CLC number: S571.1
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2015-01-08
Cited: 1
Clicked: 6145
Citations: Bibtex RefMan EndNote GB/T7714
http://orcid.org/0000-0001-6738-6663
Yue Zhang, Hai-peng Lv, Cheng-ying Ma, Li Guo, Jun-feng Tan, Qun-hua Peng, Zhi Lin. Cloning of a caffeoyl-coenzyme A O-methyltransferase from Camellia sinensis and analysis of its catalytic activity[J]. Journal of Zhejiang University Science B, 2015, 16(2): 103-112.
@article{title="Cloning of a caffeoyl-coenzyme A O-methyltransferase from Camellia sinensis and analysis of its catalytic activity",
author="Yue Zhang, Hai-peng Lv, Cheng-ying Ma, Li Guo, Jun-feng Tan, Qun-hua Peng, Zhi Lin",
journal="Journal of Zhejiang University Science B",
volume="16",
number="2",
pages="103-112",
year="2015",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1400193"
}
%0 Journal Article
%T Cloning of a caffeoyl-coenzyme A O-methyltransferase from Camellia sinensis and analysis of its catalytic activity
%A Yue Zhang
%A Hai-peng Lv
%A Cheng-ying Ma
%A Li Guo
%A Jun-feng Tan
%A Qun-hua Peng
%A Zhi Lin
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 2
%P 103-112
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400193
TY - JOUR
T1 - Cloning of a caffeoyl-coenzyme A O-methyltransferase from Camellia sinensis and analysis of its catalytic activity
A1 - Yue Zhang
A1 - Hai-peng Lv
A1 - Cheng-ying Ma
A1 - Li Guo
A1 - Jun-feng Tan
A1 - Qun-hua Peng
A1 - Zhi Lin
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 2
SP - 103
EP - 112
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400193
Abstract: Epigallocatechin-3-O-(3-O-methyl) gallate (EGCG3"Me) present in leaves of Camellia sinensis has many beneficial biological activities for human health. However, EGCG3"Me occurs naturally in tea leaves in extremely limited quantities. Finding an enzyme from C. sinensis to catalyze the synthesis of EGCG3"Me is an alternative method to make up for the scarcity of EGCG3"Me in natural situations. In the present study, a complementary DNA (cDNA) encoding region and genomic DNA of the caffeoyl-coenzyme A O-methyltransferase (CCoAOMT) gene were isolated from C. sinensis (designated CsCCoAOMT). Nucleotide sequence analysis of CsCCoAOMT revealed an open reading frame of 738 bp that encodes a polypeptide with a predicted molecular weight of 28 kDa, which correlated well with the results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The full-length DNA sequence (2678 bp) contained five exons and four introns. The deduced amino acid sequence of CsCCoAOMT shared 92% identity with CCoAOMTs from Codonopsis lanceolata and Betula luminifera. The catalytic activity of CsCCoAOMT was analyzed. Three monomethylated epigallocatechin-3-O-gallate (EGCG) compounds (EGCG4"Me, EGCG3"Me, and EGCG3'Me) were produced by CsCCoAOMT with Km in the micromolar range. Real-time polymerase chain reaction (RT-PCR) experiments indicated that the CsCCoAOMT transcript was present at low levels during the early stages of leaf maturity (the first leaf and bud on a shoot) but the relative expression was augmented at advanced stages of leaf maturity (the third or fourth leaf on a shoot), which accorded well with changes in EGCG3"Me content in fresh leaves. Hence, we concluded that CsCCoAOMT catalyzes the syntheses of methylated EGCGs.
[1]Bansal, S., Choudhary, S., Sharma, M., et al., 2013. Tea: a native source of antimicrobial agents. Food Res. Int., 53(2):568-584.
[2]Guillet-Claude, C., Birolleau-Touchard, C., Manicacci, D., et al., 2004. Genetic diversity associated with variation in silage corn digestibility for three O-methyltransferase genes involved in lignin biosynthesis. Theor. Appl. Genet., 110(1):126-135.
[3]Hoffmann, L., Maury, S., Bergdoll, M., et al., 2001. Identification of the enzymatic active site of tobacco caffeoyl-coenzyme A O-methyltransferase by site-directed mutagenesis. J. Biol. Chem., 276(39):36831-36838.
