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Abstract: probenazole (3-allyloxy-1,2-benzisothiazole-1,1-dioxide, PBZ), the active component of Oryzemate, could induce systemic acquired resistance (SAR) in plants through the induction of salicylic acid (SA) biosynthesis. As a widely used chemical inducer, PBZ is a good prospect for establishing a new chemical-inducible system. We first designed artificially synthetic promoters with tandem copies of a single type of cis-element (SARE, JERE, GCC, GST1, HSRE, and W-box) that could mediate the expression of the β-glucuronidase (GUS) reporter gene in plants upon PBZ treatment. Then we combined different types of elements in order to improve inducibility in the PBZ-inducible system. On the other hand, we were surprised to find that the cis-elements, which are responsive to jasmonic acid (JA) and ethylene, also responded to PBZ, implying that SA, JA, and ethylene pathways also would play important roles in PBZ’s action. Further analysis demonstrated that PBZ also induced early events of innate immunity via a signaling pathway in which Ca²⁺ influx and mitogen-activated protein kinase (MAPK) activity were involved. We constructed synthesized artificial promoters to establish a PBZ chemical-inducible system, and preliminarily explored SA, JA, ethylene, calcium, and MAPK signaling pathways via PBZ-inducible system, which could provide an insight for in-depth study.

基于已知顺式元件的人工启动子响应性分析揭示烯丙异噻唑诱导系统可触发植物体内多条信号途径

[1]Alvarez, M.E., Pennell, R.I., Meijer, P.J., et al., 1998. Reactive oxygen intermediates mediate a systemic signal network in the establishment of plant immunity. Cell, 92(6):773-784.

[2]Asai, T., Tena, G., Plotnikova, J., et al., 2002. MAP kinase signalling cascade in Arabidopsis innate immunity. Nature, 415(6875):977-983.

[3]Camargo, A., Llamas, A., Schnell, R.A., et al., 2007. Nitrate signaling by the regulatory gene NIT2 in Chlamydomonas. Plant Cell, 19(11):3491-3503.

[4]Clough, S.J., Bent, A.F., 1998. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J., 16(6):735-743.

[5]Gozzo, F., 2003. Systemic acquired resistance in crop protection: from nature to a chemical approach. J. Agric. Food Chem., 51(16):4487-4503.

[6]Gurr, S.J., Rushton, P.J., 2005. Engineering plants with increased disease resistance: how are we going to express it? Trends Biotechnol., 23(6):283-290.

[7]Hammond-Kosack, K.E., Parker, J.E., 2003. Deciphering plant-pathogen communication: fresh perspectives for molecular resistance breeding. Curr. Opin. Biotechnol., 14(2):177-193.

[8]Heath, M.C., 2000. Hypersensitive response-related death. Plant Mol. Biol., 44(3):321-334.

[9]Iwai, T., Seo, S., Mitsuhara, I., et al., 2007. Probenazole-induced accumulation of salicylic acid confers resistance to Magnaporthe grisea in adult rice plants. Plant Cell Physiol., 48(7):915-924.

[10]Iwata, M., Umemura, K., Midoh, N., 2004. Probenazole (Oryzemate^®): a plant defense activator. In: Kawasaki, S. (Ed.), Rice Blast: Interaction with Rice and Control: Proceedings of the 3rd International Rice Blast Conference. Springer Netherlands, p.163-171.

[11]Jefferson, R.A., Kavanagh, T.A., Bevan, M.W., 1987. GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher-plants. EMBO J., 6(13):3901-3907.

[12]Kirsch, C., Takamiya-Wik, M., Schmelzer, E., et al., 2000. A novel regulatory element involved in rapid activation of parsley ELI7 gene family members by fungal elicitor or pathogen infection. Mol. Plant Pathol., 1(4):243-251.

[13]Levine, A., Tenhaken, R., Dixon, R., et al., 1994. H₂O₂ from the oxidative burst orchestrates the plant hypersensitive disease resistance response. Cell, 79(4):583-593.

[14]Ma, W., Berkowitz, G.A., 2007. The grateful dead: calcium and cell death in plant innate immunity. Cell. Microbiol., 9(11):2571-2585.

[15]Mehrotra, R., Gupta, G., Sethi, R., et al., 2011. Designer promoter: an artwork of cis engineering. Plant Mol. Biol., 75(6):527-536.

[16]Menke, F.L.H., Champion, A., Kijne, J.W., et al., 1999. A novel jasmonate- and elicitor-responsive element in the periwinkle secondary metabolite biosynthetic gene Str interacts with a jasmonate- and elicitor-inducible AP2-domain transcription factor, ORCA2. EMBO J., 18(16):4455-4463.

[17]Midoh, N., Iwata, M., 1996. Cloning and characterization of a probenazole-inducible gene for an intracellular pathogenesis-related protein in rice. Plant Cell Physiol., 37(1):9-18.

[18]Midoh, N., Iwata, M., 1997. Expression of defense-related genes by probenazole or 1,2-benzisothiazole-3(2H)-one 1,1-dioxide. J. Pesticide Sci., 22(1):45-47.

[19]Nakashita, H., Yoshioka, K., Yasuda, M., et al., 2002. Probenazole induces systemic acquired resistance in tobacco through salicylic acid accumulation. Physiol. Mol. Plant Pathol., 61(4):197-203.

