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CLC number: S435.121.4+2

On-line Access: 2015-08-04

Received: 2014-12-23

Revision Accepted: 2015-04-22

Crosschecked: 2015-07-09

Cited: 2

Clicked: 2957

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Gang-ming Zhan

http://orcid.org/0000-0001-9282-9606

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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.8 P.727-732

http://doi.org/10.1631/jzus.B1400364


Screening for simple sequence repeat markers in Puccinia striiformis tritici based on genomic sequence


Author(s):  Gang-ming Zhan, Fu-ping Wang, Huai-yong Luo, Shu-chang Jiang, Wen-ming Zheng, Li-li Huang, Zhen-sheng Kang

Affiliation(s):  1State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling 712100, China; more

Corresponding email(s):   kangzs@nwsuaf.edu.cn

Key Words:  Microsatellites, Simple sequence repeat, Stripe rust


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Gang-ming Zhan, Fu-ping Wang, Huai-yong Luo, Shu-chang Jiang, Wen-ming Zheng, Li-li Huang, Zhen-sheng Kang. Screening for simple sequence repeat markers in Puccinia striiformis tritici based on genomic sequence[J]. Journal of Zhejiang University Science B, 2015, 16(8): 727-732.

@article{title="Screening for simple sequence repeat markers in Puccinia striiformis tritici based on genomic sequence",
author="Gang-ming Zhan, Fu-ping Wang, Huai-yong Luo, Shu-chang Jiang, Wen-ming Zheng, Li-li Huang, Zhen-sheng Kang",
journal="Journal of Zhejiang University Science B",
volume="16",
number="8",
pages="727-732",
year="2015",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1400364"
}

%0 Journal Article
%T Screening for simple sequence repeat markers in Puccinia striiformis tritici based on genomic sequence
%A Gang-ming Zhan
%A Fu-ping Wang
%A Huai-yong Luo
%A Shu-chang Jiang
%A Wen-ming Zheng
%A Li-li Huang
%A Zhen-sheng Kang
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 8
%P 727-732
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400364

TY - JOUR
T1 - Screening for simple sequence repeat markers in Puccinia striiformis tritici based on genomic sequence
A1 - Gang-ming Zhan
A1 - Fu-ping Wang
A1 - Huai-yong Luo
A1 - Shu-chang Jiang
A1 - Wen-ming Zheng
A1 - Li-li Huang
A1 - Zhen-sheng Kang
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 8
SP - 727
EP - 732
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400364


Abstract: 
Puccinia striiformis f. sp. tritici (Pst) is the obligate biotrophic fungus responsible for stripe rust wheat. In this study, we developed and characterized 20 polymorphic microsatellite markers from the genomic sequence of an isolate of Chinese Pst race CY32. Polymorphism at each simple sequence repeat (SSR) locus was determined using 32 Pst isolates from 7 countries. The number of alleles varied from 2 to 7 across isolates, and the observed and expected heterozygosities ranged from 0.33 to 0.97 (mean 0.62) and 0.23 to 0.73 (mean 0.51), respectively. As expected the genomic SSR markers were more polymorphic than the expressed sequence tag (EST)-SSR markers developed previously. These markers will be more useful for population genetics and molecular genetics studies in Pst.

基于小麦条锈菌基因组序列筛选简单重复序列 标记

目的:筛选得到多态性好、扩增稳定的条锈菌微卫星标记,以期更好应用于小麦条锈病菌的群体遗传学研究。
创新点:基于条锈菌基因组设计了100对候选简单重复序列(SSR)引物,最终筛选得到20对多态性好并且扩增稳定的微卫星标记。
方法:基于条锈菌100个组装序列用Primer 5软件设计了100对SSR引物用于检测来自7个国家的32个菌系,最终筛选得到20对较好的微卫星标记(表2)。
结论:基于基因组序列筛选得到的20对微卫星标记将会在小麦条锈菌群体遗传研究中得到广泛应用。

关键词:微卫星标记;简单重复序列;小麦条锈菌

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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