Full Text:   <3132>

Summary:  <1947>

Suppl. Mater.: 

CLC number: Q522+.6

On-line Access: 2014-01-28

Received: 2013-07-01

Revision Accepted: 2013-08-25

Crosschecked: 2014-01-14

Cited: 5

Clicked: 6166

Citations:  Bibtex RefMan EndNote GB/T7714

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Journal of Zhejiang University SCIENCE B 2014 Vol.15 No.2 P.125-132


Analysis of promoters of microRNAs from a Glycine max degradome library* #

Author(s):  Yi-qiang Han1, Zheng Hu2, Dian-feng Zheng3, Ya-mei Gao1

Affiliation(s):  1. College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China; more

Corresponding email(s):   gaoym800@126.com

Key Words:  Glycine max , MicroRNA (miRNA), Promoter, Cis-acting element, Prediction

Yi-qiang Han, Zheng Hu, Dian-feng Zheng, Ya-mei Gao. Analysis of promoters of microRNAs from a Glycine max degradome library[J]. Journal of Zhejiang University Science B, 2014, 15(2): 125-132.

@article{title="Analysis of promoters of microRNAs from a Glycine max degradome library",
author="Yi-qiang Han, Zheng Hu, Dian-feng Zheng, Ya-mei Gao",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Analysis of promoters of microRNAs from a Glycine max degradome library
%A Yi-qiang Han
%A Zheng Hu
%A Dian-feng Zheng
%A Ya-mei Gao
%J Journal of Zhejiang University SCIENCE B
%V 15
%N 2
%P 125-132
%@ 1673-1581
%D 2014
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1300179

T1 - Analysis of promoters of microRNAs from a Glycine max degradome library
A1 - Yi-qiang Han
A1 - Zheng Hu
A1 - Dian-feng Zheng
A1 - Ya-mei Gao
J0 - Journal of Zhejiang University Science B
VL - 15
IS - 2
SP - 125
EP - 132
%@ 1673-1581
Y1 - 2014
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1300179

Objective: MicroRNAs (miRNAs) are genome-encoded, small non-coding RNAs that play important functions in development, biotic and abiotic stress responses, and other processes. Our aim was to explore the regulation of miRNA expression. Methods: We used bioinformatics methods to predict the core promoters of 440 miRNAs identified from a soybean (Glycine max) degradome library and to analyze cis-acting elements for 369 miRNAs. Results: The prediction results showed that 83.86% of the 440 miRNAs contained promoters in their upstream sequences, and 8.64% (38 loci) in their downstream sequences. The distributions of two core promoter elements, TATA-boxes and transcription start sites (TSSs), were similar. The cis-acting elements were examined to provide clues to the function and regulation of spatiotemporal expression of the miRNAs. Analyses of miRNA cis-elements and targets indicated a potential auxin response factor (ARF)- and gibberellin response factor (GARF)-mediated negative feedback loop for miRNA expression. Conclusions: The features of miRNAs from a Glycine max degradome library obtained here provide insights into the transcription regulation and functions of miRNAs in soybean.


研究目的:通过分析miRNA的核心启动子和顺式作用元件为进一步解析大豆(Glycine max)miRNAs表达调控及其功能研究提供重要信息。
研究方法:本研究利用TSSP 程序和PlantCARE数据库预测了来自大豆降解组文库的440个miRNA的核心启动子以及369个miRNAs的顺式作用元件,并依据顺式作用元件及靶基因构建miRNA调控网络。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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