[4]Jankun, J., Selman, S.H., Swiercz, R., et al., 1997. Why drinking green tea could prevent cancer? Nature, 387(6633):561.
[5]Joshi, C., Chiang, V.L., 1998. Conserved sequence motifs in plant S-adenosyl-
[6]Keller, A.C., Weir, T.L., Broeckling, C.D., et al., 2013. Antibacterial activity and phytochemical profile of fermented Camellia sinensis (fuzhuan tea). Food Res. Int., 53(2):945-949.
[7]Khan, I.A., Awan, F.S., Ahmad, A., et al., 2004. A modified mini-prep method for economical and rapid extraction of genomic DNA in plants. Plant Mol. Biol. Rep., 22(1):89a-89e.
[8]Kim, B.G., Sung, S.H., Chong, Y., et al., 2010a. Plant flavonoid O-methyltransferases: substrate specificity and application. J. Plant Biol., 53(5):321-329.
[9]Kim, B.G., Kim, D.H., Sung, S.H., et al., 2010b. Two O-methyltransferases from Picea abies: characterization and molecular basis of different reactivity. Planta, 232(4):837-844.
[10]Kirita, M., Honma, D., Tanaka, Y., et al., 2010. Cloning of a novel O-methyltransferase from Camellia sinensis and synthesis of O-methylated EGCG and evaluation of their bioactivity. J. Agric. Food Chem., 58(12):7196-7201.
[11]Kuo, K.L., Weng, M.S., Chiang, C.T., et al., 2005. Comparative studies on the hypolipidemic and growth suppressive effects of oolong, black, pu-erh, and green tea leaves in rats. J. Agric. Food Chem., 53(2):480-489.
[12]Kurita, I., Maeda-Yamamoto, M., Tachibana, H., et al., 2010. Antihypertensive effect of Benifuuki tea containing O-methylated EGCG. J. Agric. Food Chem., 58(3):1903-1908.
[13]Lin, J.K., Lin-Shiau, S.Y., 2006. Mechanisms of hypolipidemic and anti-obesity effects of tea and tea polyphenols. Mol. Nutr. Food Res., 50(2):211-217.
[14]Livak, K.J., Schmittgen, T.D., 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods, 25(4):402-408.
[15]Lv, H.P., Lin, Z., Tan, J.F., et al., 2008. Study on EGCG3&quto;Me in tea. Food Ferment. Ind., 34(10):22-25 (in Chinese).
[16]Ma, C.L., Chen, L., Wang, X.C., et al., 2012. Differential expression analysis of different albescent stages of ‘Anji Baicha’ (Camellia sinensis (L.) O. Kuntze) using cDNA microarray. Sci. Hort., 148(4):246-254.
[17]Maeda-Yamamoto, M., Inagaki, N., Kitaura, J., et al., 2004. O-methylated catechins from tea leaves inhibit multiple protein kinases in mast cells. J. Immunol., 172(7):4486-4492.
[18]Maeda-Yamamoto, M., Ema, K., Shibuichi, I., 2007. In vitro and in vivo anti-allergic effects of ‘benifuuki’ green tea containing O-methylated catechin and ginger extract enhancement. Cytotechnology, 55(2-3):135-142.
[19]Mamati, G.E., Liang, Y.R., Lu, J.L., 2006. Expression of basic genes involved in tea polyphenol synthesis in relation to accumulation of catechins and total tea polyphenols. J. Sci. Food Agric., 86(3):459-464.
[20]Sano, M., Miyase, T., Tachibana, H., et al., 2000. Antiallergic properties of tea components. Frag. J., 4:46-52 (in Japanese).
[21]Singh, B.N., Shankar, S., Srivastava, R.K., 2011. Green tea catechin, epigallocatechin-3-gallate (EGCG): mechanisms, perspectives and clinical applications. Biochem. Pharmacol., 82(12):1807-1821.
[22]Zhao, H.Y., Sheng, Q.X., Lv, S.Y., et al., 2004. Characterization of three rice CCoAOMT genes. Chin. Sci. Bull., 49(15):1602-1606.
Open peer comments: Debate/Discuss/Question/Opinion
<1>