[20]Nemhauser, J.L., Hong, F.X., Chory, J., 2006. Different plant hormones regulate similar processes through largely nonoverlapping transcriptional responses. Cell, 126(3):467-475.

[21]Nikolaev, O.N., Aver'yanov, A.A., 1991. Involvement of superoxide radical in the mechanism of fungicidic action of phthalide and probenazol. Soviet Plant Physiol., 38(3):375-381.

[22]Ohme-Takagi, M., Suzuki, K., Shinshi, H., 2000. Regulation of ethylene-induced transcription of defense genes. Plant Cell Physiol., 41(11):1187-1192.

[23]Penninckx, I.A.M.A., Eggermont, K., Terras, F.R.G., et al., 1996. Pathogen-induced systemic activation of a plant defensin gene in Arabidopsis follows a salicylic acid-independent pathway. Plant Cell, 8(12):2309-2323.

[24]Pontier, D., Balague, C., Bezombes-Marion, I., et al., 2001. Identification of a novel pathogen-responsive element in the promoter of the tobacco gene HSR203J, a molecular marker of the hypersensitive response. Plant J., 26(5):495-507.

[25]Roslan, H.A., Salter, M.G., Wood, C.D., et al., 2001. Characterization of the ethanol-inducible alc gene-expression system in Arabidopsis thaliana. Plant J., 28(2):225-235.

[26]Rushton, P.J., Somssich, I.E., 1998. Transcriptional control of plant genes responsive to pathogens. Curr. Opin. Plant Biol., 1(4):311-315.

[27]Rushton, P.J., Reinstadler, A., Lipka, V., et al., 2002. Synthetic plant promoters containing defined regulatory elements provide novel insights into pathogen- and wound-induced signaling. Plant Cell, 14(4):749-762.

[28]Rushton, P.J., Somssich, I.E., Ringler, P., et al., 2010. WRKY transcription factors. Trends Plant Sci., 15(5):247-258.

[29]Sakamoto, K., Tada, Y., Yokozeki, Y., et al., 1999. Chemical induction of disease resistance in rice is correlated with the expression of a gene encoding a nucleotide binding site and leucine-rich repeats. Plant Mol. Biol., 40(5):847-855.

[30]Sasaki, K., Iwai, T., Hiraga, S., et al., 2004. Ten rice peroxidases redundantly respond to multiple stresses including infection with rice blast fungus. Plant Cell Physiol., 45(10):1442-1452.

[31]Sekizawa, Y., Watanabe, T., 1981. On the mode of action of probenazole against rice blast. J. Pesticide Sci., 6(2):247-255.

[32]Serrano, M., Guzman, P., 2004. Isolation and gene expression analysis of Arabidopsis thaliana mutants with constitutive expression of ATL2, an early elicitor-response RING-H2 zinc-finger gene. Genetics, 167(2):919-929.

[33]Shah, J., Klessig, D.F., 1996. Identification of a salicylic acid-responsive element in the promoter of the tobacco pathogenesis-related β-1,3-glucanase gene, PR-2d. Plant J., 10(6):1089-1101.

[34]Strittmatter, G., Gheysen, G., Gianinazzipearson, V., et al., 1996. Infections with various types of organisms stimulate transcription from a short promoter fragment of the potato gst1 gene. Mol. Plant-Microbe Interact., 9(1):68-73.

[35]Ton, J., Mauch-Mani, B., 2004. β-amino-butyric acid-induced resistance against necrotrophic pathogens is based on ABA-dependent priming for callose. Plant J., 38(1):119-130.

[36]Torres, M.A., Jones, J.D., Dangl, J.L., 2006. Reactive oxygen species signaling in response to pathogens. Plant Physiol., 141(2):373-378.

[37]Venter, M., 2007. Synthetic promoters: genetic control through cis engineering. Trends Plant Sci., 12(3):118-124.

[38]Watanabe, T., 1977. Effects of probenazole (Oryzemate^®) on each stage of rice blast fungus (Pyricularia oryzae Cavara) in its life cycle. J. Pesticide Sci., 2(4):395-404.

[39]Yasuda, M., Ishikawa, A., Jikumaru, Y., et al., 2008. Antagonistic interaction between systemic acquired resistance and the abscisic acid-mediated abiotic stress response in Arabidopsis. Plant Cell, 20(6):1678-1692.

[40]Yoshioka, K., Nakashita, H., Klessig, D.F., et al., 2001. Probenazole induces systemic acquired resistance in Arabidopsis with a novel type of action. Plant J., 25(2):149-157.

[41]Yu, J., Wang, X.Y., Wei, Q.A., et al., 2010a. Identification of regulatory cis-elements upstream of AtNPR1 that are responsive to probenazole treatment in transgenic tobacco plants. J. Plant Biol., 53(4):282-290.

[42]Yu, J., Gao, J.O., Wang, X.Y., et al., 2010b. The pathway and regulation of salicylic acid biosynthesis in probenazole-treated Arabidopsis. J. Plant Biol., 53(6):417-424.

[43]Zhang, A.Y., Jiang, M.Y., Zhang, J.H., et al., 2006. Mitogen-activated protein kinase is involved in abscisic acid-induced antioxidant defense and acts downstream of reactive oxygen species production in leaves of maize plants. Plant Physiol., 141(2):475-487.