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Suppl. Mater.: 

CLC number: Q789

On-line Access: 2015-04-03

Received: 2014-07-19

Revision Accepted: 2014-11-06

Crosschecked: 2015-03-18

Cited: 3

Clicked: 4085

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Zheng Zhu

http://orcid.org/0000-0002-5379-9085

Ben-ke Kuai

http://orcid.org/0000-0003-0523-6381

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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.4 P.253-263

http://doi.org/10.1631/jzus.B1400203


Synthetic promoters consisting of defined cis-acting elements link multiple signaling pathways to probenazole-inducible system


Author(s):  Zheng Zhu, Jiong Gao, Jin-xiao Yang, Xiao-yan Wang, Guo-dong Ren, Yu-long Ding, Ben-ke Kuai

Affiliation(s):  The Southern Modern Forestry Collaborative Innovation Center, Nanjing Forestry University, Nanjing 210037, China; more

Corresponding email(s):   bkkuai@fudan.edu.cn

Key Words:  Probenazole, Systemic acquired resistance (SAR), Cis-acting element, Chemical-inducible system, Synthetic promoter


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Zheng Zhu, Jiong Gao, Jin-xiao Yang, Xiao-yan Wang, Guo-dong Ren, Yu-long Ding, Ben-ke Kuai. Synthetic promoters consisting of defined cis-acting elements link multiple signaling pathways to probenazole-inducible system[J]. Journal of Zhejiang University Science B, 2015, 16(4): 253-263.

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author="Zheng Zhu, Jiong Gao, Jin-xiao Yang, Xiao-yan Wang, Guo-dong Ren, Yu-long Ding, Ben-ke Kuai",
journal="Journal of Zhejiang University Science B",
volume="16",
number="4",
pages="253-263",
year="2015",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1400203"
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%A Guo-dong Ren
%A Yu-long Ding
%A Ben-ke Kuai
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Abstract: 
probenazole (3-allyloxy-1,2-benzisothiazole-1,1-dioxide, PBZ), the active component of Oryzemate, could induce systemic acquired resistance (SAR) in plants through the induction of salicylic acid (SA) biosynthesis. As a widely used chemical inducer, PBZ is a good prospect for establishing a new chemical-inducible system. We first designed artificially synthetic promoters with tandem copies of a single type of cis-element (SARE, JERE, GCC, GST1, HSRE, and W-box) that could mediate the expression of the β-glucuronidase (GUS) reporter gene in plants upon PBZ treatment. Then we combined different types of elements in order to improve inducibility in the PBZ-inducible system. On the other hand, we were surprised to find that the cis-elements, which are responsive to jasmonic acid (JA) and ethylene, also responded to PBZ, implying that SA, JA, and ethylene pathways also would play important roles in PBZ’s action. Further analysis demonstrated that PBZ also induced early events of innate immunity via a signaling pathway in which Ca2+ influx and mitogen-activated protein kinase (MAPK) activity were involved. We constructed synthesized artificial promoters to establish a PBZ chemical-inducible system, and preliminarily explored SA, JA, ethylene, calcium, and MAPK signaling pathways via PBZ-inducible system, which could provide an insight for in-depth study.

基于已知顺式元件的人工启动子响应性分析揭示烯丙异噻唑诱导系统可触发植物体内多条信号途径

中文概要:
目的:构建有效响应烯丙异噻唑(PBZ)诱导的人工合成启动子,了解植物体内受PBZ诱导系统触发的信号途径。
创新点:通过分析包含已知顺式元件的人工合成启动子对PBZ的响应性,为构建一种基于PBZ诱导系统的新型化学诱导启动子提供了可能性,并初步揭示了除水杨酸(SA)外,茉莉酸(JA)和乙烯等多条信号途径可能共同参与了PBZ诱导的植物免疫反应过程。
方法:利用已知的响应相关信号通路的顺式作用元件构建人工合成启动子,融合GUS报告基因后,稳定转化拟南芥。通过检测PBZ处理过程中GUS酶活性的变化,了解人工合成启动子对PBZ的响应性,分析PBZ诱导系统可能触发的信号途径。
结论:除了SA响应元件SARE可以有效响应PBZ诱导外,利用JA和乙烯响应元件JERE和GCC,超敏反应(HR)相关的顺式元件HSRE和GST1,以及植物抗病反应中重要顺式作用元件W-box构建的人工合成启动子也均可有效响应PBZ。另外,通过人工合成启动子响应性分析的手段,初步揭示了包括SA、JA、乙烯、钙离子和丝裂原活化蛋白激酶(MAPKs)在内的多条信号通路可能共同参与了PBZ诱导植物免疫反应的过程。

关键词:烯丙异噻唑;人工合成启动子;顺式作用元件;化学诱导系统

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