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CLC number: Q789

On-line Access: 2015-04-03

Received: 2014-07-19

Revision Accepted: 2014-11-06

Crosschecked: 2015-03-18

Cited: 3

Clicked: 4914

Citations:  Bibtex RefMan EndNote GB/T7714


Zheng Zhu


Ben-ke Kuai


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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.4 P.253-263


Synthetic promoters consisting of defined cis-acting elements link multiple signaling pathways to probenazole-inducible system

Author(s):  Zheng Zhu, Jiong Gao, Jin-xiao Yang, Xiao-yan Wang, Guo-dong Ren, Yu-long Ding, Ben-ke Kuai

Affiliation(s):  The Southern Modern Forestry Collaborative Innovation Center, Nanjing Forestry University, Nanjing 210037, China; more

Corresponding email(s):   bkkuai@fudan.edu.cn

Key Words:  Probenazole, Systemic acquired resistance (SAR), Cis-acting element, Chemical-inducible system, Synthetic promoter

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Zheng Zhu, Jiong Gao, Jin-xiao Yang, Xiao-yan Wang, Guo-dong Ren, Yu-long Ding, Ben-ke Kuai. Synthetic promoters consisting of defined cis-acting elements link multiple signaling pathways to probenazole-inducible system[J]. Journal of Zhejiang University Science B, 2015, 16(4): 253-263.

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author="Zheng Zhu, Jiong Gao, Jin-xiao Yang, Xiao-yan Wang, Guo-dong Ren, Yu-long Ding, Ben-ke Kuai",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

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%T Synthetic promoters consisting of defined cis-acting elements link multiple signaling pathways to probenazole-inducible system
%A Zheng Zhu
%A Jiong Gao
%A Jin-xiao Yang
%A Xiao-yan Wang
%A Guo-dong Ren
%A Yu-long Ding
%A Ben-ke Kuai
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 4
%P 253-263
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400203

T1 - Synthetic promoters consisting of defined cis-acting elements link multiple signaling pathways to probenazole-inducible system
A1 - Zheng Zhu
A1 - Jiong Gao
A1 - Jin-xiao Yang
A1 - Xiao-yan Wang
A1 - Guo-dong Ren
A1 - Yu-long Ding
A1 - Ben-ke Kuai
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 4
SP - 253
EP - 263
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400203

probenazole (3-allyloxy-1,2-benzisothiazole-1,1-dioxide, PBZ), the active component of Oryzemate, could induce systemic acquired resistance (SAR) in plants through the induction of salicylic acid (SA) biosynthesis. As a widely used chemical inducer, PBZ is a good prospect for establishing a new chemical-inducible system. We first designed artificially synthetic promoters with tandem copies of a single type of cis-element (SARE, JERE, GCC, GST1, HSRE, and W-box) that could mediate the expression of the β-glucuronidase (GUS) reporter gene in plants upon PBZ treatment. Then we combined different types of elements in order to improve inducibility in the PBZ-inducible system. On the other hand, we were surprised to find that the cis-elements, which are responsive to jasmonic acid (JA) and ethylene, also responded to PBZ, implying that SA, JA, and ethylene pathways also would play important roles in PBZ’s action. Further analysis demonstrated that PBZ also induced early events of innate immunity via a signaling pathway in which Ca2+ influx and mitogen-activated protein kinase (MAPK) activity were involved. We constructed synthesized artificial promoters to establish a PBZ chemical-inducible system, and preliminarily explored SA, JA, ethylene, calcium, and MAPK signaling pathways via PBZ-inducible system, which could provide an insight for in-depth study.




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