobacco Sci., 23(4):8-12 (in Chinese).

[18]Miller, R.G., 1974. The jackknife: a review. Biometrika, 61(1):1-15.

[19]Scherling, C., Ulrich, K., Ewald, D., Wechwerth, W., 2009. A metabolic signature of the beneficial interaction of the endophyte Paenibacillus sp. isolate and in vitro-grown poplar plants revealed by metabolomics. Mol. Plant Microbe Interact., 22(8):1032-1037.

[20]Shi, C.H., Xue, J.M., Yu, Y.G., Yang, X.E., Zhu, J., 1996. Analysis of genetic effects on nutrient quality traits in indica rice. Theor. Appl. Genet., 92(8):1099-1102.

[21]Shi, H.Z., Huang, Y.J., Liu, G.S., Zhao, M.Q., Bush, L.P., 2001. Alkaloid content and proportion in Chinese tobacco and cigarettes. Acta Tabacco Sin., 7(2):8-12 (in Chinese).

[22]Shi, H.Z., Li, J.P., Li, Z.P., Bush, L.P., Wang, C.J., Liu, G.S., 2007. Study on decreasing nicotine conversion in Chinese burley hybrid through genetic improvement. Sci. Agric. Sin., 40(1):153-160 (in Chinese).

[23]Shi, Q.M., Li, C.J., Zhang, F.S., 2006. Nicotine synthesis in Nicotiana tabacum L. induced by mechanical wounding is regulated by auxin. J. Exp. Bot., 57(11):2899-2907.

[24]Shoji, T., Kajikawa, M., Hashimoto, T., 2010. Clustered transcription factor genes regulate nicotine biosynthesis in tobacco. Plant Cell, 22(10):3390-3409.

[25]Siminszky, B., Gavilano, L., Bowen, S.W., Dewey, R.E., 2005. Conversion of nicotine to nornicotine in Nicotiana tabacum is mediated by CYP82E4, a cytochrome P450 monooxygenase. PNAS, 102(41):14919-14924.

[26]Wilp, J., Zwickenpflug, W., Richter, E., 2002. Nitrosation of dietary myosmine as risk factor of human cancer. Food Chem. Toxicol., 40(8):1223-1228.

[27]Yan, X.F., Chen, S.X., 2007. Regulation of plant glucosinolate metabolism. Planta, 226(6):1343-1352.

[28]Zador, E., Jones, D., 1986. The biosynthesis of a novel nicotine alkaloid in the trichomes of Nicotiana stocktonii. Plant Physiol., 82(2):479-484.

[29]Zhang, L., Wang, X., Guo, J., Xia, Q., Zhao, G., Zhou, H., Xie, F., 2013. Metabolic profiling of chinese tobacco leaf of different geographical origins by GC-MS. J. Agric. Food Chem., 61(11):2597-2605.

[30]Zhao, J.H., Zhang, J.S., Wang, Y., Wang, R.G., Wu, C., Fan, L.J., Ren, X.L., 2011. DNA methylation polymorphism in flue-cured tobacco and candidate markers for tobacco mosaic virus resistance. J. Zhejiang Univ.-Sci. B (Biomed. & Biotechnol.), 12(11):935-942.

[31]Zhu, J., 1996. Analytic methods for seed models with genotype× environment interactions. Acta Genet. Sin., 23(1):56-58 (in Chinese).

[32]Zhu, J., Weir, B.S., 1996. Diallel analysis for sex-linked and maternal effects. Theor. Appl. Genet., 92(1):1-9.

[33]Zwickenpflug, W., Meger, M., Richter, E., 1998. Occurrence of the tobacco alkaloid myosmine in nuts and nut products of Arachus hypogaea and Corylus avellana. J. Agric. Food Chem., 46(7):2703-2706.

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